Abstract. Environmental flow (E-flow) frameworks advocate holistic, regional-scale,
probabilistic E-flow assessments that consider flow and non-flow drivers of
change in a socio-ecological context as best practice. Regional-scale
ecological risk assessments of multiple stressors to social and ecological
endpoints, which address ecosystem dynamism, have been undertaken
internationally at different spatial scales using the relative-risk model
since the mid-1990s. With the recent incorporation of Bayesian belief
networks into the relative-risk model, a robust regional-scale ecological
risk assessment approach is available that can contribute to achieving the
best practice recommendations of E-flow frameworks. PROBFLO is a holistic
E-flow assessment method that incorporates the relative-risk model and
Bayesian belief networks (BN-RRM) into a transparent probabilistic modelling
tool that addresses uncertainty explicitly. PROBFLO has been developed to
evaluate the socio-ecological consequences of historical, current and future
water resource use scenarios and generate E-flow requirements on regional
spatial scales. The approach has been implemented in two regional-scale case
studies in Africa where its flexibility and functionality has been
demonstrated. In both case studies the evidence-based outcomes facilitated
informed environmental management decision making, with trade-off
considerations in the context of social and ecological aspirations. This
paper presents the PROBFLO approach as applied to the Senqu River catchment
in Lesotho and further developments and application in the Mara River
catchment in Kenya and Tanzania. The 10 BN-RRM procedural steps incorporated
in PROBFLO are demonstrated with examples from both case studies. PROBFLO can
contribute to the adaptive management of water resources and contribute to
the allocation of resources for sustainable use of resources and address
protection requirements.