Cold treatment promotes in vitro germination of two endangered Lachenalia species

2021 ◽  
Vol 142 ◽  
pp. 430-432
Author(s):  
Małgorzata Maślanka ◽  
Anna Kapczyńska
Keyword(s):  
Cold Treatment ◽  
In Vitro Germination

In vitro germination of three Cypripedium species in relation to time of seed collection, media, and cold treatment

1993 ◽  
Vol 71 (6) ◽  
pp. 879-885 ◽  
Author(s):  
M. A. De Pauw ◽  
W. R. Remphrey
Keyword(s):  
Seed Germination ◽  
Cold Treatment ◽  
Subsequent Development ◽  
The Other ◽  
In Vitro Germination ◽  
Optimum Time ◽  
Seed Collection ◽  
Seed Maturity ◽  
Better Than

To maximize the in vitro seed germination of three Cypripedium species, C. candidum Muhl. ex Willd., C. reginae Walt., and C. calceolus var. parviflorum Salisb. Fern., the optimum time of seed collection and the suitability of various media were studied. A cold treatment was investigated for C. candidum. Seed pods were collected at regular intervals after pollination, ranging from green pods at 5 weeks to dry undehisced pods at 12 weeks. Seed was inoculated onto three media, Harvais, Van Waes and Debergh, and a modified Norstog and germination was assessed at 4-week intervals for 20 weeks. Cypripedium reginae germinated better than the other two species. Except for C. candidum in 1989 when germination peaked for seed collected at 6 weeks after pollination, seed collected at 8 weeks after pollination had the highest germination. After 8 weeks, germination decreased sharply in all species, dropping below 1% for C. candidum. There were differences among media but the effects on germination were not consistent and depended on the year, the date of collection, and the species. Subsequent development of protocorms was superior in all cases on the modified Norstog medium. The effect of cold treatment on germination of C. candidum was variable and therefore its importance is questionable. Key words: in vitro, germination, Cypripedium, lady's slippers, seed maturity.

scholarly journals In Vitro Germination and Dormancy Responses of Hydrangea macrophylla and Hydrangea paniculata Seeds to Ethyl Methane Sulfonate and Cold Treatment

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 764-769 ◽  
Author(s):  
Stephen Patrick Greer ◽  
Timothy A. Rinehart
Keyword(s):  
Cold Treatment ◽  
In Vitro Germination ◽  
Ethyl Methane Sulfonate ◽  
Optimal Conditions ◽  
Methane Sulfonate ◽  
Untreated Seed ◽  
Ethyl Methane ◽  
Hydrangea Macrophylla ◽  
Treated Seed

To determine the optimal conditions for mutagenesis of Hydrangea macrophylla (Thunb.) Ser. and Hydrangea paniculata Siebold, cold-treated and untreated seed from representative cultivars were exposed to 0.5%, 2.5%, and 5.0% ethyl methane sulfonate (EMS). Most untreated H. macrophylla seed exposed to 2.5% and 5.0% EMS had substantially lower germination percentages. H. paniculata seed germination percentages did not differ from controls until EMS concentrations reached 5.0%. Cold treatment of H. macrophylla and H. paniculata seed made germination more tolerant to all concentrations of EMS tested and increased germination of most H. macrophylla cultivars when compared with lower dosages. Germination of cold-treated seed from H. paniculata cultivars rose substantially above control levels even at the highest dosages. For the most part, we observed more nondormant seed, or seed available for germination, but less viability with increasing EMS dosage in untreated Hydrangea L. seed versus controls. In contrast, cold-treated seed displayed higher levels of mutagen tolerance and nondormancy versus controls when exposed to these same doses of EMS.

In vitro germination protocol for the propagation of the endangered butternut tree (Juglans cinerea L.)

