Phylogenetic status and diet of red fox (Vulpes vulpes griffithii) inhabiting Ayubia National Park, Pakistan

The red fox (Vulpes vulpes) is a medium-sized carnivore that occurs in different regions of Pakistan, however, still lacks scientific data on its ecology and distribution. The current study investigated the phylogenetic status and diet of the red fox (V.v. griffithii) occurring in Ayubia National Park, Pakistan. Through camera trapping and molecular analysis, we confirmed the occurrence of red fox in the study area. Based on mitochondrial cytochrome B (304 bp) and limited sampling, nearly all red foxes of Ayubia National Park and surrounding Himalayan ranges fall within Holarctic maternal lineage, whereas red foxes found in plains of Pakistan are part of the basal Palearctic maternal lineage. Using 32 scats, we found that red fox diet comprises of 80% animal-based prey species (both wild and domestic) and 19% plant matter. The wild animal prey species included Cape hare (Lepus capensis) and flying squirrel (Pteromyini sp.), which constituted 17% and 15% of diet, respectively. Red foxes infrequently consumed House mouse (Mus musculus), Himalayan Palm civet (Paguma larvata) and sheep (Ovis aries), each comprising around 6% to 9% of red fox diet. The fox species also scavenged on domestic donkey opportunistically. Based on our sampling, our study suggests that the red fox (V.v. griffithii) that occurs in Ayubia National Park and across the lesser Himalayan ranges belongs to Holarctic maternal lineage. The study also highlights consumption of plant seeds by red foxes, indicating it may play an important ecological role in seed dispersal in Ayubia National Park.

Complete Genomic Sequencing of Canine Distemper Virus With Nanopore Technology During an Epizootic Event

Canine distemper virus (CDV) endangers a wide range of wild animal populations and can cross species barriers, representing a significant conservational and animal health risk around the globe. During spring to autumn 2021, according to our current estimates a minimum of 50 wild live red foxes (Vulpes vulpes) died of CDV in Hungary, with CDV lesions. Oral, nasal and rectal swab samples were RT-PCR screened for Canine Distemper Virus from red fox carcasses. To investigate in more detail the origins of these CDV strains, 19 complete genomes were sequenced with a pan-genotype CDV-specific amplicon-based sequencing method developed by our laboratory and optimized for Oxford Nanopore Technologies platform. Phylogenetic analysis of the complete genomic sequences and separately the hemagglutinin gene sequences revealed the role of the Europe lineage of CDV as a causative agent for the current epizootic. Here we highlight the growing importance of fast developing rapid sequencing technologies to aid rapid response activities during epidemics or epizootic events. We also emphasize the urgent need for improved surveillance of CDV, considering the epizootic capability of enzootic strains as reported in the current study. For such future efforts, we provide a novel NGS protocol, which facilitates future genomic surveillance studies.

Rabies virus spread in central and north-eastern regions of Ukraine

Annually, 30,000-45,000 cases of rabies in animals and humans are reported worldwide. The aim of the study was to present data on the incidence of rabies among humans and animals in central and north-eastern regions of Ukraine (Poltava and Sumy) between the years 2007 and 2020. The statistical data were obtained from the anti-epizootic measures division at the Department of Food Safety and Veterinary Medicine of the State Food and Consumer Service in the Poltava and Sumy regions of Ukraine. Between 2012 and 2020, the largest number of bite cases in the Poltava region were recorded in 2012 (4,234 cases), and the lowest in 2018 (1,951 cases). The vast majority of bites were caused by dogs and cats that had an owner. During the successive years, the number of rabies cases increased. Between 2017 and 2019, over 50% of rabies cases in the Sumy region occurred in domestic animals. Although combating rabies among dogs through vaccination, as well as extermination of homeless animals, may reduce the incidence of rabies in humans, rabies in dogs is still not uncommon in the Poltava and Sumy regions of Ukraine. Moreover, rabid dogs in these two regions still account for over 90% of rabies cases and 99% of deaths due to rabies among humans. The red fox was the leader in the incidence of rabies among wild animals between 2009 and 2019. In the Poltava region, the highest number of rabies cases occurred in 2017 (10 cases), whereas in the Sumy region the peak if infections was observed in 2010 (42 cases). Rabies in animals tended to decrease due to the oral immunization of free-ranging animals. It is difficult, however, to assess the overall global situation of rabies using data solely on human mortality. Rabies is not a purely human disease. It is rather a disease that occurs in wild and domestic animals, from which it may be transferred to humans. Therefore, a more accurate prediction of its impact should include an assessment of its prevalence in animal populations, especially pets, and the costs associated with preventing the spread of rabies from animals to humans. Effective measures for prevention and eradication of rabies, as well as better knowledge, are crucial to check further spread of the disease. Obligatory preventive measures should include vaccination of pets and oral immunization of red foxes.

