Myrosinase in horseradish (Armoracia rusticana) root: Isolation of a full-length cDNA and its heterologous expression in Spodoptera frugiperda insect cells

Plant Science ◽  
2007 ◽  
Vol 172 (6) ◽  
pp. 1095-1102 ◽  
Author(s):  
Xian Li ◽  
Zhimou Wen ◽  
Hans J. Bohnert ◽  
Mary A. Schuler ◽  
Mosbah M. Kushad
Keyword(s):  
Heterologous Expression ◽  
Spodoptera Frugiperda ◽  
Insect Cells ◽  
Full Length ◽  
Armoracia Rusticana ◽  
Full Length Cdna ◽  
Root Isolation

scholarly journals (275) Cloning of a Myrosinase cDNA from Horseradish (Armoracia rusticana) Root and Its Heterologous Expression in Spodoptera frugiperda Insect Cells

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1021B-1021
Author(s):  
Xian Li ◽  
Hans Bohnert ◽  
Mary Schuler ◽  
Mosbah Kushad
Keyword(s):  
Heterologous Expression ◽  
Spodoptera Frugiperda ◽  
Insect Cells ◽  
Expression Patterns ◽  
Amino Acid Identity ◽  
Sequence Information ◽  
Apparent Mass ◽  
Armoracia Rusticana ◽  
Reading Frame ◽  
Associated Proteins

A full-length cDNA (ArMY1) encoding myrosinase (β-thioglucoside glucohydrolase, EC 3.2.3.1) was cloned from horseradish (Armoracia rusticana) root. ArMY1 has an open reading frame of 1614 nucleotides with a deduced protein of 538 amino acids and molecular mass of 61.6 kD. ArMY1 shows highest overall amino acid identity (72%) with Arabidopsis thaliana myrosinase TGG2. ArMY1 mRNA signal of about 1.95 kb was detected in the leaves and roots of horseradish, but not in the leaves of broccoli. Heterologous expression of ArMY1 in baculovirus-infected Sf9 cells resulted in an immunologically active recombinant ArMY1 protein when probed with myrosinase-specific monoclonal antibody 3D7 with apparent mass 65 kD. Phylogenetic analysis showed that ArMY1 does not cluster with any of the current myrosinase subfamilies, i.e., the MA, MB, and MC subfamilies, and may represent a novel myrosinase subfamily in root tissue. This is the first report of cloning of myrosinase cDNA from horseradish root. It provides important sequence information that will enable further studies of myrosinase expression patterns and their interaction with myrosinase–binding proteins and other myrosinase-associated proteins.

Generation of Arabidopsis Mutants by Heterologous Expression of a Full-Length cDNA Library from Tomato Fruits

2009 ◽  
Vol 27 (4) ◽  
pp. 454-461 ◽  
Author(s):  
Yi-Hong Wang ◽  
Chris M. Mosebach ◽  
Abraham S. Kibbey ◽  
Marcie K. Ryhal ◽  
Angelica D. Jones ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Cdna Library ◽  
Full Length ◽  
Tomato Fruits ◽  
Arabidopsis Mutants ◽  
Full Length Cdna

Establishment and Identification of a Normalized Full-Length cDNA Library of CCRI 36

2009 ◽  
Vol 35 (4) ◽  
pp. 602-607 ◽  
Author(s):  
Dong WU ◽  
Jun-Jie LIU ◽  
Shu-Xun YU ◽  
Shu-Li FAN ◽  
Mei-Zhen SONG
Keyword(s):  
Cdna Library ◽  
Full Length ◽  
Full Length Cdna

Full length cDNA cloning and tissue expression of PRLR in Cyprinus carpio var. jian

2013 ◽  
Vol 20 (4) ◽  
pp. 722-732
Author(s):  
Bing LI ◽  
Jie WANG ◽  
Chengfeng ZHANG ◽  
Jian ZHU
Keyword(s):  
Cdna Cloning ◽  
Cyprinus Carpio ◽  
Tissue Expression ◽  
Full Length ◽  
Full Length Cdna

scholarly journals UNAGI: an automated pipeline for nanopore full-length cDNA sequencing uncovers novel transcripts and isoforms in yeast

2020 ◽  
Vol 20 (4) ◽  
pp. 523-536
Author(s):  
Mohamad Al kadi ◽  
Nicolas Jung ◽  
Shingo Ito ◽  
Shoichiro Kameoka ◽  
Takashi Hishida ◽  
...  
Keyword(s):  
Full Length ◽  
Cdna Sequencing ◽  
Full Length Cdna ◽  
Novel Transcripts ◽  
Automated Pipeline
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