<p class="zhengwen"><span lang="EN-GB">Banana peel</span><span lang="IN">s</span><span lang="EN-GB"> is a waste </span><span lang="IN">which</span><span lang="EN-GB"> has not been widely used, so that the relatively long time the existence of such waste brings its own problems such as pollution. Banana peels contain high enough lignocellulosic and can be degraded into simpler forms.</span><span lang="IN"> The</span><span lang="EN-GB"> Lignin content </span><span lang="IN">of</span><span lang="EN-GB"> banana </span><span lang="IN">peels</span><span lang="EN-GB"> needs to be removed / destroyed structure. The purpose of this study was to determine the effect of the addition ratio of <em>Trichoderma reseei </em>and <em>Aspergillus niger</em> on hydrolysis liquefaction and saccharification time in stage hydrolysis to glucose produced with the highest glucose parameters. In this study, the method</span><span lang="IN"> was</span><span lang="EN-GB"> used to degrade lignin </span><span lang="IN">by using sulfuric acid</span><span lang="EN-GB"> (2%). After</span><span lang="IN">ward,</span><span lang="IN"> </span><span lang="EN-GB">the</span><span lang="IN"> cellulose</span><span lang="EN-GB"> content </span><span lang="IN">was changed in </span><span lang="EN-GB">liquefaction </span><span lang="IN">process</span><span lang="EN-GB">. Hydrolysis liquefaction performed using <em>Aspergillus niger</em> and <em>Trichoderma reesei</em> with a mixture ratio of 1: 0, 0: 1, 1: 1 and 1: 2. Furthermore, substrate hydrolysis liquefaction saccharification going through the process of hydrolysis to increase glucose levels were formed. Hydrolysis liquefaction aims to change the content of starch in the banana skin into glucose using Saccharomyces cerevisiae as the addition of 20% (v / v). Hydrolysis process of liquefaction is done by varying the sampling time on day - 3, 6, and 9. In this study the hydrolysis of liquefaction of banana peels use a mixture of <em>Trichoderma reesei</em> and <em>Aspergillus niger</em> 2:1 at a temperature of 50<sup>o</sup>C, pH 5, and 64 h for resulted in glucose with the best content was 0.52%. In addition, the results showed that the hydrolysis saccharification with the addition of <em>Saccharomyces cerevisiae</em> glucose levels were highest on day 3 with a temperature of 30<sup>o</sup>C and pH 5, resulting in glucose content to 1.63%.</span></p>
Laboratory evolution for forced glucose-xylose co-consumption enables identification of mutations that improve mixed-sugar fermentation by xylose-fermenting Saccharomyces cerevisiae