scholarly journals Shrimp NF-κB binds to the immediate-early gene ie1 promoter of white spot syndrome virus and upregulates its activity

Virology ◽  
2010 ◽  
Vol 406 (2) ◽  
pp. 176-180 ◽  
Author(s):  
Xian-De Huang ◽  
Li Zhao ◽  
Hai-Qing Zhang ◽  
Xiao-Peng Xu ◽  
Xiao-Ting Jia ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Immediate Early Gene ◽  
Early Gene ◽  
Immediate Early ◽  
White Spot ◽  
White Spot Syndrome

scholarly journals Characterization of white spot syndrome virus immediate-early gene promoters

2013 ◽  
Vol 94 (2) ◽  
pp. 387-392 ◽  
Author(s):  
Fanyu Lin ◽  
He Huang ◽  
Wei Ke ◽  
Luhong Hou ◽  
Fang Li ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
White Spot Syndrome Virus ◽  
Initiation Site ◽  
Early Gene ◽  
Immediate Early ◽  
White Spot ◽  
Viral Gene ◽  
Gene Promoters ◽  
Promoter Regions ◽  
White Spot Syndrome

Twenty-one immediate-early (IE) genes of white spot syndrome virus (WSSV) have been identified so far. However, the transcriptional regulation of WSSV IE genes remains largely unknown. In this report, the 5′ flanking regions of 18 WSSV IE genes were cloned and eight functional promoter regions were identified. WSSV IE gene promoters normally contained a TATA box approximately 30 bp upstream of the transcriptional initiation site. Also, the cyclic AMP response element (CRE; TGACGTCA) was frequently found within the WSSV IE promoter regions. Mutations of the CREs of WSSV IE promoters P403 and P465 reduced their activity significantly, suggesting that these elements have a role in WSSV IE gene transcription. Our findings provide a more global view of WSSV IE gene promoters and will facilitate the in-depth investigation of viral gene transcriptional regulation.

scholarly journals Microarray and RT-PCR screening for white spot syndrome virus immediate-early genes in cycloheximide-treated shrimp

Virology ◽  
2005 ◽  
Vol 334 (2) ◽  
pp. 327-341 ◽  
Author(s):  
Wang-Jing Liu ◽  
Yun-Shiang Chang ◽  
Chung-Hsiung Wang ◽  
Guang-Hsiung Kou ◽  
Chu-Fang Lo
Keyword(s):  
White Spot Syndrome Virus ◽  
Immediate Early Genes ◽  
Immediate Early ◽  
White Spot ◽  
Rt Pcr ◽  
Pcr Screening ◽  
Early Genes ◽  
White Spot Syndrome

scholarly journals Identification of the thymidylate synthase within the genome of white spot syndrome virus

2004 ◽  
Vol 85 (7) ◽  
pp. 2035-2044 ◽  
Author(s):  
Qin Li ◽  
Deng Pan ◽  
Jing-hai Zhang ◽  
Feng Yang
Keyword(s):  
Thymidylate Synthase ◽  
White Spot Syndrome Virus ◽  
De Novo ◽  
Early Gene ◽  
White Spot ◽  
Binding Assays ◽  
Dna Viruses ◽  
Reading Frame ◽  
White Spot Syndrome ◽  
Eukaryotic Organisms

Thymidylate synthase (TS) (EC 2.1.1.45) is essential for the de novo synthesis of dTMP in prokaryotic and eukaryotic organisms. Within the white spot syndrome virus (WSSV) genome, an open reading frame (WSV067) that encodes a 289 amino acid polypeptide showed significant homology to all known TSs from species including mammals, plants, fungi, protozoa, bacteria and DNA viruses. In this study, WSV067 was expressed in Escherichia coli, and the purified recombinant protein showed TS activity in dUMP−folate-binding assays using ultraviolet difference spectroscopy. RT-PCR and Western blot analyses showed that WSV067 was a genuine and early gene. Phylogenetic analysis revealed that WSSV-TS was more closely related to the TSs of eukaryotes than to those from prokaryotes.

scholarly journals Identification of the immediate-early genes of white spot syndrome virus

Virology ◽  
2009 ◽  
Vol 385 (1) ◽  
pp. 267-274 ◽  
Author(s):  
Fang Li ◽  
Mingyuan Li ◽  
Wei Ke ◽  
Yongchang Ji ◽  
Xiaofang Bian ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Immediate Early Genes ◽  
Immediate Early ◽  
White Spot ◽  
Early Genes ◽  
White Spot Syndrome
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