Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems

2013 ◽  
Vol 92 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Susan Zelasko ◽  
Amrita Palaria ◽  
Aditi Das
Keyword(s):  
Escherichia Coli ◽  
Cytochrome P450 ◽  
Expression Systems ◽  
High Level Expression ◽  
Escherichia Coli Expression ◽  
High Level

High-Fidelity Translation of Recombinant Human Hemoglobin in Escherichia coli

1998 ◽  
Vol 64 (5) ◽  
pp. 1589-1593 ◽  
Author(s):  
Michael J. Weickert ◽  
Izydor Apostol
Keyword(s):  
Escherichia Coli ◽  
Error Rates ◽  
High Fidelity ◽  
Human Hemoglobin ◽  
Heterologous Proteins ◽  
Expression Systems ◽  
High Level Expression ◽  
E Coli ◽  
Translation Error ◽  
High Level

ABSTRACT Coexpression of di-α-globin and β-globin in Escherichia coli in the presence of exogenous heme yielded high levels of soluble, functional recombinant human hemoglobin (rHb1.1). High-level expression of rHb1.1 provides a good model for measuring mistranslation in heterologous proteins. rHb1.1 does not contain isoleucine; therefore, any isoleucine present could be attributed to mistranslation, most likely mistranslation of one or more of the 200 codons that differ from an isoleucine codon by 1 bp. Sensitive amino acid analysis of highly purified rHb1.1 typically revealed ≤0.2 mol of isoleucine per mol of hemoglobin. This corresponds to a translation error rate of ≤0.001, which is not different from typical translation error rates found for E. coli proteins. Two different expression systems that resulted in accumulation of globin proteins to levels equivalent to ∼20% of the level of E. colisoluble proteins also resulted in equivalent translational fidelity.

High‐level expression of functional human cytochrome P450 1A2 in Escherichia coli

1992 ◽  
Vol 6 (2) ◽  
pp. 759-764 ◽  
Author(s):  
Charles W. Fisher ◽  
Deborah L. Caudle ◽  
Cheryl Martin‐Wixtrom ◽  
Linda C. Quattrochi ◽  
Robert H. Tukey ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cytochrome P450 ◽  
High Level Expression ◽  
Cytochrome P450 1A2 ◽  
Human Cytochrome ◽  
High Level

Functional High Level Expression of Cytochrome P450 CYP2D6 Using Baculoviral Expression Systems

1996 ◽  
Vol 328 (1) ◽  
pp. 143-150 ◽  
Author(s):  
Mark J.I. Paine ◽  
David Gilham ◽  
Gordon C.K. Roberts ◽  
C.Roland Wolf
Keyword(s):  
Cytochrome P450 ◽  
Expression Systems ◽  
High Level Expression ◽  
High Level

High level expression of human lipocortin (annexin) 1 in Escherichia coli

1996 ◽  
Vol 18 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Kwang-Rae Huh ◽  
Eun Hee Cho ◽  
Soo Ok Lee ◽  
Doe Sun Na
Keyword(s):  
Escherichia Coli ◽  
High Level Expression ◽  
Annexin 1 ◽  
High Level
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