Optimization of thaumatin extraction by aqueous two-phase system (ATPS) using response surface methodology (RSM)

2008 ◽  
Vol 62 (3) ◽  
pp. 702-708 ◽  
Author(s):  
A.L. Ahmad ◽  
C.J.C. Derek ◽  
M.M.D. Zulkali
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Optimization of Aqueous Two-Phase System (ATPS) of Recombinant Bromelain by Response Surface Methodology

2018 ◽  
Vol 7 (4.30) ◽  
pp. 377
Author(s):  
Zatul Iffah Mohd Arshad ◽  
Azura Amid
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Partition Coefficient ◽  
Response Surface ◽  
Potassium Phosphate ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Crude Sample ◽  
Face Centered

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.

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