Roscovitine is a novel agent that can be used for the activation of porcine oocytes reconstructed with adult cutaneous or fetal fibroblast cell nuclei

2012 ◽  
Vol 78 (8) ◽  
pp. 1855-1867 ◽  
Author(s):  
M. Samiec ◽  
M. Skrzyszowska
Keyword(s):  
Fibroblast Cell ◽  
Cell Nuclei ◽  
Fetal Fibroblast ◽  
Novel Agent ◽  
Fetal Fibroblast Cell

scholarly journals Production of a cloned calf from a fetal fibroblast cell line

2003 ◽  
Vol 36 (11) ◽  
pp. 1485-1489 ◽  
Author(s):  
M.R.B. Mello ◽  
H.V.A. Caetano ◽  
M.G. Marques ◽  
M.S. Padilha ◽  
J.F. Garcia ◽  
...  
Keyword(s):  
Cell Line ◽  
Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Fetal Fibroblast ◽  
Fetal Fibroblast Cell

scholarly journals Differences in Glycolysis Between Fetal and Non-fetal Fibroblast Cell Lines

1970 ◽  
Vol 4 (6) ◽  
pp. 511-511
Author(s):  
Antoinette Condon ◽  
Frank A Oski ◽  
Salvatore DiMauro ◽  
William J Mellman
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Fetal Fibroblast ◽  
Fetal Fibroblast Cell ◽  
Fibroblast Cell Lines

scholarly journals Establishment, Culture, and Characterization of Guinea Pig Fetal Fibroblast Cell

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Davood Mehrabani ◽  
Reza Mahboobi ◽  
Mehdi Dianatpour ◽  
Shahrokh Zare ◽  
Amin Tamadon ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Culture Method ◽  
Fibroblast Cell ◽  
Biological Evaluation ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Average Cell ◽  
Fetal Fibroblast ◽  
Cell Counts ◽  
Fetal Fibroblast Cell

Establishment of Guinea pig fetal fibroblast cells and their biological evaluation before and after cryopreservation were the main purposes of this study. After determination of the proper age of pregnancy by ultrasonography, 30 days old fetuses of Guinea pigs were recovered. Their skins were cut into small pieces (1 mm2) and were cultured. When reaching 80–90% confluence, the cells were passaged. Cells of the second and eighth passages were cultured in 24-well plates (4×104cells/well) for 6 days and three wells per day were counted. The average cell counts at each time point were then plotted against time and the population doubling time (PDT) was determined. Then, vials of cells (2×106cells/mL) were cryopreserved for 1 month and after thawing, the cell viability was evaluated. The PDT of the second passage was about 23 h and for the eighth passage was about 30 h. The viability of the cultures was 95% in the second passage and 74.5% in the eighth passage. It was shown that the Guinea pig fetal fibroblast cell culture can be established using the adherent culture method while, after freezing, the viability indices of these cells were favorable.

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