Immunohistochemical analysis of nuclear lamins in the glial cells of adult rat brain

The central nervous system has a limited capacity for self-repair after damage. However, the neonatal brain has agreater capacity for recovery than the adult brain. These differences in the regenerative capability depend on local environmental factors and the maturational stage of growing axons. Among molecules which have both growth-promoting and growth-inhibiting activities is the heterogeneous class of chondroitin sulfate proteoglycans (CSPGs). In this paper, we investigated the chondroitin-4 and chondroitin-6 sulfate proteoglycan expression profile after left sensorimotor cortex ablation of the neonatal and adult rat brain. Immunohistochemical analysis revealed that compared to the normal uninjured cortex, lesion provoked up regulation of CSPGs showing a different pattern of expression in the neonatal vs. the adult brain. Punctuate and membrane-bound labeling was predominate after neonatal lesion, where as heavy deposition of staining in the extracellular matrix was observed after adult lesion. Heavy deposition of CSPG immunoreactivity around the lesionsite in adult rats, in contrast to a less CSPG-rich environment in neonatal rats, indicated that enhancement of the recovery process after neonatal injury is due to amore permissive environment.

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Regional distribution of 5α-reductase type 2 in the adult rat brain: An immunohistochemical analysis

Psychoneuroendocrinology ◽

10.1016/j.psyneuen.2012.06.008 ◽

2013 ◽

Vol 38 (2) ◽

pp. 281-293 ◽

Cited By ~ 42

Author(s):

M. Paola Castelli ◽

Alberto Casti ◽

Angelo Casu ◽

Roberto Frau ◽

Marco Bortolato ◽

...

Keyword(s):

Rat Brain ◽

Regional Distribution ◽

Immunohistochemical Analysis ◽

Adult Rat ◽

Adult Rat Brain

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Glial cells from normal adult rat brain established in continuous culture

Brain Research ◽

10.1016/0006-8993(78)91041-7 ◽

1978 ◽

Vol 155 (2) ◽

pp. 418-421 ◽

Cited By ~ 33

Author(s):

Vijendra K. Singh ◽

Diane Van Alstyne

Keyword(s):

Continuous Culture ◽

Rat Brain ◽

Glial Cells ◽

Normal Adult ◽

Adult Rat ◽

Adult Rat Brain

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Histochemical demonstration of purine nucleoside phosphorylase (PNPase) in microglial and astroglial cells of adult rat brain.

Journal of Histochemistry & Cytochemistry ◽

10.1177/38.11.2120328 ◽

1990 ◽

Vol 38 (11) ◽

pp. 1535-1539 ◽

Cited By ~ 16

Author(s):

B Castellano ◽

B González ◽

B R Finsen ◽

J Zimmer

Keyword(s):

Rat Brain ◽

Glial Cells ◽

Purine Nucleoside Phosphorylase ◽

Purine Nucleoside ◽

Microglial Cells ◽

Histochemical Localization ◽

Astroglial Cells ◽

Nucleoside Phosphorylase ◽

Adult Rat ◽

Adult Rat Brain

The histochemical localization of enzymes associated with purine nucleoside metabolism indicates that glial cells might participate in the regulation of these compounds in the central nervous system. In the present study we examined the histochemical localization of purine nucleoside phosphorylase (PNPase) in sections from adult rat brain. Some sections were also sequentially stained immunocytochemically for astroglial or microglial cells utilizing glial fibrillary acidic protein (GFAP) or OX-42 antibodies, respectively. Our observations showed that PNPase was restricted to glial cells, whereas neurons always remained negative. Brain sections stained for both PNPase and GFAP showed that the GFAP-positive astroglial cells were always PNPase positive. Other PNPase-positive but GFAP-negative cells were also observed. These cells resembled microglial cells, and brain sections reacted for both PNPase and OX-42 confirmed this by showing that the major part of OX-42-positive microglial cells were PNPase positive. In these sections, the PNPase-positive but OX-42-negative cells present resembled astroglial cells. From our double staining experiments, we conclude that PNPase is present in both astroglial and microglial cells in normal adult brain.

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Heat shock induces c-fos protein-like immunoreactivity in glial cells in adult rat brain

Experimental Neurology ◽

10.1016/0014-4886(89)90152-0 ◽

1989 ◽

Vol 106 (1) ◽

pp. 105-109 ◽

Cited By ~ 29

Author(s):

M. Dragunow ◽

R.W. Currie ◽

H.A. Robertson ◽

R.L.M. Faull

Keyword(s):

Heat Shock ◽

Rat Brain ◽

Glial Cells ◽

Adult Rat ◽

Fos Protein ◽

Adult Rat Brain

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Distribution of Aquaporin 9 in the adult rat brain: Preferential expression in catecholaminergic neurons and in glial cells

Neuroscience ◽

10.1016/j.neuroscience.2004.05.042 ◽

2004 ◽

Vol 128 (1) ◽

pp. 27-38 ◽

Cited By ~ 111

Author(s):

J. Badaut ◽

J.-M. Petit ◽

J.-F. Brunet ◽

P.J. Magistretti ◽

C. Charriaut-Marlangue ◽

...

Keyword(s):

Rat Brain ◽

Glial Cells ◽

Preferential Expression ◽

Catecholaminergic Neurons ◽

Adult Rat ◽

Aquaporin 9 ◽

Adult Rat Brain

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Demonstration of poly-N-acetyl lactosamine residues in ameboid and ramified microglial cells in rat brain by tomato lectin binding.

Journal of Histochemistry & Cytochemistry ◽

10.1177/42.8.8027523 ◽

1994 ◽

Vol 42 (8) ◽

pp. 1033-1041 ◽

Cited By ~ 152

Author(s):

L Acarin ◽

J M Vela ◽

B González ◽

B Castellano

Keyword(s):

Rat Brain ◽

Glial Cells ◽

Lectin Histochemistry ◽

Adult Brain ◽

Microglial Cells ◽

Electron Microscopic Level ◽

Sugar Residue ◽

Adult Rat ◽

Tomato Lectin ◽

Adult Rat Brain

This study was designed to demonstrate the localization of poly-N-acetyl lactosamine residues in postnatal and adult rat brain, visualized by their specific binding to a lectin obtained from Lycopersicon esculentum (tomato). Lectin histochemistry was carried out on cryostat, paraffin, and vibratome sections and was examined by light microscopy. Selected vibratome sections were processed for electron microscopy. Our results showed that tomato lectin histochemistry was found in relation to blood vessels and glial cells in both postnatal and adult rat brain. Since tomato lectin-positive glial cells did not show GFAP immunoreactivity and displayed the same morphological features and overall distribution as nucleoside diphosphatase (NDPase)-positive cells, they were consequently identified as microglial cells. At the electron microscopic level, both ameboid and ramified microglial cells displayed intracytoplasmic and plasma membrane lectin reactivity. In postnatal brain, ameboid microglial cells always showed stronger binding of tomato lectin compared with ramified microglial cells in the adult brain. The putative significance of this decrease in poly-N-acetyl lactosamine from ameboid to ramified microglial cells and the possible role(s) of this sugar residue are discussed.

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