Use of enoyl coenzyme A hydratase of Mycobacterium avium subsp. paratuberculosis for the serological diagnosis of Johne's disease

2013 ◽  
Vol 155 (4) ◽  
pp. 253-258 ◽  
Author(s):  
Reiko Nagata ◽  
Satoko Kawaji ◽  
Yasuyuki Mori
Keyword(s):  
Mycobacterium Avium ◽  
Coenzyme A ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Serological Diagnosis ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals The collection of lymphatic fluid from the bovine udder and its use for the detection of Mycobacterium avium subsp. paratuberculosis in the cow

2011 ◽  
Vol 24 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Johannes L. Khol ◽  
Pablo J. Pinedo ◽  
Claus D. Buergelt ◽  
Laura M. Neumann ◽  
Walter Baumgartner ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Fluid Collection ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lymph Fluid ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Lymphatic Fluid ◽  
Elisa Result

The objective of the current study was to evaluate the feasibility of lymph collection from the bovine udder and to investigate if the lymphatic fluid might be of diagnostic value in cows infected with Mycobacterium avium subsp. paratuberculosis (MAP), the etiologic agent of paratuberculosis. Lymph fluid collection was attempted from 58 cows, and the reactions of the cows as well as the level of difficulty of the procedure were recorded in 56 animals. Lymph samples (51 in total) were tested for the presence of MAP by nested polymerase chain reaction. Collection of the lymphatic fluid caused no or mild signs of discomfort in 94.6% of the cows; in 51.8% of cows, lymphatic fluid was attained on the first attempt, while sample collection was unsuccessful in 12.1%. Mycobacterium avium subsp. paratuberculosis was detected in 43.1% of all lymph samples. The bacterium was present in 66.7% of cows with clinical Johne’s disease, in 42.8% of asymptomatic cows with a positive or suspicious enzyme-linked immunosorbent assay (ELISA) result in blood, and in 38.7% of cows with a negative ELISA result in blood. The present study shows that the procedure was well tolerated by most cows and can easily be performed on farm. The current report of the isolation of MAP from lymph fluid suggests that the present approach could be used for the early detection of Johne’s disease in cattle.

scholarly journals Complete Genome Sequence of the Telford Type S Strain of Mycobacterium avium subsp. paratuberculosis

2019 ◽  
Vol 8 (11) ◽  
Author(s):  
Rudiger Brauning ◽  
Karren Plain ◽  
Milan Gautam ◽  
Tonia Russell ◽  
C. Carolina Correa ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Mycobacterium Avium ◽  
Causative Agent ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Content Type ◽  
Mycobacterium Avium Subsp Paratuberculosis

Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne’s disease (JD).

scholarly journals Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein of Mycobacterium avium subsp. paratuberculosis

2007 ◽  
Vol 14 (3) ◽  
pp. 312-317 ◽  
Author(s):  
John P. Bannantine ◽  
Thomas J. Radosevich ◽  
Judith R. Stabel ◽  
Sven Berger ◽  
J. Frank T. Griffin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Membrane Protein ◽  
Mycobacterium Avium ◽  
Epitope Mapping ◽  
Immunofluorescence Microscopy ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis

ABSTRACT The Mycobacterium avium subsp. paratuberculosis 35-kDa major membrane protein (MMP) encoded by MAP2121c is an important membrane antigen recognized in cattle with Johne's disease. In this study, purified recombinant MMP was used to produce two stable monoclonal antibodies, termed 8G2 and 13E1, which were characterized by immunoblotting, epitope mapping, and immunofluorescence microscopy.

Sign in / Sign up

Export Citation Format

Share Document