In vitro effects of adrenomedullin and calcitonin gene related peptide on the release of serotonin and amino acids from rat dorsal spinal cord

2007 ◽  
Vol 420 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Mohammad Ali Takhshid ◽  
Ali Akbar Owji ◽  
Mohammad Reza Panjehshahin
Keyword(s):  
Amino Acids ◽  
Spinal Cord ◽  
Calcitonin Gene Related Peptide ◽  
Dorsal Spinal Cord ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
In Vitro Effects ◽  
Dorsal Spinal

Plasticity of calcitonin gene related peptide neurotransmission in the spinal cord during peripheral inflammation

1995 ◽  
Vol 73 (7) ◽  
pp. 1007-1014 ◽  
Author(s):  
V. S. Seybold ◽  
M. T. Galeazza ◽  
M. G. Garry ◽  
K. M. Hargreaves
Keyword(s):  
Spinal Cord ◽  
Calcitonin Gene Related Peptide ◽  
Peripheral Inflammation ◽  
Afferent Neurons ◽  
Dorsal Spinal Cord ◽  
Primary Afferent Neurons ◽  
Primary Afferent ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Dorsal Spinal

Injection of complete Freund's adjuvant (CFA; 75 μL) into the plantar surface of the hind paw of the rat results in a mild inflammation that lasts for several days and is accompanied by hyperalgesia. Multiple components of calcitonin gene related peptide (CGRP) neurotransmission in the spinal cord are altered during the course of this peripheral inflammation. The content of immunoreactive (i) CGRP in the dorsal horn of the spinal cord, where primary afferent neurons terminate, is significantly decreased within 2 days after injection of CFA but increases to a level greater than that of the control at 8 days. The early decrease in iCGRP in the spinal cord suggests that the release of CGRP from primary afferent neurons is increased during the period of maximal hyperalgesia that accompanies peripheral inflammation. Changes in the mRNA for CGRP suggest that the increase in spinal content of iCGRP is due to an increase in synthesis of the peptide as the level of mRNA for CGRP is increased from 2 to 8 days after injection of CFA. Despite the decrease in the content of iCGRP in the spinal cord, there is no apparent decrease in the amount of iCGRP that can be released from the dorsal spinal cord by capsaicin; in fact, capsaicin-evoked release is increased at 4 days. Measurements of the binding of 125I-labelled CGRP in the dorsal spinal cord indicate that high affinity binding sites for CGRP are downregulated at 4 days after injection of CFA. In total, these data support the hypothesis that the activity of CGRP-containing primary afferent neurons is increased during peripheral inflammation. CGRP released from primary afferent neurons in the spinal cord may contribute to cellular changes that accompany peripheral inflammation.Key words: calcitonin gene related peptide, dorsal root ganglion, hyperalgesia, inflammation, spinal cord.

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