Evaluation of a novel radiotracer for positron emission tomography imaging of reactive oxygen species in the central nervous system

2017 ◽  
Vol 53 ◽  
pp. 14-20 ◽  
Author(s):  
Alan A. Wilson ◽  
Oleg Sadovski ◽  
José N. Nobrega ◽  
Roger J. Raymond ◽  
Francis R. Bambico ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Positron Emission Tomography ◽  
Central Nervous System ◽  
Nervous System ◽  
Positron Emission Tomography Imaging ◽  
Emission Tomography ◽  
Oxygen Species ◽  
Tomography Imaging ◽  
Positron Emission ◽  
The Central Nervous System

scholarly journals Positron emission tomography reporter gene strategy for use in the central nervous system

2019 ◽  
Vol 116 (23) ◽  
pp. 11402-11407 ◽  
Author(s):  
Tom Haywood ◽  
Corinne Beinat ◽  
Gayatri Gowrishankar ◽  
Chirag B. Patel ◽  
Israt S. Alam ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Central Nervous System ◽  
Nervous System ◽  
Reporter Gene ◽  
Pet Imaging ◽  
Emission Tomography ◽  
Great Promise ◽  
Adeno Associated Virus ◽  
Positron Emission ◽  
The Central Nervous System

There is a growing need for monitoring or imaging gene therapy in the central nervous system (CNS). This can be achieved with a positron emission tomography (PET) reporter gene strategy. Here we report the development of a PET reporter gene system using the PKM2 gene with its associated radiotracer [18F]DASA-23. The PKM2 reporter gene was delivered to the brains of mice by adeno-associated virus (AAV9) via stereotactic injection. Serial PET imaging was carried out over 8 wk to assess PKM2 expression. After 8 wk, the brains were excised for further mRNA and protein analysis. PET imaging at 8 wk post-AAV delivery showed an increase in [18F]DASA-23 brain uptake in the transduced site of mice injected with the AAV mice over all controls. We believe PKM2 shows great promise as a PET reporter gene and to date is the only example that can be used in all areas of the CNS without breaking the blood–brain barrier, to monitor gene and cell therapy.

Sign in / Sign up

Export Citation Format

Share Document