Kinetic resolution of 5-(hydroxymethyl)-3-phenyl-2-isoxazoline by using the ‘low-temperature method’ with porous ceramic-immobilized lipase

2005 ◽  
Vol 16 (8) ◽  
pp. 1535-1539 ◽  
Author(s):  
Takashi Sakai ◽  
Hiroshi Mitsutomi ◽  
Toshinobu Korenaga ◽  
Tadashi Ema
Keyword(s):  
Low Temperature ◽  
Kinetic Resolution ◽  
Immobilized Lipase ◽  
Porous Ceramic ◽  
Temperature Method

scholarly journals Validity of the digitonin method for metabolite compartmentation in isolated hepatocytes

1980 ◽  
Vol 188 (1) ◽  
pp. 207-212 ◽  
Author(s):  
D G Brocks ◽  
E A Siess ◽  
O H Wieland
Keyword(s):  
Low Temperature ◽  
Adenine Nucleotides ◽  
Isolated Hepatocytes ◽  
Cell Fractionation ◽  
Conventional Procedure ◽  
Cellular Content ◽  
Temperature Method

1. A modification of the digitonin method of Zuurendonk & Tager (1974) (Biochim. Biophys. Acta 333, 393-399) (i.e. the ‘convaentional’ method) was developed that allows the fractionation of isolated hepatocytes at −5 degrees C (i.e. ‘low-temperature’ method). 2. With respect to compartmentation of adenine nucleotides, glutamate and citrate, the two methods yielded very similar results. 3. In contrast, the mitochondrial amounts of aspartate and malate, as revealed by the low-temperature method, were about twice as high as those found by the conventional procedure. No change in the total cellular content occurred. 4. With n-butylmalonate and glisoxepid present in the conventional digitonin medium, significantly higher amounts of malate and aspartate respectively were found in the mitochondrial pellets. The results obtained by the low-temperature method, however, were not influenced by the these inhibitors. 5. It is concluded that under the conventional conditions of cell fractionation no appreciable redistribution of adenine nucleotides, glutamate and citrate occurs.

A useful low temperature method for post-embedding electron immunocytochemistry in routine histopathology

1987 ◽  
Vol 151 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Graham D. McPhail ◽  
Teresa Finn ◽  
Peter G. Isaacson
Keyword(s):  
Low Temperature ◽  
Temperature Method ◽  
Electron Immunocytochemistry

RETRACTED: Low temperature method for synthesis of ZnS quantum dots and its luminescence characterization studies

2013 ◽  
Vol 264 ◽  
pp. 17-20 ◽  
Author(s):  
K. Senthilkumar ◽  
T. Kalaivani ◽  
S. Kanagesan ◽  
V. Balasubramanian
Keyword(s):  
Quantum Dots ◽  
Low Temperature ◽  
Temperature Method ◽  
Characterization Studies

scholarly journals Micro-morphological Characterization of Fresh Cannabis sativa L. Roots After Lead Exposure Using Low Temperature Method for ESEM

2018 ◽  
Vol 24 (S1) ◽  
pp. 1306-1307
Author(s):  
Vilem Nedela ◽  
Eva Tihlafikova ◽  
Biljana Dordevic ◽  
Nikola Slamova
Keyword(s):  
Low Temperature ◽  
Lead Exposure ◽  
Cannabis Sativa ◽  
Morphological Characterization ◽  
Temperature Method
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