Persistence Enhancement of a Promising Tick Repellent, Benzyl Isothiocyanate, by Yeast Microcarriers

Phenethyl isothiocyanate isolated from Armoracia rusticana root oil and its derivatives were tested at different doses in a bioassay designed to evaluate repellency against individual Haemaphysalis longicornis nymphs. Among the tested compounds, benzyl isothiocyanate exhibited repellency against H. longicornis nymphs at the lowest dose of 0.00625 mg/cm2, followed by phenethyl isothiocyanate (0.0125 mg/cm2) and phenyl isothiocyanate (0.025 mg/cm2). The behavioral responses of H. longicornis nymphs exposed to benzyl isothiocyanate and phenethyl isothiocyanate indicated that the mode of action of these compounds can be mainly attributed to the vapor phase. Encapsulated benzyl isothiocyanate showed repellency up to 120 min post-application at 0.1 mg/cm2, whereas pure benzyl isothiocyanate showed repellency up to 60 min post-application at 0.1 mg/cm2. The present study suggests that benzyl isothiocyanate is a potential repellent for protection against H. longicornis nymphs, and encapsulation in yeast cells may enhance the repellency effect.

White Rust Albugo candida (Pers.) Kuntze, 1797 in Horseradish Crops (Armoracia rusticana G. Gaertn., B. Mey. and Scherb., 1800) in the South of the Far East and Measures to Control it

Background: Horseradish is a valuable agricultural crop with gastronomic and medical significance. It contains mono- and polysaccharides, protein compounds and organic acids, minerals and starch, as well as a rich vitamin complex. Unfortunately, horseradish diseases are still insufficiently studied, while crop losses from them can be quite significant. The purpose of our study was to determine the composition of pathogenic fungi on horseradish Armoracia rusticana G. Gaertn., B.Mey. and Scherb. and to develop measures to combat them. Methods: The research was carried out on horseradish “Atlant” varieties in the plantings of the of FSBSI “Far East Federal Research Center of agrobiotechnology n.a. A.K. Chaika” at 2019-2020. Studies of phytopathogens were carried out in the field conditions and in vitro. Microscopy was performed using Levenhuk D740T, 5,1 MP. Lifelong injuries photofixation was performed using Sony SAL1855. Processing of the microscopy results was carried out using Outfi, PluriIQ, CellProfiler software. In the experiment to study the agent fungicidal efficacy against horseradish diseases, we used: Acrobat WG, Bravo SC, Zummer SC, Consento SC, Infinito SC, Topaz EW, Ordan SP, Thiovit Jet WG, Rajok EW, Proton Extra RG, Ridomil Gold MZ RG. The agents were diluted in doses of 10%, 30% and 70% of the recommended dose, the index of conidia occurrence was calculated, phytotoxicity was carried out. Result: For five iterations, the greatest fungicidal activity was demonstrated by the Acrobat WG, Topaz EW and Proton Extra WG agents. The maximum effectiveness was observed for the agent Ridomil Gold MZ WG. The lowest efficiency was shown by the Infinito SC, Consento SC, Ordan SP, Thiovit Jet WG agents. No phytotoxic effect was found in any of the agents.

THE IMPACT OF HORSERADISH ROOTS ($ARMORACIA$ $RUSTICANA$) ON THE FUNCTIONAL STATE OF RAT BODY IN ACUTE HYPOXIA

Using the “Bioscope” hardware complex, it was shown that an oxygen deficiency leads to certain changes in the statistical parameters of the integrative state of animals both during and after hypoxia exposure. However, after 10 days of using horseradish roots (Armoracia rusticana) as an antihypoxic food supplement, in contrast to the control, significant changes in the recorded signal are observed both during and after hypoxic exposure. These changes indicate the facilitating effect of horseradish roots in conditions of oxygen deficiency.

Synthesis of Divanillin in the Presence of Water Extract and Juice of Horseradish Root

The divanillin synthesis by oxidative dimerization of vanillin in the presence of juice and water extracts of horseradish root (Armorácia rusticána), a more available and cheaper catalyst as compared with horseradish reactive peroxidase (HRP) was studied. The divanillin yield exceeds 90 mol. % based on loaded vanillin. The peroxidase activity in juice and water extracts of horseradish root was estimated

Volatile Organic Compounds (VOCs) of Endophytic Fungi Growing on Extracts of the Host, Horseradish (Armoracia rusticana)

The interaction between plant defensive metabolites and different plant-associated fungal species is of high interest to many disciplines. Volatile organic compounds (VOCs) are natural products that are easily evaporated under ambient conditions. They play a very important role in inter-species communication of microbes and their hosts. In this study, the VOCs produced by 43 different fungal isolates of endophytic and soil fungi during growth on horseradish root (Armoracia rusticana) extract or malt extract agar were examined, by using headspace-gas chromatography-mass spectrometry (headspace-GC-MS) and a high relative surface agar film as a medium. The proposed technique enabled sensitive detection of several typical VOCs (acetone, methyl acetate, methyl formate, ethyl acetate, methyl butanol isomers, styrene, beta-phellandrene), along with glucosinolate decomposition products, including allyl cyanide and allyl isothiocyanate and other sulfur-containing compounds—carbon disulfide, dimethyl sulfide. The VOC patterns of fungi belonging to Setophoma, Paraphoma, Plectosphaerella, Pyrenochaeta, Volutella, Cadophora, Notophoma, and Curvularia genera were described for the first time. The VOC pattern was significantly different among the isolates. The pattern was indicative of putative myrosinase activity for many tested isolates. On the other hand, endophytes and soil fungi as groups could not be separated by VOC pattern or intensity.

