scholarly journals A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells

2020 ◽  
Vol 27 (1) ◽  
pp. 181-185 ◽  
Author(s):  
Haibin Xi ◽  
Justin Langerman ◽  
Shan Sabri ◽  
Peggie Chien ◽  
Courtney S. Young ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Human Skeletal Muscle ◽  
Human Pluripotent Stem Cells ◽  
Stem And Progenitor Cells

scholarly journals A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells

2020 ◽  
Vol 27 (1) ◽  
pp. 158-176.e10 ◽  
Author(s):  
Haibin Xi ◽  
Justin Langerman ◽  
Shan Sabri ◽  
Peggie Chien ◽  
Courtney S. Young ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Human Skeletal Muscle ◽  
Human Pluripotent Stem Cells ◽  
Stem And Progenitor Cells

scholarly journals Transcriptional landscape of myogenesis from human pluripotent stem cells reveals a key role of TWIST1 in maintenance of skeletal muscle progenitors

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
In Young Choi ◽  
Hotae Lim ◽  
Hyeon Jin Cho ◽  
Yohan Oh ◽  
Bin-Kuan Chou ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Expression Profiles ◽  
Human Pluripotent Stem Cells ◽  
Knock Out ◽  
Muscle Progenitor Cells ◽  
Transcriptional Landscape

Generation of skeletal muscle cells with human pluripotent stem cells (hPSCs) opens new avenues for deciphering essential, but poorly understood aspects of transcriptional regulation in human myogenic specification. In this study, we characterized the transcriptional landscape of distinct human myogenic stages, including OCT4::EGFP+ pluripotent stem cells, MSGN1::EGFP+ presomite cells, PAX7::EGFP+ skeletal muscle progenitor cells, MYOG::EGFP+ myoblasts, and multinucleated myotubes. We defined signature gene expression profiles from each isolated cell population with unbiased clustering analysis, which provided unique insights into the transcriptional dynamics of human myogenesis from undifferentiated hPSCs to fully differentiated myotubes. Using a knock-out strategy, we identified TWIST1 as a critical factor in maintenance of human PAX7::EGFP+ putative skeletal muscle progenitor cells. Our data revealed a new role of TWIST1 in human skeletal muscle progenitors, and we have established a foundation to identify transcriptional regulations of human myogenic ontogeny (online database can be accessed in http://www.myogenesis.net/).

scholarly journals Haematopoietic stem and progenitor cells from human pluripotent stem cells

Nature ◽  
2017 ◽  
Vol 545 (7655) ◽  
pp. 432-438 ◽  
Author(s):  
Ryohichi Sugimura ◽  
Deepak Kumar Jha ◽  
Areum Han ◽  
Clara Soria-Valles ◽  
Edroaldo Lummertz da Rocha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Stem And Progenitor Cells
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