Hydroxyl radical production via NADPH oxidase in rat striatum due to carbon monoxide poisoning

Toxicology ◽  
2018 ◽  
Vol 394 ◽  
pp. 63-71 ◽  
Author(s):  
Shuichi Hara ◽  
Masamune Kobayashi ◽  
Fumi Kuriiwa ◽  
Kazuya Ikematsu ◽  
Hajime Mizukami
Keyword(s):  
Carbon Monoxide ◽  
Nadph Oxidase ◽  
Hydroxyl Radical ◽  
Carbon Monoxide Poisoning ◽  
Rat Striatum ◽  
Radical Production

Role of nitric oxide system in hydroxyl radical generation in rat striatum due to carbon monoxide poisoning, as determined by microdialysis

Toxicology ◽  
2007 ◽  
Vol 239 (1-2) ◽  
pp. 136-143 ◽  
Author(s):  
Shuichi Hara ◽  
Toshiji Mukai ◽  
Kunihiko Kurosaki ◽  
Hajime Mizukami ◽  
Fumi Kuriiwa ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Carbon Monoxide ◽  
Hydroxyl Radical ◽  
Carbon Monoxide Poisoning ◽  
Oxide System ◽  
Rat Striatum ◽  
Radical Generation ◽  
Nitric Oxide System

Existence of a threshold for hydroxyl radical generation independent of hypoxia in rat striatum during carbon monoxide poisoning

2011 ◽  
Vol 85 (9) ◽  
pp. 1091-1099 ◽  
Author(s):  
Shuichi Hara ◽  
Hajime Mizukami ◽  
Kunihiko Kurosaki ◽  
Fumi Kuriiwa ◽  
Toshiji Mukai
Keyword(s):  
Carbon Monoxide ◽  
Hydroxyl Radical ◽  
Carbon Monoxide Poisoning ◽  
Rat Striatum ◽  
Radical Generation

Involvement of extracellular ascorbate and iron in hydroxyl radical generation in rat striatum in carbon monoxide poisoning

Toxicology ◽  
2009 ◽  
Vol 264 (1-2) ◽  
pp. 69-73 ◽  
Author(s):  
Shuichi Hara ◽  
Hajime Mizukami ◽  
Toshiji Mukai ◽  
Kunihiko Kurosaki ◽  
Fumi Kuriiwa ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Hydroxyl Radical ◽  
Carbon Monoxide Poisoning ◽  
Rat Striatum ◽  
Radical Generation

Eff ects of hyperbaric oxygen on NLRP3 infl ammasome activation in the brain aft er carbon monoxide poisoning

2020 ◽  
pp. 607-619
Author(s):  
Ya’nan Qi ◽  
◽  
Zhibao Guo ◽  
Huijun Hu ◽  
Xiang’en Meng ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Nadph Oxidase ◽  
Hyperbaric Oxygen ◽  
Nlrp3 Inflammasome ◽  
Carbon Monoxide Poisoning ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammasome Activation ◽  
Protein Levels ◽  
Nlrp3 Inflammasome Activation ◽  
Myelin Injury

Neuroinflammation plays an important role in brain damage after acute carbon monoxide poisoning (ACOP). The nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing (NLRP) 3 inflammasome triggers the activation of inflammatory caspases and maturation of interleukin (IL)-1β and -18, and has been linked to various human autoinflammatory and autoimmune diseases. In this study we investigated the effects of hyperbaric oxygen (HBO2) on NLRP3 inflammasome activation after ACOP. Mice were randomly divided into four groups: sham group (exposure to normobaric air – i.e., 21% O2 at 1 atmosphere absolute); HBO2-only group; CO + normobaric air group; and CO + HBO2 group. Cognitive function was evaluated with the Morris water maze; myelin injury was assessed by Fluoro-Myelin GreenTM fluorescent myelin staining and myelin basic protein (MBP) immunostaining; and mRNA and protein levels of NLRP3 inflammasome complex proteins were measured by quantitative real-time PCR and Western blot, respectively. Additionally, serum and brain levels of IL-1β and -18 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were determined by enzyme-linked immunosorbent assay. It was found that HBO2 improved learning and memory, and alleviated myelin injury in mice subjected to acute CO exposure. Furthermore, HBO2 decreased NLRP3, absent in melanoma 2 (AIM2), caspase-1, and apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain mRNA and protein levels, and reduced brain and serum concentrations of IL-1β and -18 and NADPH oxidase. These results indicate that HBO2 suppresses the inflammatory response after ACOP by blocking NLRP3 inflammasome activation, thereby alleviating cognitive deficits.

Characterization of hydroxyl radical generation in the striatum of free-moving rats due to carbon monoxide poisoning, as determined by in vivo microdialysis

2004 ◽  
Vol 1016 (2) ◽  
pp. 281-284 ◽  
Author(s):  
Shuichi Hara ◽  
Toshiji Mukai ◽  
Kunihiko Kurosaki ◽  
Fumi Kuriiwa ◽  
Takahiko Endo
Keyword(s):  
Carbon Monoxide ◽  
Hydroxyl Radical ◽  
Carbon Monoxide Poisoning ◽  
In Vivo Microdialysis ◽  
Radical Generation

Dual contradictory roles of cAMP signaling pathways in hydroxyl radical production in the rat striatum

2012 ◽  
Vol 52 (6) ◽  
pp. 1086-1092 ◽  
Author(s):  
Shuichi Hara ◽  
Masamune Kobayashi ◽  
Fumi Kuriiwa ◽  
Toshiji Mukai ◽  
Hajime Mizukami
Keyword(s):  
Hydroxyl Radical ◽  
Signaling Pathways ◽  
Camp Signaling ◽  
Rat Striatum ◽  
Radical Production
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