Biochemical characterization, cloning and molecular modeling of a detergent and organic solvent-stable family 11 xylanase from the newly isolated Aspergillus niger US368 strain

2012 ◽  
Vol 47 (12) ◽  
pp. 1839-1847 ◽  
Author(s):  
Aïda Hmida-Sayari ◽  
Salma Taktek ◽  
Fatma Elgharbi ◽  
Samir Bejar
Keyword(s):  
Molecular Modeling ◽  
Aspergillus Niger ◽  
Organic Solvent ◽  
Biochemical Characterization ◽  
Stable Family ◽  
Solvent Stable

Biochemical characterization of xylanase GH11 isolated from Aspergillus niger BCC14405 (XylB) and its application in xylooligosaccharide production

2021 ◽  
Author(s):  
Katesuda Aiewviriyasakul ◽  
Benjarat Bunterngsook ◽  
Hataikarn Lekakarn ◽  
Wipawee Sritusnee ◽  
Pattanop Kanokratana ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Biochemical Characterization

scholarly journals Enhancement of the aspartame precursor synthetic activity of an organic solvent-stable protease

2010 ◽  
Vol 23 (3) ◽  
pp. 147-152 ◽  
Author(s):  
H. Ogino ◽  
S. Tsuchiyama ◽  
M. Yasuda ◽  
N. Doukyu
Keyword(s):  
Organic Solvent ◽  
Synthetic Activity ◽  
Solvent Stable

scholarly journals Biochemical characterization of a glycoside hydrolase family 43 β-D-galactofuranosidase from the fungus Aspergillus niger

2021 ◽  
Author(s):  
Gregory S Bulmer ◽  
Fang Wei Yuen ◽  
Naimah Begum ◽  
Bethan S Jones ◽  
Sabine S Flitsch ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Glycoside Hydrolase ◽  
Biochemical Characterization ◽  
Maximum Activity ◽  
Glycoside Hydrolase Family ◽  
Enzyme Specificity ◽  
Fungal Enzymes ◽  
Glycoside Hydrolase Family 43 ◽  
Hydrolase Family

β-D-Galactofuranose (Galf) and its polysaccharides are found in bacteria, fungi and protozoa but do not occur in mammalian tissues, and thus represent a specific target for anti-pathogenic drugs. Understanding the enzymatic degradation of these polysaccharides is therefore of great interest, but the identity of fungal enzymes with exclusively galactofuranosidase activity has so far remained elusive. Here we describe the identification and characterization of a galactofuranosidase from the industrially important fungus Aspergillus niger. Phylogenetic analysis of glycoside hydrolase family 43 subfamily 34 (GH43_34) members revealed the occurrence of three distinct clusters and, by comparison with specificities of characterized bacterial members, suggested a basis for prediction of enzyme specificity. Using this rationale, in tandem with molecular docking, we identified a putative β-D-galactofuranosidase from A. niger which was recombinantly expressed in Escherichia coli. The Galf-specific hydrolase, encoded by xynD demonstrates maximum activity at pH 5, 25 °C towards 4-Nitrophenyl-β-galactofuranoside (pNP-βGalf), with a Km of 17.9 ± 1.9 mM and Vmax of 70.6 ± 5.3 μmol min-1. The characterization of this first fungal GH43 galactofuranosidase offers further molecular insight into the degradation of Galf-containing structures and may inform clinical treatments against fungal pathogens.

Efficient chemo-enzymatic synthesis of endomorphin-1 using organic solvent stable proteases to green the synthesis of the peptide

2011 ◽  
Vol 13 (7) ◽  
pp. 1680 ◽  
Author(s):  
Honglin Sun ◽  
Bingfang He ◽  
Jiaxing Xu ◽  
Bin Wu ◽  
Pingkai Ouyang
Keyword(s):  
Organic Solvent ◽  
Enzymatic Synthesis ◽  
Solvent Stable

Purification and biochemical characterization of a novel thermostable lichenase from Aspergillus niger US368

2013 ◽  
Vol 98 (1) ◽  
pp. 967-975 ◽  
Author(s):  
Fatma Elgharbi ◽  
Aïda Hmida-Sayari ◽  
Mouna Sahnoun ◽  
Radhouane Kammoun ◽  
Lobna Jlaeil ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Biochemical Characterization

An Organic Solvent-tolerant Bacterium and Its Organic Solvent-stable Protease

1996 ◽  
Vol 799 (1 Enzyme Engine) ◽  
pp. 311-317 ◽  
Author(s):  
HIROYASU OGINO ◽  
KIYOSHI YASUI ◽  
FUMITAKE WATANABE ◽  
HARUO ISHIKAWA
Keyword(s):  
Organic Solvent ◽  
Organic Solvent Tolerant ◽  
Solvent Tolerant ◽  
Solvent Stable
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