A useful low temperature method for post-embedding electron immunocytochemistry in routine histopathology

1987 ◽  
Vol 151 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Graham D. McPhail ◽  
Teresa Finn ◽  
Peter G. Isaacson
Keyword(s):  
Low Temperature ◽  
Temperature Method ◽  
Electron Immunocytochemistry

scholarly journals Validity of the digitonin method for metabolite compartmentation in isolated hepatocytes

1980 ◽  
Vol 188 (1) ◽  
pp. 207-212 ◽  
Author(s):  
D G Brocks ◽  
E A Siess ◽  
O H Wieland
Keyword(s):  
Low Temperature ◽  
Adenine Nucleotides ◽  
Isolated Hepatocytes ◽  
Cell Fractionation ◽  
Conventional Procedure ◽  
Cellular Content ◽  
Temperature Method

1. A modification of the digitonin method of Zuurendonk & Tager (1974) (Biochim. Biophys. Acta 333, 393-399) (i.e. the ‘convaentional’ method) was developed that allows the fractionation of isolated hepatocytes at −5 degrees C (i.e. ‘low-temperature’ method). 2. With respect to compartmentation of adenine nucleotides, glutamate and citrate, the two methods yielded very similar results. 3. In contrast, the mitochondrial amounts of aspartate and malate, as revealed by the low-temperature method, were about twice as high as those found by the conventional procedure. No change in the total cellular content occurred. 4. With n-butylmalonate and glisoxepid present in the conventional digitonin medium, significantly higher amounts of malate and aspartate respectively were found in the mitochondrial pellets. The results obtained by the low-temperature method, however, were not influenced by the these inhibitors. 5. It is concluded that under the conventional conditions of cell fractionation no appreciable redistribution of adenine nucleotides, glutamate and citrate occurs.

RETRACTED: Low temperature method for synthesis of ZnS quantum dots and its luminescence characterization studies

2013 ◽  
Vol 264 ◽  
pp. 17-20 ◽  
Author(s):  
K. Senthilkumar ◽  
T. Kalaivani ◽  
S. Kanagesan ◽  
V. Balasubramanian
Keyword(s):  
Quantum Dots ◽  
Low Temperature ◽  
Temperature Method ◽  
Characterization Studies

scholarly journals Micro-morphological Characterization of Fresh Cannabis sativa L. Roots After Lead Exposure Using Low Temperature Method for ESEM

2018 ◽  
Vol 24 (S1) ◽  
pp. 1306-1307
Author(s):  
Vilem Nedela ◽  
Eva Tihlafikova ◽  
Biljana Dordevic ◽  
Nikola Slamova
Keyword(s):  
Low Temperature ◽  
Lead Exposure ◽  
Cannabis Sativa ◽  
Morphological Characterization ◽  
Temperature Method

scholarly journals A low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis

1991 ◽  
Vol 280 (2) ◽  
pp. 331-334 ◽  
Author(s):  
N Flint ◽  
F L Cove ◽  
G S Evans
Keyword(s):  
Small Intestine ◽  
Low Temperature ◽  
Intestinal Epithelium ◽  
Sequential Fractionation ◽  
Potential Value ◽  
Temperature Method ◽  
Small Intestinal ◽  
The Comparative Study ◽  
Cell Phenotypes ◽  
Proliferative Cell

A variety of enzymic and non-enzymic methods to isolate epithelium from the small intestine have been previously published. Sequential fractionation of cells from the villus to the crypt has been reported in some of these papers, which allows the comparative study of terminally differentiated and proliferative cell phenotypes. However, these methods often involve the incubation of tissues at 37 degrees C, which may affect the structural and biochemical integrity of the cells. We have developed a rapid low-temperature (4 degrees C) method for isolating purified populations of crypt and villus cells from mouse and rat intestines. The fractionated cells have been partially characterized, and the potential value of the procedure has been indicated by the ability to analyse the comparative protein and mRNA expression along the crypt-villus axis.

Structural and optical characterization of hemimorphite with flower-like morphology synthesized by a novel low-temperature method

2012 ◽  
Vol 85 ◽  
pp. 138-141 ◽  
Author(s):  
Ivana Lj. Validžić ◽  
Miodrag Mitrić ◽  
Bojan M. Jokić ◽  
Mirjana I. Čomor
Keyword(s):  
Low Temperature ◽  
Optical Characterization ◽  
Temperature Method

The low temperature method for environmental scanning electron microscopy – a new method for observation of diatom assemblages in vivo

2018 ◽  
Vol 33 (3) ◽  
pp. 397-403 ◽  
Author(s):  
Markéta Fránková ◽  
Aloisie Poulíčková ◽  
Vilém Neděla ◽  
Eva Tihlaříková ◽  
Kateřina Šumberová ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Low Temperature ◽  
Environmental Scanning Electron Microscopy ◽  
New Method ◽  
Environmental Scanning ◽  
Diatom Assemblages ◽  
Temperature Method ◽  
Scanning Electron

Arsenite remediation by an amine-rich graphitic carbon nitride synthesized by a novel low-temperature method

2014 ◽  
Vol 256 ◽  
pp. 347-355 ◽  
Author(s):  
C. Daikopoulos ◽  
Y. Georgiou ◽  
A.B. Bourlinos ◽  
M. Baikousi ◽  
M.A. Karakassides ◽  
...  
Keyword(s):  
Low Temperature ◽  
Carbon Nitride ◽  
Graphitic Carbon ◽  
Graphitic Carbon Nitride ◽  
Temperature Method
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