Individual and combined applications of biochar and pyroligneous acid mitigate dissemination of antibiotic resistance genes in agricultural soil

2021 ◽  
pp. 148962
Author(s):  
Hao Zheng ◽  
Nianlin Feng ◽  
Tianning Yang ◽  
Mei Shi ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Agricultural Soil ◽  
Antibiotic Resistance Genes ◽  
Pyroligneous Acid

scholarly journals Abundance of Antibiotic Resistance Genes in Bacteriophage following Soil Fertilization with Dairy Manure or Municipal Biosolids, and Evidence for Potential Transduction

2015 ◽  
Vol 81 (22) ◽  
pp. 7905-7913 ◽  
Author(s):  
Joseph Ross ◽  
Edward Topp
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Horizontal Transfer ◽  
Crop Production ◽  
Agricultural Soil ◽  
Antibiotic Resistance Genes ◽  
Dairy Manure ◽  
Soil Microbiome ◽  
Resistant Bacteria ◽  
Municipal Biosolids

ABSTRACTAnimal manures and municipal biosolids recycled onto crop production land carry antibiotic-resistant bacteria that can influence the antibiotic resistome of agricultural soils, but little is known about the contribution of bacteriophage to the dissemination of antibiotic resistance genes (ARGs) in this context. In this work, we quantified a set of ARGs in the bacterial and bacteriophage fractions of agricultural soil by quantitative PCR. All tested ARGs were present in both the bacterial and phage fractions. We demonstrate that fertilization of soil with dairy manure or human biosolids increases ARG abundance in the bacterial fraction but not the bacteriophage fraction and further show that pretreatment of dairy manure can impact ARG abundance in the bacterial fraction. Finally, we show that purified bacteriophage can confer increased antibiotic resistance to soil bacteria when combined with selective pressure. The results indicate that soilborne bacteriophage represents a substantial reservoir of antibiotic resistance and that bacteriophage could play a significant role in the horizontal transfer of resistance genes in the context of an agricultural soil microbiome. Overall, our work reinforces the advisability of composting or digesting fecal material prior to field application and suggests that application of some antibiotics at subclinical concentrations can promote bacteriophage-mediated horizontal transfer of ARGs in agricultural soil microbiomes.

Dissipation of antibiotic resistance genes in manure-amended agricultural soil

2021 ◽  
Vol 787 ◽  
pp. 147582
Author(s):  
Liang-Ying He ◽  
Lun-Kai He ◽  
Fang-Zhou Gao ◽  
Dai-Ling Wu ◽  
Hai-Yan Zou ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Agricultural Soil ◽  
Antibiotic Resistance Genes

scholarly journals Pyroligneous acid mitigated dissemination of antibiotic resistance genes in soil

2020 ◽  
Vol 145 ◽  
pp. 106158
Author(s):  
Hao Zheng ◽  
Ruirui Wang ◽  
Qian Zhang ◽  
Jian Zhao ◽  
Fengmin Li ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Pyroligneous Acid

Phage particles harboring antibiotic resistance genes in fresh-cut vegetables and agricultural soil

2018 ◽  
Vol 115 ◽  
pp. 133-141 ◽  
Author(s):  
Olatz Larrañaga ◽  
Maryury Brown-Jaque ◽  
Pablo Quirós ◽  
Clara Gómez-Gómez ◽  
Anicet R. Blanch ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Agricultural Soil ◽  
Antibiotic Resistance Genes ◽  
Fresh Cut
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