Enzymatic characterization of Chlamydomonas reinhardtii glycolate dehydrogenase and its nearest proteobacterial homologue

2014 ◽  
Vol 79 ◽  
pp. 25-30 ◽  
Author(s):  
Mohamed H. Aboelmy ◽  
Christoph Peterhansel
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Enzymatic Characterization ◽  
Glycolate Dehydrogenase

Mutants of Chlamydomonas reinhardtii Deficient in Mitochondrial Complex I: Characterization of Two Mutations Affecting the nd1 Coding Sequence

Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 1051-1060
Author(s):  
Claire Remacle ◽  
Denis Baurain ◽  
Pierre Cardol ◽  
René F Matagne
Keyword(s):  
Mitochondrial Genome ◽  
Chlamydomonas Reinhardtii ◽  
Complex I ◽  
Slow Growth ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Sequencing Analysis ◽  
Mitochondrial Complex ◽  
Genetical Analysis ◽  
Complex I Activity

Abstract The mitochondrial rotenone-sensitive NADH:ubiquinone oxidoreductase (complex I) comprises more than 30 subunits, the majority of which are encoded by the nucleus. In Chlamydomonas reinhardtii, only five components of complex I are coded for by mitochondrial genes. Three mutants deprived of complex I activity and displaying slow growth in the dark were isolated after mutagenic treatment with acriflavine. A genetical analysis demonstrated that two mutations (dum20 and dum25) affect the mitochondrial genome whereas the third mutation (dn26) is of nuclear origin. Recombinational analyses showed that dum20 and dum25 are closely linked on the genetic map of the mitochondrial genome and could affect the nd1 gene. A sequencing analysis confirmed this conclusion: dum20 is a deletion of one T at codon 243 of nd1; dum25 corresponds to a 6-bp deletion that eliminates two amino acids located in a very conserved hydrophilic segment of the protein.

scholarly journals Enzymatic characterization of interferon-induced antiviral GTPases murine Mx1 and human MxA proteins.

1994 ◽  
Vol 269 (3) ◽  
pp. 2009-2015 ◽  
Author(s):  
K. Melén ◽  
T. Ronni ◽  
T. Lotta ◽  
I. Julkunen
Keyword(s):  
Enzymatic Characterization

scholarly journals Enzymatic Characterization of the N-Acetylation of 3-Phosphoglyceraldehyde Dehydrogenase by Acetyl Phosphate

1970 ◽  
Vol 245 (11) ◽  
pp. 2946-2953
Author(s):  
Jane Harting Park ◽  
Denis C. Shaw ◽  
Elizabeth Mathew ◽  
Blanche P. Meriwether
Keyword(s):  
Acetyl Phosphate ◽  
Enzymatic Characterization
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