A Unique Case of Fatal Coinfection Caused by Leptospira spp. and Hepatozoon canis in a Red Fox Cub (Vulpes vulpes)

Red foxes are the most abundant wild carnivore species in Europe commonly exposed to pathogenic Leptospira and Hepatozoon canis. Despite high seroprevalence, the clinical disease caused by these pathogens in red foxes has never been reported. Herein, we report the first-ever case of a fatal Leptospira spp. and H. canis coinfection in a two-month-old red fox cub with acute haemolytic anaemia, mild bronchopneumonia, intraalveolar haemorrhage, and tubulonephrosis. The presence of pathogenic Leptospira spp. DNA was detected in the kidney and lung tissues of the infected animal. In contrast to our previous knowledge, we believe that such fatal cases due to concomitant infection by Leptospira spp. and H. canis, especially in young animals, may commonly occur in nature. However, further studies are required to identify other factors that possibly contribute to the severity and the pathogenic effect of Leptospira spp. and H. canis infections in red foxes.

Citizen Science and Its Contribution to the Conservation of Sacramento Valley Red Foxes in California

<p>Red foxes found in the Sacramento Valley of California, referred to as SV red foxes throughout this thesis, were assumed to be of non-native ancestry until recent genetic testing confirmed their historical link to the area. The development of an effective conservation strategy for SV red foxes is currently underway, but was initially hindered by the lack of details regarding habitat requirements, occupancy patterns, and population sizes. In 2007, the University of California, Davis launched a fox reporting website for the public to document any fox sightings. Researchers actively pursued the public fox sighting reports from 2007-2009 and found 51 active red fox dens, 48 of which were native SV red fox dens, throughout the Sacramento Valley based on the locations provided in the reports. The objectives of my research are to answer the following questions: (1) What can the data provided in the previously obtained fox sighting reports reveal about the website trends and fox ecology? (2) What types of relationships occur between the public and the SV red fox research efforts, and how can the participants’ experience be improved to ensure long-term participation? (3) Can a recently developed presence-only SV habitat model be used as a filter for future incoming reports, and what are the relationships between the reports with a high probability of fox presence and its associate report components? In chapter one, I assess how effective the public sighting reports (n= 248) were in: assisting researchers with locating SV red fox dens, providing information about habitat preferences of the SV red foxes, and supplying insights of the website. The results showed that the distance from Yolo County, where the highest amount of SV red fox dens is located and where the initial study was conducted, can explain 38% of the variation in the quantity of reports collected from the website. In chapter two, I administered an online survey to the residents of the Sacramento Valley to gauge their outlook on SV red foxes and their opinions of the fox reporting website. The survey respondents included both previous website visitors and new website visitors. I summarized the responses (n= 310 total, n= 210 complete) and ran a series of statistical analyses to evaluate the social characteristics of the participants utilizing the website. Overall, the majority of participants responded positively to the website, declaring it interesting and helpful. However, there is a low rate of return visitors. The main users of the fox sighting website are older participants and/or participants with a higher level of education. In the third chapter of this research, I overlaid the fox sighting report locations onto the SV habitat model using ERSI® ArcMap v 10.0 and coded them for habitat suitability values. Analysing previously obtained fox sighting reports provided the necessary insight to define the criteria for the data filter. By implementing a habitat model filter at moderate habitat suitability levels, researchers can reduce the time it takes them to validate the public data received. However, a coinciding filter recording distribution frequency for report locations should also be implemented to compensate for dens located in low habitat suitability levels. Prior to this research, the data collected from the public had never been evaluated for purposes other than locating potential SV red foxes. By reviewing feedback from the participants, I was able to characterise the participants using the fox reporting website and assess their general SV red fox knowledge. The findings of my research also lead to insights about SV red fox ecology. However, the major finding in this research shows that the majority of local citizens participating in this study indicated awareness of SV red foxes nativity and endangerment. The methods used in my research can be applied to other citizen science projects, to improve the overall efficiency and effectiveness of collecting data.</p>

