Hydrogen sulphide in the hypothalamus causes an ATP-sensitive K+ channel-dependent decrease in blood pressure in freely moving rats

When injected into specific rat brain regions, the neurotransmitter candidate L-proline produces various cardiovascular changes through ionotropic excitatory amino acid receptors. The present study used an immunohistochemical double-labeling approach to determine whether intracisternally injected L-proline in freely moving rats, which increases blood pressure, activates hypothalamic vasopressin-expressing neurons and ventral medullary tyrosine-hydroxylase- (TH-) containing neurons. Following injection of L-proline, the number of activated hypothalamic neurons that coexpressed vasopressin and c-Fos was much greater in the supraoptic nucleus (SON) than in the paraventricular nucleus (PVN) of rats with increased blood pressure. The number of activated TH-containing neurons was significantly greater following L-proline treatment than following control injections of artificial cerebrospinal fluid (ACSF). These results clearly demonstrate that intracisternally injected L-proline activates hypothalamic supraoptic, but not paraventricular, vasopressin-expressing neurons and medullary TH-containing (A1/C1) neurons in freely moving rats.

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Use of Telemetry Blood Pressure Transmitters to Measure Intracranial Pressure (ICP) in Freely Moving Rats

Current Neurovascular Research ◽

10.2174/156720209787466046 ◽

2009 ◽

Vol 6 (1) ◽

pp. 62-69 ◽

Cited By ~ 28

Author(s):

Gergely Silasi ◽

Crystal MacLellan ◽

Frederick Colbourne

Keyword(s):

Blood Pressure ◽

Intracranial Pressure ◽

Freely Moving Rats ◽

Freely Moving

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Blood Pressure Measurement in Freely Moving Rats by the Tail Cuff Method

Clinical and Experimental Hypertension ◽

10.3109/10641963.2012.685534 ◽

2012 ◽

Vol 35 (1) ◽

pp. 11-15 ◽

Cited By ~ 7

Author(s):

Haydar Ali Erken ◽

Gülten Erken ◽

Osman Genç

Keyword(s):

Blood Pressure ◽

Pressure Measurement ◽

Blood Pressure Measurement ◽

Freely Moving Rats ◽

Freely Moving ◽

Tail Cuff

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His-Leu, an angiotensin I-derived peptide, does not affect haemodynamics in rats

Journal of the Renin-Angiotensin-Aldosterone System ◽

10.1177/1470320318808879 ◽

2018 ◽

Vol 19 (4) ◽

pp. 147032031880887 ◽

Cited By ~ 1

Author(s):

Adrian Drapała ◽

Klaudia Bielińska ◽

Piotr Konopelski ◽

Leszek Pączek ◽

Marcin Ufnal

Keyword(s):

Blood Pressure ◽

Body Weight ◽

Arterial Blood Pressure ◽

Haemodynamic Effects ◽

Hypertensive Rats ◽

Angiotensin I ◽

Arterial Blood ◽

Freely Moving Rats ◽

Significant Difference ◽

Freely Moving

Introduction: The dipeptide histidine-leucine (His-Leu) is formed in the process of converting angiotensin I into angiotensin II. Several studies show that short peptides containing His-Leu may produce significant haemodynamic effects; however, to the best of our knowledge, data on haemodynamic effects of His-Leu are not available in medical databases. Materials and methods: We evaluated acute haemodynamic effects of intravenous administration of either 0.9% NaCl (vehicle) or His-Leu at a dose of 3–15 mg/kg body weight in anaesthetized 15–16-week-old, male, normotensive Wistar Kyoto and spontaneously hypertensive rats. Chronic effects of treatment with either the vehicle or His-Leu at a dose of 15 mg/kg body weight given subcutaneously daily were determined during continuous telemetry recordings in freely moving rats. Results: In anaesthetized rats both the vehicle and His-Leu produced a mild and transient increase in blood pressure and no change in plasma renin activity. There was no significant difference in haemodynamics between the rats infused with the vehicle and the rats infused with His-Leu. In chronic studies, seven-day treatment with vehicle and with His-Leu did not affect arterial blood pressure in freely moving rats. Conclusion: His-Leu does not produce either acute or chronic changes in arterial blood pressure in normotensive and hypertensive rats.

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