Epidermal growth factor can be used in lieu of follicle-stimulating hormone for nuclear maturation of porcine oocytes in vitro

2010 ◽  
Vol 73 (8) ◽  
pp. 1024-1036 ◽  
Author(s):  
S.J. Uhm ◽  
M.K. Gupta ◽  
J.H. Yang ◽  
H.-J. Chung ◽  
T.S. Min ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Follicle Stimulating Hormone ◽  
Nuclear Maturation ◽  
Epidermal Growth ◽  
Stimulating Hormone

In vitro effects of epidermal growth factor, follicle stimulating hormone and testosterone on mouse spermatogonial cell colony formation

2006 ◽  
Vol 18 (6) ◽  
pp. 709 ◽  
Author(s):  
S. Hadi Anjamrooz ◽  
Mansoureh Movahedin ◽  
Taki Tiraihi ◽  
S. Javad Mowla
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Colony Formation ◽  
Follicle Stimulating Hormone ◽  
Spermatogonial Cell ◽  
Epidermal Growth ◽  
In Vitro Effects ◽  
Cell Colony ◽  
Stimulating Hormone

The complex process of spermatogenesis is regulated by various factors. In the present study, the in vitro effects of epidermal growth factor (EGF), follicle stimulating hormone (FSH) and testosterone on spermatogonial cell colony formation were investigated, and the best colonising factor was chosen for treating cells before transplantation. Sertoli and spermatogonial cells were isolated from neonatal mouse testes. The identity of the cells was confirmed through analysis of morphology, alkaline phosphatase activity, immunoreactivity and transplantation. Co-cultured Sertoli and spermatogonial cells were treated with EGF, FSH and testosterone before colony assay. Results indicated that EGF is the best factor for in vitro colonisation of spermatogonial cells, but transplantation of the EGF-treated group did not show any significant change compared with the control groups. In conclusion, EGF increased in vitro colonisation of spermatogonial cells, but, as a result of differential effects, did not influence transplantation efficiency.

Synergetic effects of epidermal growth factor and estradiol on cytoplasmic maturation of porcine oocytes

Zygote ◽  
2002 ◽  
Vol 10 (4) ◽  
pp. 349-354 ◽  
Author(s):  
Yong-Hai Li ◽  
Rui-Hua Liu ◽  
Li-Hong Jiao ◽  
Wei-Hua Wang
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Early Embryo ◽  
Antral Follicles ◽  
Nuclear Maturation ◽  
17Β Estradiol ◽  
Epidermal Growth ◽  
Cytoplasmic Maturation ◽  
Pronuclear Formation

This study was conducted to examine the effect of epidermal growth factor (EGF) and 17β-estradiol (E2) on nuclear and cytoplasmic (male pronuclear formation and early embryo development) maturation of porcine oocytes. Oocytes were aspirated from antral follicles and cultured in modified TCM-199 medium supplemented with 0.57 mM cysteine, 10 IU/ml eCG, 10 IU/ml hCG, with or without EGF and/or E2. In vitro fertilisation of matured oocytes was performed in a modified Tris-buffered medium (mTBM) with frozen-thawed ejaculated spermatozoa. Oocytes were transferred to NCSU-23 supplemented with 0.4% bovine serum albumin at 6 h after in vitro fertilisation. Significantly higher (p < 0.05) rates of nuclear maturation, pronuclear formation and cleavage (91.7%, 65.2% and 37.3%, respectively) were observed when oocytes were cultured in the medium containing both EGF (10 ng/ml) and E2 (1 μg/ml) than in the medium supplemented with either EGF or E2 or without both. Intracellular glutathione concentration in the oocytes cultured in the medium containing both E2 and EGF was also significantly higher (12.1 pmol per oocyte) than that of oocytes cultured in the medium with E2 or EGF alone or without both. These findings suggested that EGF and E2 have a synergestic effect on both nuclear and cytoplasmic maturation of porcine oocytes.

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