This study discusses the synthesis, characterization, and activity of silver nanoparticles, along with silver graphene oxide, against pathogenic bacteria. Silver nanoparticles are prepared by laser ablation and fabricated with silver graphene oxide. The synthesized silver nanoparticles and silver graphene oxide were characterized through UV-Vis spectrophotometer, Fourier-transforms spectroscopy, and zeta potential. The antimicrobial performance of silver nanoparticles and silver graphene oxide nanoparticles were monitored by the contradiction of gram-negative bacteria (Escherichia.coli and Acenetobacter bumannii), and gram positive bacteria: Staphylococcus aureus. Different concentrations of silver and silver graphene oxide have been investigated to monitor their antibacterial activity against bacterial isolates. The results indicate that the nanocomposites have antibacterial activity and the antibacterial effect increases with increased concentration of nanocomposites. Laser ablation method, a fast synthesis technique, has been used in the current study to prepare silver nanoparticle and silver nanoparticle-graphene oxide nanocomposites.