scholarly journals Knock-out mutation of eukaryotic initiation factor 4E2 (eIF4E2) confers resistance to pepper veinal mottle virus in tomato

Virology ◽  
2020 ◽  
Vol 539 ◽  
pp. 11-17 ◽  
Author(s):  
Benoît Moury ◽  
Caroline Lebaron ◽  
Marion Szadkowski ◽  
Mekki Ben Khalifa ◽  
Grégory Girardot ◽  
...  
Keyword(s):  
Initiation Factor ◽  
Mottle Virus ◽  
Eukaryotic Initiation Factor ◽  
Knock Out ◽  
Pepper Veinal Mottle Virus

CRISPR-based knock-out of eIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus

Plant Science ◽  
2021 ◽  
pp. 111160
Author(s):  
Kyoka Kuroiwa ◽  
Christina Thenault ◽  
Fabien Nogué ◽  
Laura Perrot ◽  
Marianne Mazier ◽  
...  
Keyword(s):  
Mottle Virus ◽  
Cherry Tomato ◽  
Knock Out ◽  
Pepper Veinal Mottle Virus

scholarly journals Simultaneous mutations in translation initiation factors eIF4E and eIF(iso)4E are required to prevent pepper veinal mottle virus infection of pepper

2006 ◽  
Vol 87 (7) ◽  
pp. 2089-2098 ◽  
Author(s):  
Sandrine Ruffel ◽  
Jean-Luc Gallois ◽  
Benoît Moury ◽  
Christophe Robaglia ◽  
Alain Palloix ◽  
...  
Keyword(s):  
Transient Expression ◽  
Translation Initiation ◽  
Genomic Region ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Mottle Virus ◽  
Susceptibility Allele ◽  
Eukaryotic Translation ◽  
Pepper Veinal Mottle Virus ◽  
Loss Of Resistance

Capsicum resistance to Pepper veinal mottle virus (PVMV) results from complementation between the pvr2 and pvr6 resistance genes: recessive alleles at these two loci are necessary for resistance, whereas any dominant allele confers susceptibility. In line with previous results showing that pvr2 resistance alleles encode mutated versions of the eukaryotic translation initiation factor 4E (eIF4E), the involvement of other members of the eIF4E multigenic family in PVMV resistance was investigated. It was demonstrated that pvr6 corresponds to an eIF(iso)4E gene, predicted to encode the second cap-binding isoform identified in plants. Comparative genetic mapping in pepper and tomato indicated that eIF(iso)4E maps in the same genomic region as pvr6. Sequence analysis revealed an 82 nt deletion in eIF(iso)4E cDNAs from genotypes with the pvr6 resistance allele, leading to a truncated protein. This deletion was shown to co-segregate with pvr6 in doubled haploid and F2 progeny. Transient expression in a PVMV-resistant genotype of eIF(iso)4E derived from a genotype with the pvr6 + susceptibility allele resulted in loss of resistance to subsequent PVMV inoculation, confirming that pvr6 encodes the translation factor eIF(iso)4E. Similarly, transient expression of eIF4E from a genotype with the pvr2 + -eIF4E susceptibility allele also resulted in loss of resistance, demonstrating that wild-type eIF4E and eIF(iso)4E are susceptibility factors for PVMV and that resistance results from the combined effect of mutations in the two cap-binding isoforms. Thus, whilst most potyviruses specifically require one eIF4E isoform to perform their replication cycle, PVMV uses either eIF4E or eIF(iso)4E for infection of pepper.

scholarly journals Allele mining in the pepper gene pool provided new complementation effects between pvr2-eIF4E and pvr6-eIF(iso)4E alleles for resistance to pepper veinal mottle virus

2009 ◽  
Vol 90 (11) ◽  
pp. 2808-2814 ◽  
Author(s):  
Manuel Rubio ◽  
Maryse Nicolaï ◽  
Carole Caranta ◽  
Alain Palloix
Keyword(s):  
Amino Acid ◽  
Gene Pool ◽  
Direct Interaction ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Mottle Virus ◽  
Recessive Resistance ◽  
Resistant Allele ◽  
Pepper Veinal Mottle Virus ◽  
Infection Resistance

Molecular cloning of recessive resistance genes to potyviruses in a large range of host species identified the eukaryotic translation initiation factor 4E (eIF4E) as an essential determinant in the outcome of potyvirus infection. Resistance results from a few amino acid changes in the eIF4E protein encoded by the recessive resistance allele that disrupt the direct interaction with the potyviral protein VPg. In plants, several loci encode two protein subfamilies, eIF4E and eIF(iso)4E. While most eIF4E-mediated resistance to potyviruses depends on mutations in a single eIF4E protein, simultaneous mutations in eIF4E (corresponding to the pvr2 locus) and eIF(iso)4E (corresponding to the pvr6 locus) are required to prevent pepper veinal mottle virus (PVMV) infection in pepper. We used this model to look for additional alleles at the pvr2-eIF4E locus that result in resistance when combined with the pvr6-eIF(iso)4E resistant allele. Among the 12 pvr2-eIF4E resistance alleles sequenced in the pepper gene pool, three were shown to have a complementary effect with pvr6-eIF(iso)4E for resistance. Two amino acid changes were exclusively shared by these three alleles and were systematically associated with a second amino acid change, suggesting that these substitutions are associated with resistance expression. The availability of new resistant allele combinations increases the possibility for the durable deployment of resistance against this pepper virus which is prevalent in Africa.

scholarly journals OseIF3h Regulates Plant Growth and Pollen Development at Translational Level Presumably through Interaction with OsMTA2

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1101
Author(s):  
Yuqing Huang ◽  
Peng Zheng ◽  
Xuejiao Liu ◽  
Hao Chen ◽  
Jumin Tu
Keyword(s):  
Plant Growth ◽  
Translation Initiation ◽  
Pollen Development ◽  
Messenger Rna ◽  
Protein Biosynthesis ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Sequencing Data ◽  
Knock Out ◽  
Translational Level

The initiation stage of protein biosynthesis is a sophisticated process tightly regulated by numerous initiation factors and their associated components. However, the mechanism underlying translation initiation has not been completely understood in rice. Here, we showed knock-out mutation of the rice eukaryotic translation initiation factor 3 subunit h (OseIF3h) resulted in plant growth retardation and seed-setting rate reduction as compared to the wild type. Further investigation demonstrated an interaction between OseIF3h and OsMTA2 (mRNA adenosine methylase 2), a rice homolog of METTL3 (methyltransferase-like 3) in mammals, which provided new insight into how N6-methyladenosine (m6A) modification of messenger RNA (mRNA) is engaged in the translation initiation process in monocot species. Moreover, the RIP-seq (RNA immunoprecipitation sequencing) data suggested that OseIF3h was involved in multiple biological processes, including photosynthesis, cellular metabolic process, precursor metabolites, and energy generation. Therefore, we infer that OseIF3h interacts with OsMTA2 to target a particular subset of genes at translational level, regulating plant growth and pollen development.

Sign in / Sign up

Export Citation Format

Share Document