Comparative evaluation of a new commercial recombinant ELISA and the complement fixation test for the diagnosis of Chlamydophila abortus infection in naturally infected flocks in Tunisia

2006 ◽  
Vol 66 (1-3) ◽  
pp. 58-63 ◽  
Author(s):  
A. Rekiki ◽  
S. Hammami ◽  
A. Rodolakis
Keyword(s):  
Comparative Evaluation ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Chlamydophila Abortus

scholarly journals Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams

2012 ◽  
Vol 47 (No. 7) ◽  
pp. 195-198 ◽  
Author(s):  
M. Trávníček ◽  
D. Kováčová ◽  
Bhide MR ◽  
P. Zubrický ◽  
L. Čisláková
Keyword(s):  
Complement Fixation Test ◽  
Indirect Elisa ◽  
Complement Fixation ◽  
Field Evaluation ◽  
Fixation Test ◽  
Igg Antibodies ◽  
Herd Level ◽  
Chlamydophila Abortus ◽  
Moderate Agreement ◽  
Sera Samples

Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%, whereas, in case of rams seroprevalence was 0% by using complement fixation test. Using iELISA in the same groups the seroprevalences observed were, 24.24% in goats, 11.30% in sheep and 5.30% in rams. Indirect ELISA was found to be comparatively more sensitive than CF test in all three groups of animals for detection of IgG antibodies against Chlamydophila abortus. The iELISA used in this study can be used for screening at herd level like CF test, as there is moderate agreement (Kappa – 0.426) between these two tests.

Comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders

2011 ◽  
Vol 169 (19) ◽  
pp. 495-495 ◽  
Author(s):  
I. Khan ◽  
L. H. Wieler ◽  
F. Melzer ◽  
M. Gwida ◽  
V. L. d. A. Santana ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Assessment of a complement fixation test to detect Mycoplasma hyopneumoniae infection in pigs

1984 ◽  
Vol 61 (7) ◽  
pp. 216-218
Author(s):  
L. C. LLOYD ◽  
R. T. BADMAN ◽  
J. R. ETHERIDGE ◽  
K. McKECHNIE ◽  
H. IYER
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycoplasma Hyopneumoniae ◽  
Fixation Test
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