CRISPR-based knock-out of eIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus

Plant Science ◽  
2021 ◽  
pp. 111160
Author(s):  
Kyoka Kuroiwa ◽  
Christina Thenault ◽  
Fabien Nogué ◽  
Laura Perrot ◽  
Marianne Mazier ◽  
...  
Keyword(s):  
Mottle Virus ◽  
Cherry Tomato ◽  
Knock Out ◽  
Pepper Veinal Mottle Virus

scholarly journals Knock-out mutation of eukaryotic initiation factor 4E2 (eIF4E2) confers resistance to pepper veinal mottle virus in tomato

Virology ◽  
2020 ◽  
Vol 539 ◽  
pp. 11-17 ◽  
Author(s):  
Benoît Moury ◽  
Caroline Lebaron ◽  
Marion Szadkowski ◽  
Mekki Ben Khalifa ◽  
Grégory Girardot ◽  
...  
Keyword(s):  
Initiation Factor ◽  
Mottle Virus ◽  
Eukaryotic Initiation Factor ◽  
Knock Out ◽  
Pepper Veinal Mottle Virus

scholarly journals Use of an Infectious cDNA Clone of Pepper Veinal Mottle Virus to Confirm the Etiology of a Disease in Capsicum chinense

2020 ◽  
Vol 110 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Weiyao Hu ◽  
Li Qin ◽  
Haixia Yan ◽  
Weiguo Miao ◽  
Hongguang Cui ◽  
...  
Keyword(s):  
Cdna Clone ◽  
Hainan Island ◽  
Mottle Virus ◽  
Reverse Genetic ◽  
Capsicum Chinense ◽  
Genetic Tool ◽  
Pepper Veinal Mottle Virus ◽  
Virus Progeny ◽  
Local Germplasm ◽  
Pepper Cultivar

The pepper cultivar Yellow Lantern, one of the spiciest pepper varieties, is a local germplasm of Capsicum chinense, cultivated exclusively on Hainan Island, China. However, this variety is susceptible to viral diseases that severely affect its production. In this study, we report that pepper veinal mottle virus (PVMV) is associated with foliar chlorosis and rugosity symptoms in Yellow Lantern. To verify this correlation, we constructed a full-length cDNA clone of a PVMV isolate named HNu. The virus progeny derived from the cDNA clone replicated and moved systemically in the pepper, inducing the same symptoms as those induced by PVMV-HNu in Yellow Lantern peppers in the field. The results support that PVMV-HNu is the causal agent of foliar chlorosis and rugosity disease in Yellow Lantern. This knowledge will help in the diagnosis and prevention of disease caused by PVMV. Furthermore, the cDNA clone serves as a reverse genetic tool to study the molecular pathogenesis of PVMV.

scholarly journals Serological, Molecular, and Pathotype Diversity of Pepper veinal mottle virus and Chili veinal mottle virus

2005 ◽  
Vol 95 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Benoît Moury ◽  
Alain Palloix ◽  
Carole Caranta ◽  
Patrick Gognalons ◽  
Sylvie Souche ◽  
...  
Keyword(s):  
Central Africa ◽  
Field Trials ◽  
Mottle Virus ◽  
Chain Reactions ◽  
Resistance Factors ◽  
Polymerase Chain Reactions ◽  
Cp Gene ◽  
Pepper Veinal Mottle Virus ◽  
Recessive Genes ◽  
Chili Veinal Mottle Virus

Variability within the pepper-infecting potyviruses Pepper veinal mottle virus (PVMV) and Chili veinal mottle virus (ChiVMV) in Africa and Asia was investigated. Coat protein (CP) gene sequence diversity revealed three clades that corresponded to three geographic locations and there was no evidence of presence of the ChiVMV/Asian group in western or central Africa. These clades included closely related isolates that potentially belong to two viral species, which is consistent with current nomenclature. These clades could not be unambiguously identified with polyclonal antisera; however, reverse transcription-polymerase chain reactions allowed differentiation of the isolates into two species based on a large indel in the CP gene. PVMV and ChiVMV isolates were classified into three and two pathotypes, respectively, in relation to pepper genotypes carrying different resistance factors. Specificity of resistance only partially corresponded to molecular diversity of the isolates. Only one isolate of PVMV could infect pepper genotypes carrying the two recessive genes pvr6 and pvr2 2; however, these genotypes were not infected by PVMV in field trials in Senegal, despite a high prevalence of PVMV in the surrounding pepper plants.

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