Relationship between increased albendazole systemic exposure and changes in single nucleotide polymorphisms on the β-tubulin isotype 1 encoding gene in Haemonchus contortus

Background and Aim: Haemonchus contortus is one of the major trichostrongyloid nematodes affecting small ruminant production worldwide, especially in tropical and subtropical regions. Adult H. contortus suck the blood from the host abomasum leading to anemia and often death in heavily infected animals. The mainstay of parasitic control is an anthelmintic drug, but long-term drug use may cause drug resistance. The aim of this study was to examine benzimidazole resistance in H. contortus of goats from different regions in Thailand by detecting the frequency of the F200Y polymorphism in the β-tubulin isotype 1 gene. Materials and Methods: A total of 121 H. contortus adults were obtained from 31 naturally infected out of 37 slaughtered goats from city abattoirs in five regions of Thailand. The frequency of the F200Y polymorphism in the β-tubulin isotype 1 gene was detected following the allele-specific polymerase chain reaction protocol. Results: The overall genotype frequencies in Thailand were homozygous resistant (RR: 24%), heterozygous (SR: 44.6%), and homozygous susceptible (SS: 31.4%). The allele frequencies were resistant allele (R: 46%) and susceptible allele (S: 54%). The R allele frequency and the RR genotype varied from 30% to 65% and 0% to 43.9%, respectively. The frequency of R alleles was significantly higher in the southern region (0.65) as compared to northern (0.30, p=0.001), western (0.38, p=0.04), and central regions (0.30, p=0.03). The RR genotype was also significantly higher in the southern region (43.9%) versus the northern (0 %, p=0.001), western (11.8%, p=0.012), and central regions (17.4%, p=0.001). Conclusion: This is the first study of the detection of single-nucleotide polymorphisms in codon 200 of the β-tubulin isotype 1 gene of H. contortus from goats in Thailand. These findings are essential and imply that an integrated approach is needed for issues such as drug treatment, farm management, prevention, and control strategies. This is of interest to farmers, veterinarians, and the department of livestock.

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Single nucleotide polymorphism (SNP) markers for benzimidazole resistance in veterinary nematodes

Parasitology ◽

10.1017/s0031182007000054 ◽

2007 ◽

Vol 134 (8) ◽

pp. 1077-1086 ◽

Cited By ~ 76

Author(s):

G. VON SAMSON-HIMMELSTJERNA ◽

W. J. BLACKHALL ◽

J. S. McCARTHY ◽

P. J. SKUCE

Keyword(s):

Single Nucleotide Polymorphisms ◽

Point Mutations ◽

Building Blocks ◽

Snp Markers ◽

Nucleotide Polymorphisms ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Principal Mechanism ◽

Tubulin Isotype ◽

Veterinary Importance

SUMMARYResistance to the benzimidazole class of anthelmintics in nematodes of veterinary importance has a long history. Research into the mechanisms responsible for this resistance is subsequently at a more advanced stage than for other classes of anthelmintics. The principal mechanism of resistance to benzimidazoles is likely to involve changes in the primary structure of β-tubulins, the building blocks of microtubules. Specifically, point mutations in the β-tubulin isotype 1 gene leading to amino acid substitutions in codons 167, 198, and 200 of the protein have been associated with resistance in nematodes. These single nucleotide polymorphisms offer a means of detecting the presence of resistance within populations. In this mini-review, we focus on the prevalence and importance of these polymorphisms in three groups of nematodes: trichostrongylids, cyathostomins, and hookworms. A brief overview of existing strategies for genotyping single nucleotide polymorphisms is also presented. The CARS initiative hopes to exploit these known polymorphisms to further our understanding of the phenomenon of BZ resistance.

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Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Journal of Animal Science and Biotechnology ◽

10.1186/s40104-019-0327-8 ◽

2019 ◽

Vol 10 (1) ◽

Cited By ~ 2

Author(s):

Mahmuda Bilkis Bintee Alam ◽

Abdullah Ibne Omar ◽

Md. Omar Faruque ◽

David Russell Notter ◽

Kathiravan Periasamy ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Candidate Genes ◽

Haemonchus Contortus ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Single nucleotide polymorphisms in β-tubulin selected in Onchocerca volvulus following repeated ivermectin treatment: Possible indication of resistance selection

Molecular and Biochemical Parasitology ◽

10.1016/j.molbiopara.2012.05.005 ◽

2012 ◽

Vol 185 (1) ◽

pp. 10-18 ◽

Cited By ~ 17

Author(s):

Hugues Nana-Djeunga ◽

Catherine Bourguinat ◽

Sébastien D.S. Pion ◽

Joseph Kamgno ◽

Jacques Gardon ◽

...

Keyword(s):

Single Nucleotide Polymorphisms ◽

Onchocerca Volvulus ◽

Ivermectin Treatment ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Resistance Selection ◽

Β Tubulin

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Faculty Opinions recommendation of Single nucleotide polymorphisms in β-tubulin selected in Onchocerca volvulus following repeated ivermectin treatment: possible indication of resistance selection.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717953486.793459106 ◽

2012 ◽

Author(s):

Achim Hoerauf ◽

Kenneth Pfarr

Keyword(s):

Single Nucleotide Polymorphisms ◽

Onchocerca Volvulus ◽

Ivermectin Treatment ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Resistance Selection ◽

Β Tubulin

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SINGLE NUCLEOTIDE POLYMORPHISMS IN DRB1, IGF1 AND ILs ASSOCIATED WITH FECAL EGG COUNT CONFERS RESISTANCE AGAINST Haemonchus contortus INFECTION IN GOATS

The Pakistan Journal of Agricultural Sciences ◽

10.21162/pakjas/16.4854 ◽

2016 ◽

Vol 53 (04) ◽

pp. 963-970 ◽

Cited By ~ 2

Author(s):

Akhtar Rasool Asif

Keyword(s):

Single Nucleotide Polymorphisms ◽

Haemonchus Contortus ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Fecal Egg Count

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Chemokine C-C Motif Ligand 4 Gene Polymorphisms Associated with Susceptibility to Rheumatoid Arthritis

BioMed Research International ◽

10.1155/2018/9181647 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Shu-Jui Kuo ◽

Chien-Chung Huang ◽

Chun-Hao Tsai ◽

Horng-Chaung Hsu ◽

Chen-Ming Su ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Gene Expression ◽

Single Nucleotide Polymorphisms ◽

Protective Effect ◽

Gene Polymorphisms ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Encoding Gene

Chemokine C-C motif ligand 4 (CCL4) gene is a chemokine-encoding gene, and the polymorphism of CCL4 gene has been shown to predict risk of various diseases. We want to investigate whether the single nucleotide polymorphisms (SNPs) of the CCL4 gene can predict the risk of rheumatoid arthritis (RA). Between 2007 and 2015, we recruited 217 patients diagnosed with RA and 371 control participants. Comparative genotyping of the rs1634507, rs10491121, and rs1719153 SNPs was performed. When compared with participants with the A/A genotype of rs1719153, those with the A/T genotype were less likely to develop RA, as were those with the A/T+T/T genotype. The protective effect of the T-containing genotype was even more prominent among females. Those with A/T in rs1719153 were 56% less likely to develop RA compared with females with A/A; a similar protective effect was seen for females with the A/T+T/T genotype compared with those with A/A. The GTEx database revealed that patients carrying the T/T genotype had lower levels of CCL4 gene expression than those carrying the A/A genotype. These results indicate that the nucleotide T over the rs1719153 is associated with decreased CCL4 gene expression and decreased risk for RA.

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