Citizen Science and Its Contribution to the Conservation of Sacramento Valley Red Foxes in California

<p>Red foxes found in the Sacramento Valley of California, referred to as SV red foxes throughout this thesis, were assumed to be of non-native ancestry until recent genetic testing confirmed their historical link to the area. The development of an effective conservation strategy for SV red foxes is currently underway, but was initially hindered by the lack of details regarding habitat requirements, occupancy patterns, and population sizes. In 2007, the University of California, Davis launched a fox reporting website for the public to document any fox sightings. Researchers actively pursued the public fox sighting reports from 2007-2009 and found 51 active red fox dens, 48 of which were native SV red fox dens, throughout the Sacramento Valley based on the locations provided in the reports. The objectives of my research are to answer the following questions: (1) What can the data provided in the previously obtained fox sighting reports reveal about the website trends and fox ecology? (2) What types of relationships occur between the public and the SV red fox research efforts, and how can the participants’ experience be improved to ensure long-term participation? (3) Can a recently developed presence-only SV habitat model be used as a filter for future incoming reports, and what are the relationships between the reports with a high probability of fox presence and its associate report components? In chapter one, I assess how effective the public sighting reports (n= 248) were in: assisting researchers with locating SV red fox dens, providing information about habitat preferences of the SV red foxes, and supplying insights of the website. The results showed that the distance from Yolo County, where the highest amount of SV red fox dens is located and where the initial study was conducted, can explain 38% of the variation in the quantity of reports collected from the website. In chapter two, I administered an online survey to the residents of the Sacramento Valley to gauge their outlook on SV red foxes and their opinions of the fox reporting website. The survey respondents included both previous website visitors and new website visitors. I summarized the responses (n= 310 total, n= 210 complete) and ran a series of statistical analyses to evaluate the social characteristics of the participants utilizing the website. Overall, the majority of participants responded positively to the website, declaring it interesting and helpful. However, there is a low rate of return visitors. The main users of the fox sighting website are older participants and/or participants with a higher level of education. In the third chapter of this research, I overlaid the fox sighting report locations onto the SV habitat model using ERSI® ArcMap v 10.0 and coded them for habitat suitability values. Analysing previously obtained fox sighting reports provided the necessary insight to define the criteria for the data filter. By implementing a habitat model filter at moderate habitat suitability levels, researchers can reduce the time it takes them to validate the public data received. However, a coinciding filter recording distribution frequency for report locations should also be implemented to compensate for dens located in low habitat suitability levels. Prior to this research, the data collected from the public had never been evaluated for purposes other than locating potential SV red foxes. By reviewing feedback from the participants, I was able to characterise the participants using the fox reporting website and assess their general SV red fox knowledge. The findings of my research also lead to insights about SV red fox ecology. However, the major finding in this research shows that the majority of local citizens participating in this study indicated awareness of SV red foxes nativity and endangerment. The methods used in my research can be applied to other citizen science projects, to improve the overall efficiency and effectiveness of collecting data.</p>

Fox (Vulpes vulpes) involvement identified in a series of cat carcass mutilations

This study was designed to identify the cause of mutilation and death in 32 cats, part of a larger cohort found dead in Greater London, the United Kingdom, between 2016 and 2018. At the time, discussion in the media led to concerns of a human serial cat killer (dubbed The Croydon Cat Killer) pursuing domestic cats, causing a state of disquietude. Given the link between animal abuse and domestic violence, human intervention had to be ruled out. Using a combination of DNA testing, computed tomography imaging, and postmortem examination, no evidence was found to support any human involvement. Instead, a significant association between cat carcass mutilation and the presence of fox DNA was demonstrated. Gross examination identified shared characteristics including the pattern of mutilation, level of limb or vertebral disarticulation, wet fur, wound edges with shortened fur, and smooth or irregular contours, and marks in the skin, muscle, and bone consistent with damage from carnivore teeth. Together these findings supported the theory that the cause of mutilation was postmortem scavenging by red foxes ( Vulpes vulpes). The probable cause of death was established in 26/32 (81%) carcasses: 10 were predated, 8 died from cardiorespiratory failure, 6 from blunt force trauma, one from ethylene glycol toxicity, and another from liver failure. In 6 carcasses a cause of death was not established due to autolysis and/or extensive mutilation. In summary, this study highlights the value of a multidisciplinary approach to fully investigate cases of suspected human-inflicted mutilation of animals.