In Vitro Impact of Pro-Senescent Endothelial Microvesicles on Isolated Pancreatic Rat Islets Function

2021 ◽  
Author(s):  
Mohamad Kassem ◽  
Ali El Habhab ◽  
Guillaume Kreutter ◽  
Lamia Amoura ◽  
Philippe Baltzinger ◽  
...  
Keyword(s):  
Rat Islets

In vitro activation of mice splenocytes by free and encapsulated rat islets and by components of capsular wall

2002 ◽  
Vol 34 (2) ◽  
pp. 659-660 ◽  
Author(s):  
E Godlewska ◽  
E Sitarek ◽  
M Iwańska ◽  
T Orlowski
Keyword(s):  
Rat Islets ◽  
In Vitro Activation

Absence of toxicity associated with adenoviral-mediated transfer of the β-galactosidase reporter gene to neonatal rat islets in vitro

1999 ◽  
Vol 44 (3) ◽  
pp. 157-163 ◽  
Author(s):  
Timothy O’Brien ◽  
Allan E. Karlsen ◽  
Henrik U. Andersen ◽  
Thomas Mandrup-Poulsen ◽  
Jørn Nerup
Keyword(s):  
Reporter Gene ◽  
Neonatal Rat ◽  
Rat Islets

Low concentrations of interleukin-1 stimulate and high concentrations inhibit insulin release from isolated rat islets of Langerhans

1986 ◽  
Vol 113 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Giatgen A. Spinas ◽  
Thomas Mandrup-Poulsen ◽  
Jens Mølvig ◽  
Leif Bæk ◽  
Klaus Bendtzen ◽  
...  
Keyword(s):  
Insulin Release ◽  
Interleukin 1 ◽  
Insulin Biosynthesis ◽  
Rat Islets ◽  
Low Concentrations ◽  
Isolated Rat Islets ◽  
High Concentrations ◽  
Rat Islets Of Langerhans ◽  
Isolated Rat

Abstract. Isolated rat islets were incubated either with crude, affinity-purified or recombinant human interleukin-1 for 1 to 6 days. A significant (20–60%) increase of insulin release was observed at low concentrations of all three interleukin-1-containing preparations. In contrast, higher concentrations dose-dependently inhibited the insulin release. The increased insulin secretion occurred at concentrations below those necessary to augment the mitogen response to phytohaemagglutinin of murine thymocytes in vitro. These doses (0.05-0.5 U/ml) correspond to 0.2-2 ng of recombinant interleukin-1 per ml, equal to approximately 0.01-0.1 pmol/ml. In doses of 0.6-1.8 U/ml affinitypurified interleukin-1 significantly increased the islet insulin content per ng of DNA, indicating a stimulation of insulin-biosynthesis. The data support the concept that low concentrations of interleukin-1 may play a role in priming the physiological secretion of insulin.

REPRODUCIBLE HIGH YIELD OF RAT ISLETS BY STATIONARY IN VITRO DIGESTION FOLLOWING PANCREATIC DUCTAL OR PORTAL VENOUS COLLAGENASE INJECTION1

1987 ◽  
Vol 43 (5) ◽  
pp. 725-730 ◽  
Author(s):  
MITSUKAZU GOTOH ◽  
TAKASHI MAKI ◽  
SUSUMU SATOMI ◽  
JANIS PORTER ◽  
SUSAN BONNER-WEIR ◽  
...  
Keyword(s):  
In Vitro Digestion ◽  
High Yield ◽  
Rat Islets

Effects of encapsulation on in vitro function of cryopreserved rat islets

2002 ◽  
Vol 34 (2) ◽  
pp. 651-652 ◽  
Author(s):  
M Sabat ◽  
E Godlewska ◽  
E Sitarek ◽  
T Orłowski
Keyword(s):  
Rat Islets
Sign in / Sign up

Export Citation Format

Share Document