Expression and purification of recombinant cytoplasmic domain of human erythrocyte band 3 with hexahistidine tag or chitin-binding tag in Escherichia coli

2004 ◽  
Vol 34 (2) ◽  
pp. 167-175 ◽  
Author(s):  
Yu Ding ◽  
Weihua Jiang ◽  
Yang Su ◽  
Hanqing Zhou ◽  
Zhihong Zhang
Keyword(s):  
Escherichia Coli ◽  
Human Erythrocyte ◽  
Cytoplasmic Domain ◽  
Band 3 ◽  
Expression And Purification ◽  
Hexahistidine Tag

Further Studies on Zn2+-Mediated Domain–Domain Communication in Human Erythrocyte Band 3

1998 ◽  
Vol 18 (5) ◽  
pp. 265-277
Author(s):  
Hong Xu ◽  
Xujia Zhang ◽  
Fu Yu Yang
Keyword(s):  
Conformational Change ◽  
Human Erythrocyte ◽  
Cytoplasmic Domain ◽  
Band 3 ◽  
Anion Transport ◽  
Intrinsic Fluorescence ◽  
Transport Activity ◽  
Membrane Domain ◽  
Nmr Study ◽  
Anion Transport Activity

Human erythrocyte band 3 is purified and reconstituted into vesicles, forming right-side-out proteoliposomes. Zn2+ entrapped inside the proteoliposomes inhibits the anion transport activity of band 3, and removal of the cytoplasmic domain of band 3 is able to diminish Zn2+ inhibition. Thus, the inhibition of activity of band 3 results from the Zn2+ induced conformational change of the cytoplasmic domain, which in turn is transmitted to the membrane domain. The results of intrinsic fluorescence and its quenching by HB and the 35Cl NMR study indicate that the cytoplasmic domain is essential for the conformational change induced by Zn2+.SH-blocking reagents, CH3I and GSSG, are used to modify the cytoplasmic domain, where they specifically bind to Cys201 and Cys317. It is observed that the Zn2+ induced inhibition of anion transport activity is blocked. This demonstrates that Cys201 and Cys317 are required in Zn2+-mediated domain–domain communication.

Crystallization and preliminary X-ray analysis of the cytoplasmic domain of human erythrocyte band 3

1995 ◽  
Vol 22 (3) ◽  
pp. 293-297 ◽  
Author(s):  
Anatoly B. Kiyatkin ◽  
Padmaja Natarajan ◽  
Sanjeev Munshi ◽  
Wladyslaw Minor ◽  
John E. Johnson ◽  
...  
Keyword(s):  
Human Erythrocyte ◽  
Cytoplasmic Domain ◽  
Band 3 ◽  
X Ray

scholarly journals Expression, purification, and characterization of the functional dimeric cytoplasmic domain of human erythrocyte band 3 inEscherichia coli

1992 ◽  
Vol 1 (9) ◽  
pp. 1206-1214 ◽  
Author(s):  
Cheng C. Wang ◽  
Ryuichi Moriyama ◽  
Philip S. Low ◽  
Janine A. Badylak ◽  
Jack E. Dixon ◽  
...  
Keyword(s):  
Human Erythrocyte ◽  
Cytoplasmic Domain ◽  
Band 3 ◽  
Inescherichia Coli ◽  
Purification And Characterization

scholarly journals Hydrodynamic examination of the dimeric cytoplasmic domain of the human erythrocyte anion transporter, band 3

1996 ◽  
Vol 71 (3) ◽  
pp. 1611-1615 ◽  
Author(s):  
H. Cölfen ◽  
S.E. Harding ◽  
J.M. Boulter ◽  
A. Watts
Keyword(s):  
Human Erythrocyte ◽  
Cytoplasmic Domain ◽  
Band 3 ◽  
Anion Transporter
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