scholarly journals Antisense epidermal growth factor oligodeoxynucleotides inhibit follicle-stimulating hormone-induced in vitro DNA and progesterone synthesis in hamster preantral follicles.

1994 ◽  
Vol 8 (9) ◽  
pp. 1175-1181
Author(s):  
S K Roy ◽  
S G Harris
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Follicle Stimulating Hormone ◽  
Preantral Follicles ◽  
Progesterone Synthesis ◽  
Epidermal Growth ◽  
Stimulating Hormone

In vitro effects of epidermal growth factor, follicle stimulating hormone and testosterone on mouse spermatogonial cell colony formation

2006 ◽  
Vol 18 (6) ◽  
pp. 709 ◽  
Author(s):  
S. Hadi Anjamrooz ◽  
Mansoureh Movahedin ◽  
Taki Tiraihi ◽  
S. Javad Mowla
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Colony Formation ◽  
Follicle Stimulating Hormone ◽  
Spermatogonial Cell ◽  
Epidermal Growth ◽  
In Vitro Effects ◽  
Cell Colony ◽  
Stimulating Hormone

The complex process of spermatogenesis is regulated by various factors. In the present study, the in vitro effects of epidermal growth factor (EGF), follicle stimulating hormone (FSH) and testosterone on spermatogonial cell colony formation were investigated, and the best colonising factor was chosen for treating cells before transplantation. Sertoli and spermatogonial cells were isolated from neonatal mouse testes. The identity of the cells was confirmed through analysis of morphology, alkaline phosphatase activity, immunoreactivity and transplantation. Co-cultured Sertoli and spermatogonial cells were treated with EGF, FSH and testosterone before colony assay. Results indicated that EGF is the best factor for in vitro colonisation of spermatogonial cells, but transplantation of the EGF-treated group did not show any significant change compared with the control groups. In conclusion, EGF increased in vitro colonisation of spermatogonial cells, but, as a result of differential effects, did not influence transplantation efficiency.

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