scholarly journals Report of two events of nosocomial outbreak and pseudo-outbreak due to contamination with Achromobacter spp.

2022 ◽  
Author(s):  
Liliana Clara ◽  
María Ines Staneloni ◽  
Estela Salazar ◽  
Graciela Greco ◽  
Mariangeles Visus ◽  
...  
Keyword(s):  
Nosocomial Outbreak

Nosocomial outbreak of scabies

1978 ◽  
Vol 114 (6) ◽  
pp. 897-898 ◽  
Author(s):  
J. J. Gooch
Keyword(s):  
Nosocomial Outbreak

Usefulness of nested PCR and sequence analysis in a nosocomial outbreak of neonatal enterovirus infection

1998 ◽  
Vol 11 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Takeshi Takami ◽  
Hisashi Kawashima ◽  
Yukito Takei ◽  
Tasuku Miyajima ◽  
Takayuki Mori ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nested Pcr ◽  
Nosocomial Outbreak ◽  
Enterovirus Infection

scholarly journals Amplified fragment length polymorphism and whole genome sequencing: a comparison of methods in the investigation of a nosocomial outbreak with vancomycin resistant enterococci

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Victoria A. Janes ◽  
Daan W. Notermans ◽  
Ingrid J.B. Spijkerman ◽  
Caroline E. Visser ◽  
Marja E. Jakobs ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Whole Genome ◽  
Nosocomial Outbreak ◽  
Outbreak Management ◽  
Sequencing Errors ◽  
Vancomycin Resistant ◽  
Random Sequencing

Abstract Background Recognition of nosocomial outbreaks with antimicrobial resistant (AMR) pathogens and appropriate infection prevention measures are essential to limit the consequences of AMR pathogens to patients in hospitals. Because unrelated, but genetically similar AMR pathogens may circulate simultaneously, rapid high-resolution molecular typing methods are needed for outbreak management. We compared amplified fragment length polymorphism (AFLP) and whole genome sequencing (WGS) during a nosocomial outbreak of vancomycin-resistant Enterococcus faecium (VRE) that spanned 5 months. Methods Hierarchical clustering of AFLP profiles was performed using unweighted pair-grouping and similarity coefficients were calculated with Pearson correlation. For WGS-analysis, core single nucleotide polymorphisms (SNPs) were used to calculate the pairwise distance between isolates, construct a maximum likelihood phylogeny and establish a cut-off for relatedness of epidemiologically linked VRE isolates. SNP-variations in the vanB gene cluster were compared to increase the comparative resolution. Technical replicates of 2 isolates were sequenced to determine the number of core-SNPs derived from random sequencing errors. Results Of the 721 patients screened for VRE carriage, AFLP assigned isolates of 22 patients to the outbreak cluster. According to WGS, all 22 isolates belonged to ST117 but only 21 grouped in a tight phylogenetic cluster and carried vanB resistance gene clusters. Sequencing of technical replicates showed that 4–5 core-SNPs were derived by random sequencing errors. The cut-off for relatedness of epidemiologically linked VRE isolates was established at ≤7 core-SNPs. The discrepant isolate was separated from the index isolate by 61 core-SNPs and the vanB gene cluster was absent. In AFLP analysis this discrepant isolate was indistinguishable from the other outbreak isolates, forming a cluster with 92% similarity (cut-off for identical isolates ≥90%). The inclusion of the discrepant isolate in the outbreak resulted in the screening of 250 patients and quarantining of an entire ward. Conclusion AFLP was a rapid and affordable screening tool for characterising hospital VRE outbreaks. For in-depth understanding of the outbreak WGS was needed. Compared to AFLP, WGS provided higher resolution typing of VRE isolates with implications for outbreak management.

scholarly journals Nosocomial Outbreak of Drug-Resistant Streptococcus pneumoniae Serotype 9V in an Adult Respiratory Medicine Ward

2016 ◽  
Vol 55 (3) ◽  
pp. 776-782 ◽  
Author(s):  
Elita Jauneikaite ◽  
Zareena Khan-Orakzai ◽  
Georgia Kapatai ◽  
Susannah Bloch ◽  
Julie Singleton ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Lower Respiratory Tract ◽  
Sequence Type ◽  
Resistance Pattern ◽  
Whole Genome ◽  
Drug Resistant ◽  
Nosocomial Outbreak ◽  
Content Type ◽  
Rapid Transmission ◽  
Case Presentations

ABSTRACT Streptococcus pneumoniae infections arising in hospitalized patients are often assumed to be sporadic and linked to community acquisition. Here, whole-genome sequencing was used to demonstrate nosocomial acquisition of antimicrobial-resistant sequence type 156 (ST156) serotype 9V S. pneumoniae in 3 respiratory patients that resulted in two bacteremias and one lower respiratory tract infection. Two of the cases arose in patients who had recently been discharged from the hospital and were readmitted from the community. Nosocomial spread was suspected solely because of the highly unusual resistance pattern and case presentations within 24 h of one another. The outbreak highlights the potential for rapid transmission and the short incubation period in the respiratory ward setting.

Nosocomial Outbreak of Systemic Candidosis Associated with Parenteral Nutrition

1990 ◽  
Vol 11 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Maria Luisa Moro ◽  
Claudio Maffei ◽  
Esther Manso ◽  
Giulia Morace ◽  
Luciano Polonelli ◽  
...  
Keyword(s):  
Parenteral Nutrition ◽  
Nosocomial Outbreak

scholarly journals Nosocomial outbreak of KPC-2- and NDM-1-producing Klebsiella pneumoniae in a neonatal ward: a retrospective study

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Jing Yu ◽  
Kun Tan ◽  
Zhihui Rong ◽  
Yue Wang ◽  
Zhongju Chen ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Outbreak ◽  
Neonatal Ward

scholarly journals Multicluster nosocomial outbreak of parainfluenza virus type 3 infection in a pediatric oncohematology unit: a phylogenetic study

Haematologica ◽  
2009 ◽  
Vol 94 (6) ◽  
pp. 833-839 ◽  
Author(s):  
A. Piralla ◽  
E. Percivalle ◽  
A. Di Cesare-Merlone ◽  
F. Locatelli ◽  
G. Gerna
Keyword(s):  
Virus Type ◽  
Parainfluenza Virus ◽  
Phylogenetic Study ◽  
Nosocomial Outbreak ◽  
Type 3

Development of a nosocomial outbreak investigation database

2005 ◽  
Vol 59 (3) ◽  
pp. 215-219 ◽  
Author(s):  
K. Makimoto ◽  
N. Ashida ◽  
N. Qureshi ◽  
T. Tsuchida ◽  
A. Sekikawa
Keyword(s):  
Outbreak Investigation ◽  
Nosocomial Outbreak

Nosocomial outbreak of Klebsiella pneumoniae harbouring blaKPC-3 in France subsequent to a patient transfer from Italy

2012 ◽  
Vol 39 (5) ◽  
pp. 448-449 ◽  
Author(s):  
Gaelle Cuzon ◽  
Thierry Naas ◽  
Marie-Claude Demachy ◽  
Patrice Nordmann
Keyword(s):  
Klebsiella Pneumoniae ◽  
Patient Transfer ◽  
Nosocomial Outbreak
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