Detection of Toxoplasma gondii and Sarcocystis tenella in indigenous Cornigliese sheep in Italy using serological and molecular methods

2016 ◽  
Vol 135 ◽  
pp. 13-16 ◽  
Author(s):  
C. Bacci ◽  
A. Vismarra ◽  
B. Passeri ◽  
F. Sciarrone ◽  
C. Mangia ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Methods ◽  
Sarcocystis Tenella

Microbiological quality of blue mussels (Mytilus edulis) in Nunavik, Quebec: a pilot study

2010 ◽  
Vol 56 (11) ◽  
pp. 968-977 ◽  
Author(s):  
Benoît Lévesque ◽  
Christine Barthe ◽  
Brent R. Dixon ◽  
Lorna J. Parrington ◽  
Daniel Martin ◽  
...  
Keyword(s):  
Pilot Study ◽  
Toxoplasma Gondii ◽  
Mytilus Edulis ◽  
Clostridium Perfringens ◽  
Microbiological Quality ◽  
Molecular Methods ◽  
Fecal Indicators ◽  
Salmonella Spp ◽  
Blue Mussels ◽  
Cryptosporidium Spp

This pilot study was aimed at documenting the presence of fecal indicators and enteric pathogens in blue mussels ( Mytilus edulis ) from 6 communities in Nunavik, Quebec. One to four 2 kg samples of mussels were collected at low tide in each community. Samples were investigated by enumeration methods for the fecal indicators enterococci, Escherichia coli , F-specific coliphages, Clostridium perfringens , and by molecular identification for the pathogens norovirus, Salmonella spp., Campylobacter jejuni , Campylobacter coli , and Campylobacter lari , verocytotoxin-producing E. coli (particularly serovar O157:H7), Shigella spp., and Yersinia enterocolitica . In 5 communities, the presence of Giardia duodenalis and Cryptosporidium spp. was also tested by microscopy and molecular methods and that of Toxoplasma gondii was tested by molecular methods. Apart from small quantities of Clostridium perfringens in 2 samples, no bacterial or viral pathogens were detected in the mussels. Toxoplasma gondii was also not detected. However, G. duodenalis and Cryptosporidium spp. were present in 18% and 73% of the samples investigated for these pathogens, respectively. When considering the indicators and the viral and bacterial pathogens investigated, the mussels examined were of good microbiological quality, but considering the presence of potentially zoonotic protozoa, it should be recommended that consumers cook the molluscs well before eating them.

scholarly journals Performance of three molecular methods for detection of Toxoplasma gondii in pork

2019 ◽  
Vol 14 ◽  
pp. e00038
Author(s):  
Nadja S. Bier ◽  
Gereon Schares ◽  
Annette Johne ◽  
Annett Martin ◽  
Karsten Nöckler ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Methods

Detection of Toxoplasma gondii oocysts in environmental soil samples using molecular methods

2008 ◽  
Vol 28 (6) ◽  
pp. 599-605 ◽  
Author(s):  
A. Lass ◽  
H. Pietkiewicz ◽  
E. Modzelewska ◽  
A. Dumètre ◽  
B. Szostakowska ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Soil Samples ◽  
Molecular Methods

scholarly journals Prevalence of Toxoplasma gondii Infection in Healthy Volunteer Blood Donors Using Serological and Molecular Methods from Chaharmahal and Bakhtiari Province, Southwest Iran

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Kourosh Manouchehri Naeini ◽  
Ehsan Heidari Soureshjani ◽  
Mahnaz Jafari ◽  
Shahrbanoo Parchami ◽  
Gharib Karimi ◽  
...  
Keyword(s):  
Healthy Volunteer ◽  
Toxoplasma Gondii ◽  
Blood Donors ◽  
Molecular Methods ◽  
Toxoplasma Gondii Infection ◽  
Southwest Iran

scholarly journals Copro-PCR in the detection and confirmation of Toxoplasma gondii oocysts in feces of stray and domiciled cats

2021 ◽  
Vol 30 (2) ◽  
Author(s):  
Jaqueline Ataíde Silva Lima da Igreja ◽  
Hanstter Hallison Alves Rezende ◽  
Jade de Oliveira Melo ◽  
João Luís Garcia ◽  
Felippe Danyel Cardoso Martins ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Genetic Material ◽  
High Sensitivity ◽  
Molecular Techniques ◽  
Molecular Methods ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Fecal Tests

Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction in 149 fecal samples from stray and domiciled cats, using three distinct markers (B5-B6, 18S and 529bp RE). Oocysts of T. gondii/H. hammondi were detected in 15.4% by parasitology fecal tests (PFT), and 4% of these oocysts were positively identified as T. gondii by Copro-PCR. The presence of T. gondii genetic material was detected in 16.1%, but 12% of the samples that tested positive by Copro-PCR were negative in PFT. Samples with discordant results were subjected to a new Copro-PCR with 18S marker and a 529, and of the 17 samples, 9 contained T. gondii genetic material. A comparison of the PFT and the molecular methods showed the latter was more sensitive, since it detected 22.1% while the PFT detected 15.4%. Demonstrating the high sensitivity and specificity of the Copro-PCR, particularly with the association of primers (k=0.809), but also confirms the importance of using molecular techniques in laboratories, since Copro-PCR was able to detect samples considered negative by PFT.

scholarly journals Aspectos ultraestruturais do processo de divisão do Toxoplasma gondii

1974 ◽  
Vol 8 (1) ◽  
pp. 45-65 ◽  
Author(s):  
Wanderley de Souza
Keyword(s):  
Toxoplasma Gondii ◽  
Eimeria Bovis ◽  
Sarcocystis Tenella

Neste trabalho é feita uma revisão sobre alguns aspectos biológicos do Toxoplasma gondii, principalmente sobre a ultraestrutura da forma interfásica e as modificações ultraestruturais que ocorrem no parasito durante o seu processo de divisão. Considera-se inicialmente o processo de divisão binária admitindo-se, porém, a possibilidade de que as imagens interpretadas como senão de divisão binária representem estágios da divisão por endodiogenia. Quanto à endodiogenia descrevem-se as alterações que ocorrem na "parasito mãe" durante o processo de formação dos dois "parasitos filhos". Este processo é semelhante no Toxoplasma gondii, Besnoitia jellisoni, Sarcocystis tenella e Frenkelia. Discute-se a possibilidade da formação de mais de dois "parasitos filhos" por um processo de endopoligenia, bem como o processo de esquizogonia. Os resultados mais recentes mostram que não existe esquizogonia nas formas vsgetativas do Toxoplasma gondii, senão que as imagens interpretadas como tal, ao microscópio ótico, são o resultado de endodiogenias sucessivas em que os endozoitas formados permanecem ligados entre si pela região posterior. A esquizogonia é, no entanto, encontrada nas formas que se desenvolvem no interior de células epiteliais do intestino do gato, que é o hospedeiro definitivo do Toxoplasma gondii. Discute-se o conceito de esquizogonia, comparando-o em três protozoários: Eimeria bovis, E. callospermophili e Plasmodium juxtanucleare, que apresentam diferenças entre si quanto ao processo de iniciação da individualização dos "parasitos filhos". Refere-se à recente hipótese que considera a endodiogenia como o processo fundamental de divisão dos esporozoárlos, ocorrendo na fase final da esquizogonia. Finalmente é acentuado o papel que a microscopia eletrônica aliada às modernas técnicas de citoquímica e imunocitoquimica poderá desempenhar no sentido de um melhor conhecimento da biologia do Toxoplasma gondii e da fisiopatogenia da Toxoplasmose.

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