2019 ◽  
Vol 20 (2) ◽  
pp. 117-122 ◽  
Author(s):  
Martin Williams ◽  
Kathleen Forbes ◽  
Charlene Williams ◽  
Tannis Beardmore
Keyword(s):  
In Vitro Germination ◽  
Juglans Cinerea

scholarly journals Low temperature storage and in vitro germination of cherimoya (Annona cherimola Mill.) pollen

2006 ◽  
Vol 108 (1) ◽  
pp. 91-94 ◽  
Author(s):  
J. Lora ◽  
M.A. Pérez de Oteyza ◽  
P. Fuentetaja ◽  
J.I. Hormaza
Keyword(s):  
Low Temperature ◽  
In Vitro Germination ◽  
Low Temperature Storage ◽  
Annona Cherimola ◽  
Temperature Storage

In vitro germination and transient GFP expression of American chestnut (Castanea dentata) pollen

2005 ◽  
Vol 25 (5) ◽  
pp. 450-456 ◽  
Author(s):  
Danilo D. Fernando ◽  
Javonna L. Richards ◽  
Julie R. Kikkert
Keyword(s):  
Castanea Dentata ◽  
American Chestnut ◽  
In Vitro Germination ◽  
Gfp Expression

scholarly journals Super Cooling Point Phenotypes and Cold Resistance in Hyles euphorbiae Hawk Moths from Different Climate Zones

Diversity ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 207
Author(s):  
Hana Daneck ◽  
Matthias Benjamin Barth ◽  
Martin Geck ◽  
Anna K. Hundsdoerfer
Keyword(s):  
Environmental Conditions ◽  
Cold Hardiness ◽  
Cold Treatment ◽  
Underlying Mechanism ◽  
Frost Tolerance ◽  
Cold Sensitivity ◽  
Cold Hardy ◽  
Hyles Euphorbiae

The spurge hawkmoth Hyles euphorbiae L. (Sphingidae) comprises a remarkable species complex with still not fully resolved taxonomy. Its extensive natural distribution range covers diverse climatic zones. This predestinates particular populations to cope with different local seasonally unfavorable environmental conditions. The ability of the pupae to overcome outer frosty conditions is well known. However, the differences between two main ecotypes (‘euphorbiae’ and ‘tithymali’) in terms of the inherent degree of frost tolerance, its corresponding survival strategy, and underlying mechanism have not been studied in detail so far. The main aim of our study was to test the phenotypic exhibition of pupae (as the relevant life cycle stadia to outlast unfavorable conditions) in response to combined effects of exogenous stimuli, such as daylight length and cooling regime. Namely, we tested the turnout of subitan (with fast development, unadapted to unfavorable conditions) or diapause (paused development, adapted to unfavorable external influences and increased resistance) pupae under different conditions, as well as their mortality, and we measured the super cooling point (SCP) of whole pupae (in vivo) and pupal hemolymph (in vitro) as phenotypic indicators of cold acclimation. Our results show higher cold sensitivity in ‘tithymali’ populations, exhibiting rather opportunistic and short-termed cold hardiness, while ‘euphorbiae’ produces a phenotype of seasonal cold-hardy diapause pupae under a combined effect of short daylight length and continuous cold treatment. Further differences include the variability in duration and mortality of diapause pupae. This suggests different pre-adaptations to seasonal environmental conditions in each ecotype and may indicate a state of incipient speciation within the H. euphorbiae complex.

Control of browning during seed germination of Meizotropis pellita: a rare and an endangered plant

2021 ◽  
Vol 16 (8) ◽  
pp. 118-120
Author(s):  
Renu Chandola ◽  
Anshulika Upadhyaya
Keyword(s):  
Seed Germination ◽  
Culture Media ◽  
Light Condition ◽  
Endangered Plant ◽  
In Vitro Germination ◽  
Light Conditions ◽  
Low Intensity ◽  
Browning Reaction ◽  
Threatened Plant Species

Meizotropis pellita is a rare, endangered and threatened plant species found in Patwadanger forest. In nature this species grows from rootstock in precise and sensitive habitats. However, phenolic compounds provoke browning reaction which is responsible for lethal browning in plant tissue culture and hindering in vitro germination. The aim of this study was to minimize the effect of phenolic compound during seed germination. We evaluate different antibrowning treatments in Meizotropis pellita seeds. The seeds were pretreated in Polyvinylpyrrolidone and then treated seeds were cultured on MS media and sterilized seeds were directly inoculated on PVP containing MS media. These culture media incubate under dark and light conditions at different durations respectively. Our results showed that addition of antibrowning agent, PVP inhibit onset of browning. Dark and light condition also played a crucial role on onset of browning. Delayed and low intensity of browning were observed in case of seeds that were inoculated on MS media containing PVP and were kept in dark. The present research offers a positive in vitro seed germination protocol for Meizotropis pellita conservation.

Sign in / Sign up

Export Citation Format

Share Document