Proteomic analysis of exudate of Cercospora armoraciae from Armoracia rusticana

Background Cercospora armoraciae causes leaf spot disease on Armoracia rusticana. Exudation of droplets, when grown on PDA, distinguishes this fungi from other members of the genus Cercospora. The role this exudate plays in the virulence of this pathogen has not been elucidated. To explore this, we characterized the transcriptome of C. armoraciae and the proteome of exudate associated with this plant pathogen. Methods Virulence of three strains of C. armoraciae was evaluated in greenhouse assays. De novo sequencing was applied to assemble transcriptome from these strains. Nano-HPLC-MS/MS analysis was used to identify proteins in the pathogen exudate. Identified proteins were functionally classified and annotated using GO, KEGG, and COG/KOG bioinformatics analysis methods. Results When treated with the exudate of C. armoraciae strain SCa-01, leaves of A. rusticana showed yellowing and necrosis of the leaves and similar symptoms to plants inoculated with this fungi. A total of 14,937 unigenes were assembled from C. armoraciae, and 576 proteins comprising 1,538 peptides, 1,524 unique peptide, were identified from the exudate. GO annotation classified 411 proteins (71%) into 27 functional categories, namely, 12, seven and eight biological process, cellular component, and molecular function subcategories, respectively. KEGG analysis assigned 314 proteins to 84 signaling/metabolic pathways, and 450 proteins were annotated against the COG/KOG database. Discussion Transcriptome and GO analysis of C. armoraciae found most proteins in the exudate. GO analysis suggested that a considerable proportion of proteins were involved in cellular process and metabolic process, which suggests exudates maintain the metabolic balance of this fungi. Some proteins annotated to the phenylalanine metabolism, which suggests that the exudates may enhance the virulence of this pathogen. Some proteins annotated to the phenylalanine metabolism, which suggests that the exudates may enhance the pathogenicity of the pathogen. Also some proteins were annotated to the peroxisome metabolic pathway and the fatty acid biosynthesis pathways. These pathways may confer antifungal, antioxidant and antimicrobial activity on the exudates.

Bacterial endophytes from horseradish (Armoracia rusticana G. Gaertn., B. Mey. & Scherb.) with antimicrobial efficacy against pathogens

The current study aimed to determine the diversity of culturable endophytic bacteria associated with horseradish (Armoracia rusticana G. Gaertn., B. Mey. & Scherb.) grown in Chatkal Biosphere Reserve of Uzbekistan and their antimicrobial potentials. The bacteria were isolated from plant leaves and root tissues using culture-dependent techniques. The 16S rRNA sequences similarities of endophytic bacteria isolated from A. rusticana showed that isolates belong to species Paenibacillus, Raoultella, Stenotrophomonas, Pseudomonas, Serratia, Microbacterium, Enterobacter, Achromobacter, Brevibacterium, Pantoea, and Erwinia. The isolated endophytic bacteria Stenotrophomonas maltophilia KRT1, Serratia ficaria KRT5, and Pantoea agglomerans KLT4 possess antimicrobial activities against human pathogenic bacteria Staphylococcus aureus, Escherichia coli, and Candida albicans. The endophytic bacteria Paenibacillus typhae KRN1, Stenotrophomonas maltophilia KRT1, Pseudomonas kilonensis KRT11, Pseudomonas umsongensis KRT21, Brevibacterium frigoritolerans KLT2 and Pantoea agglomerans KLT4 inhibited phytopathogenic fungi Rhizoctonia solani, Fusarium culmorum, and F. solani. These findings indicate that plant endophytic bacteria with antimicrobial activity could be a source for producing agriculturally and pharmaceutically important antimicrobial compounds.

Hydro-alcoholic Extracts of Roots and Sprouts of Armoracia rusticana: Assessment of Antioxidant and Cytotoxic Activity

Armoracia rusticana � horseradish is a well-known vegetable used from ancient times and consumed nowadays more for its taste than its pharmacological properties, even though it proved to be a good agent in fighting several health problems. This study focuses on a comparative assessment of several horseradish hydro-alcoholic extracts, obtained from roots and sprouts, respectively, mainly evaluating the antioxidant activity but also a potential cytotoxic effect. The DPPH evaluation revealed that horseradish extracts made from roots or sprouts exhibit a strong antioxidant activity compared to the ascorbic acid used as etalon. The in vitro evaluation established that a significant inhibitory effect on cell viability and morphology of MDA-MB-231 � breast adenocarcinoma cell line was displayed by the hydroalcoholic extracts in a dose-dependent manner.

Phytochemical Analysis and Antidiabetic Potential of Armoracia Rusticana: Pharmacological and Computational Approach

Aims & Objective: Armoracia Rusticana has high medicinal values and is an excellent source of phytochemicals. This study was aimed to evaluate the antidiabetic potential of bioactive compounds from Armoracia Rusticana. Methods: The antidiabetic analysis revealed Armoracia Rusticana was highly active against α-glucosidase with IC50 values of 5.6 µg/ml. Furthermore, molecular docking was used to identify the active constituents against α-glucosidase, while using acarbose as a controlled drug. Results: Upon phytochemical screening, it was found that six out of ten phytochemicals were successfully docked in the respective binding sites. The lead phytochemical was Quercetin 3-O-beta-D-xylopyranoside which displayed a more binding score as compared to acarbose. They were subjected to analyze for drug-like properties which further strengthen its validation. Conclusion: It was, therefore, concluded that Armoracia Rusticana might potentially be used in the amelioration of type 2 diabetes. Potential molecules identified from this study could be considered as a lead drug to cure diabetes mellitus.