Neural differentiation of mouse embryonic stem cells as a tool to assess developmental neurotoxicity in vitro

2012 ◽  
Vol 33 (5) ◽  
pp. 1135-1146 ◽  
Author(s):  
Anke Visan ◽  
Katrin Hayess ◽  
Dana Sittner ◽  
Elena E. Pohl ◽  
Christian Riebeling ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Developmental Neurotoxicity

scholarly journals Assessment of the developmental neurotoxicity of silver nanoparticles and silver ions with mouse embryonic stem cells in vitro

2018 ◽  
Vol 3 (3) ◽  
pp. 133-145 ◽  
Author(s):  
Nuoya Yin ◽  
Bowen Hu ◽  
Renjun Yang ◽  
Shaojun Liang ◽  
Shengxian Liang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Silver Nanoparticles ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Silver Ions ◽  
Mouse Embryonic Stem Cells ◽  
Developmental Neurotoxicity

SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc- SMPDL3B

2021 ◽  
Author(s):  
Wei Fan ◽  
Shuang Tang ◽  
Xiaojuan Fan ◽  
Yi Fang ◽  
Xiaojiang Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Sphingolipid Metabolism

AbstractSphingolipids are important structural components of cell membranes and prominent signaling molecules controlling cell growth, differentiation, and apoptosis. Sphingolipids are particularly abundant in the brain, and defects in sphingolipid degradation are associated with several human neurodegenerative diseases. However, molecular mechanisms governing sphingolipid metabolism remain unclear. Here we report that sphingolipid degradation is under transcriptional control of SIRT1, a highly conserved mammalian NAD+-dependent protein deacetylase, in mouse embryonic stem cells (mESCs). Deletion of SIRT1 results in accumulation of sphingomyelin in mESCs, primarily due to reduction of SMPDL3B, a GPI-anchored plasma membrane bound sphingomyelin phosphodiesterase. Mechanistically, SIRT1 regulates transcription of Smpdl3b through c-Myc. Functionally, SIRT1 deficiency-induced accumulation of sphingomyelin increases membrane fluidity and impairs neural differentiation in vitro and in vivo. Our findings discover a key regulatory mechanism for sphingolipid homeostasis and neural differentiation, further imply that pharmacological manipulation of SIRT1-mediated sphingomyelin degradation might be beneficial for treatment of human neurological diseases.

scholarly journals SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc- SMPDL3B

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Wei Fan ◽  
Shuang Tang ◽  
Xiaojuan Fan ◽  
Yi Fang ◽  
Xiaojiang Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Sphingolipid Metabolism

Sphingolipids are important structural components of cell membranes and prominent signaling molecules controlling cell growth, differentiation, and apoptosis. Sphingolipids are particularly abundant in the brain, and defects in sphingolipid degradation are associated with several human neurodegenerative diseases. However, molecular mechanisms governing sphingolipid metabolism remain unclear. Here we report that sphingolipid degradation is under transcriptional control of SIRT1, a highly conserved mammalian NAD+-dependent protein deacetylase, in mouse embryonic stem cells (mESCs). Deletion of SIRT1 results in accumulation of sphingomyelin in mESCs, primarily due to reduction of SMPDL3B, a GPI-anchored plasma membrane bound sphingomyelin phosphodiesterase. Mechanistically, SIRT1 regulates transcription of Smpdl3b through c-Myc. Functionally, SIRT1 deficiency-induced accumulation of sphingomyelin increases membrane fluidity and impairs neural differentiation in vitro and in vivo. Our findings discover a key regulatory mechanism for sphingolipid homeostasis and neural differentiation, further imply that pharmacological manipulation of SIRT1-mediated sphingomyelin degradation might be beneficial for treatment of human neurological diseases.

Assessment of malathion developmental neurotoxicity in vitro with mouse embryonic stem cells

2016 ◽  
Vol 258 ◽  
pp. S240-S241
Author(s):  
M. Rezvanfar ◽  
A. Atashi ◽  
M. Salehnia ◽  
M. Baeeri ◽  
M. Abdollahi
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Developmental Neurotoxicity

The long-term survival of in vitro engineered nervous tissue derived from the specific neural differentiation of mouse embryonic stem cells

Biomaterials ◽  
2010 ◽  
Vol 31 (27) ◽  
pp. 7032-7042 ◽  
Author(s):  
Michel L. Dubois-Dauphin ◽  
Nicolas Toni ◽  
Stéphanie D. Julien ◽  
Igor Charvet ◽  
Lars E. Sundstrom ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Nervous Tissue ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Term Survival ◽  
Long Term Survival

scholarly journals Creation of Engineered Cardiac Tissue In Vitro From Mouse Embryonic Stem Cells

Circulation ◽  
2006 ◽  
Vol 113 (18) ◽  
pp. 2229-2237 ◽  
Author(s):  
Xi-Min Guo ◽  
Yun-Shan Zhao ◽  
Hai-Xia Chang ◽  
Chang-Yong Wang ◽  
Ling-Ling E ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cardiac Tissue ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

Differentiation of E14 Mouse Embryonic Stem Cells into Thyrocytes In Vitro

Thyroid ◽  
2010 ◽  
Vol 20 (1) ◽  
pp. 77-84 ◽  
Author(s):  
Ningyi Jiang ◽  
Yingying Hu ◽  
Xiongying Liu ◽  
Yanfeng Wu ◽  
Hong Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells

scholarly journals Development of Antiviral Innate Immunity During In Vitro Differentiation of Mouse Embryonic Stem Cells

2016 ◽  
Vol 25 (8) ◽  
pp. 648-659 ◽  
Author(s):  
William D'Angelo ◽  
Dhiraj Acharya ◽  
Ruoxing Wang ◽  
Jundi Wang ◽  
Chandan Gurung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Innate Immunity ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
In Vitro Differentiation ◽  
Antiviral Innate Immunity

Influence of random and oriented electrospun fibrous poly(lactic-co -glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells

2017 ◽  
Vol 105 (5) ◽  
pp. 1333-1345 ◽  
Author(s):  
Laura E. Sperling ◽  
Karina P. Reis ◽  
Laura G. Pozzobon ◽  
Carolina S. Girardi ◽  
Patricia Pranke
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Glycolic Acid ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells
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