Expression and purification of pneumococcal surface protein a of clade 4 in Escherichia coli using hydroxylapatite and ion-exchange column chromatography

2018 ◽  
Vol 151 ◽  
pp. 56-61 ◽  
Author(s):  
Hualong Xi ◽  
Jinfei Yu ◽  
Qing Sun ◽  
Jingcai Lu ◽  
Tiejun Gu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ion Exchange ◽  
Column Chromatography ◽  
Protein A ◽  
Surface Protein ◽  
Expression And Purification ◽  
Exchange Column ◽  
Pneumococcal Surface Protein A ◽  
Ion Exchange Column

Quantitative Method for Estimating Fecal Amino Acids

1975 ◽  
Vol 21 (10) ◽  
pp. 1437-1440 ◽  
Author(s):  
Christopher W I Owens ◽  
Walfredo Padovan
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Column Chromatography ◽  
Quantitative Method ◽  
Normal Values ◽  
Amino Nitrogen ◽  
Exchange Column ◽  
Ion Exchange Column ◽  
Fresh Stool

Abstract We describe a method for quantitatively estimating 24 ninhydrin-reacting substances, including the commoner amino acids, in fecal dialysate prepared from ingested dialysis bags retrieved from fresh stool. It is accurate to 2 µmol of α-amino nitrogen per liter of fecal dialysate, and for most substances recovery of added standards is 100%. It involves dilution, ultrafiltration, and automated ion-exchange column chromatography of the dialysate. Some normal values are provided.

scholarly journals Production and purification of recombinant fragment of pneumococcal surface protein A (PspA) in Escherichia coli

2011 ◽  
Vol 4 ◽  
pp. 27-35 ◽  
Author(s):  
Giovana C. Barazzone ◽  
Rimenys Carvalho ◽  
Stefanie Kraschowetz ◽  
Antonio L. Horta ◽  
Cíntia R. Sargo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein A ◽  
Surface Protein ◽  
Recombinant Fragment ◽  
Pneumococcal Surface Protein A

Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography

2007 ◽  
Vol 70 (3) ◽  
pp. 493-498 ◽  
Author(s):  
Tsutomu Arakawa ◽  
Kouhei Tsumoto ◽  
Daisuke Ejima ◽  
Yoshiko Kita ◽  
Yasushi Yonezawa ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Ammonium Sulfate ◽  
Column Chromatography ◽  
Exchange Column ◽  
Ion Exchange Column

scholarly journals Fractionnement sur échangeur anionique des colloïdes glucidiques solubles (gommes et pectines) du jus de raisin sain

OENO One ◽  
1980 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Denis Dubourdieu ◽  
Pascal Ribéreau-Gayon
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Galacturonic Acid ◽  
Exchange Column ◽  
Ion Exchange Column ◽  
Neutral Sugars

<p style="text-align: justify;">Les gommes d'un moût de raisins sains sont fractionnés par chromatographie d'échange anionique sur D.E.A.E. Sephadex A 25. On peut ainsi caractériser des gommes neutres qui ne contiennent que des oses neutres dans leur molécule et des gommes acides qui comportent des oses neutres et de l'acide galacturonique.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Gums of juice from healthy grappes are fractionnated by ion exchange column chromatography on D.E.A.E. Sephadex A 25. So are separated neutral gums, containing only neutral sugars in their molecules, from acidic gums, that contains neutral sugars and galacturonic acid.</p>

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