scholarly journals Identification of the thymidylate synthase within the genome of white spot syndrome virus

2004 ◽  
Vol 85 (7) ◽  
pp. 2035-2044 ◽  
Author(s):  
Qin Li ◽  
Deng Pan ◽  
Jing-hai Zhang ◽  
Feng Yang
Keyword(s):  
Thymidylate Synthase ◽  
White Spot Syndrome Virus ◽  
De Novo ◽  
Early Gene ◽  
White Spot ◽  
Binding Assays ◽  
Dna Viruses ◽  
Reading Frame ◽  
White Spot Syndrome ◽  
Eukaryotic Organisms

Thymidylate synthase (TS) (EC 2.1.1.45) is essential for the de novo synthesis of dTMP in prokaryotic and eukaryotic organisms. Within the white spot syndrome virus (WSSV) genome, an open reading frame (WSV067) that encodes a 289 amino acid polypeptide showed significant homology to all known TSs from species including mammals, plants, fungi, protozoa, bacteria and DNA viruses. In this study, WSV067 was expressed in Escherichia coli, and the purified recombinant protein showed TS activity in dUMP−folate-binding assays using ultraviolet difference spectroscopy. RT-PCR and Western blot analyses showed that WSV067 was a genuine and early gene. Phylogenetic analysis revealed that WSSV-TS was more closely related to the TSs of eukaryotes than to those from prokaryotes.

scholarly journals Shrimp NF-κB binds to the immediate-early gene ie1 promoter of white spot syndrome virus and upregulates its activity

Virology ◽  
2010 ◽  
Vol 406 (2) ◽  
pp. 176-180 ◽  
Author(s):  
Xian-De Huang ◽  
Li Zhao ◽  
Hai-Qing Zhang ◽  
Xiao-Peng Xu ◽  
Xiao-Ting Jia ◽  
...  
Keyword(s):  
White Spot Syndrome Virus ◽  
Immediate Early Gene ◽  
Early Gene ◽  
Immediate Early ◽  
White Spot ◽  
White Spot Syndrome

scholarly journals Sequencing and De Novo Analysis of the Hemocytes Transcriptome in Litopenaeus vannamei Response to White Spot Syndrome Virus Infection

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e76718 ◽  
Author(s):  
Shuxia Xue ◽  
Yichen Liu ◽  
Yichen Zhang ◽  
Yan Sun ◽  
Xuyun Geng ◽  
...  
Keyword(s):  
Virus Infection ◽  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
De Novo ◽  
White Spot ◽  
White Spot Syndrome

scholarly journals Characterization of an envelope protein (VP110) of White spot syndrome virus

2006 ◽  
Vol 87 (7) ◽  
pp. 1909-1915 ◽  
Author(s):  
Li Li ◽  
Shumei Lin ◽  
Feng Yang
Keyword(s):  
Mass Spectrometry ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Open Reading Frame ◽  
Host Cells ◽  
White Spot ◽  
Genome Database ◽  
Reading Frame ◽  
Rgd Motif ◽  
White Spot Syndrome

A protein of 110 kDa (termed VP110) from the envelope fraction of White spot syndrome virus (WSSV) was identified by SDS-PAGE and mass spectrometry. The resulting amino acid sequence matched an open reading frame (wsv035) containing an Arg–Gly–Asp (RGD) motif in the WSSV genome database. To validate the mass-spectrometry result, the C-terminal segment of the wsv035 open reading frame was expressed in Escherichia coli as a fusion protein, which was used to produce specific antibody. Analysis by Western blotting and immunoelectron microscopy demonstrated that VP110 was an envelope protein of WSSV. An interaction analysis was performed between VP110 and the host cells, using a fluorescence assay and a competitive-inhibition assay. The results showed that VP110 was capable of attaching to host cells and that adhesion could be inhibited by synthetic RGDT peptides, suggesting that the RGD motif in the VP110 sequence may play a role in WSSV infection.

scholarly journals Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus

2002 ◽  
Vol 83 (2) ◽  
pp. 471-477 ◽  
Author(s):  
Xiaobo Zhang ◽  
Canhua Huang ◽  
Xun Xu ◽  
Choy L. Hew
Keyword(s):  
Fusion Protein ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Protein Gene ◽  
Temporal Analysis ◽  
White Spot ◽  
Outer Envelope ◽  
Reading Frame ◽  
Transmission Electron ◽  
White Spot Syndrome

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. In the present study, an open reading frame (termed the p22 gene) was revealed from a WSSV cDNA library. The gene was expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli and purified. Specific antibody was raised using the purified fusion protein (GST–P22). Temporal analysis showed that the p22 gene was a late gene. After binding between purified WSSV virions and anti-GST–P22 IgG followed by labelling with gold-labelled secondary antibody, the gold particles, under a transmission electron microscope, could be found along the outer envelope of WSSV virions. This experiment suggests that the p22 gene encodes an envelope protein of the virus.

scholarly journals A novel envelope protein involved in White spot syndrome virus infection

2005 ◽  
Vol 86 (5) ◽  
pp. 1357-1361 ◽  
Author(s):  
Ru Huang ◽  
Yunli Xie ◽  
Jianhong Zhang ◽  
Zhengli Shi
Keyword(s):  
Escherichia Coli ◽  
Virus Infection ◽  
Envelope Protein ◽  
White Spot Syndrome Virus ◽  
Open Reading Frame ◽  
White Spot ◽  
Structural Protein ◽  
Reading Frame ◽  
Intact Virion ◽  
White Spot Syndrome

One open reading frame (designated vp76) from the White spot syndrome virus (WSSV) genome has the motif of a cytokine I receptor and has been identified as a structural protein. In this paper, vp76 was expressed in Escherichia coli and used to prepare a specific antibody to determine the location of the corresponding protein in the intact virion, the nucleocapsids and the envelope of WSSV. Western blotting with the VP76 antiserum confirmed that VP76 was an envelope protein of WSSV. To investigate the function of the VP76, WSSV was neutralized with the VP76-specific antiserum at different concentrations and injected intramuscularly into crayfish. The mortality curves showed that the VP76 antiserum could partially attenuate infection with WSSV, suggesting that VP76 is an envelope protein involved in WSSV infection.

scholarly journals Characterization of white spot syndrome virus immediate-early gene promoters

2013 ◽  
Vol 94 (2) ◽  
pp. 387-392 ◽  
Author(s):  
Fanyu Lin ◽  
He Huang ◽  
Wei Ke ◽  
Luhong Hou ◽  
Fang Li ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
White Spot Syndrome Virus ◽  
Initiation Site ◽  
Early Gene ◽  
Immediate Early ◽  
White Spot ◽  
Viral Gene ◽  
Gene Promoters ◽  
Promoter Regions ◽  
White Spot Syndrome

Twenty-one immediate-early (IE) genes of white spot syndrome virus (WSSV) have been identified so far. However, the transcriptional regulation of WSSV IE genes remains largely unknown. In this report, the 5′ flanking regions of 18 WSSV IE genes were cloned and eight functional promoter regions were identified. WSSV IE gene promoters normally contained a TATA box approximately 30 bp upstream of the transcriptional initiation site. Also, the cyclic AMP response element (CRE; TGACGTCA) was frequently found within the WSSV IE promoter regions. Mutations of the CREs of WSSV IE promoters P403 and P465 reduced their activity significantly, suggesting that these elements have a role in WSSV IE gene transcription. Our findings provide a more global view of WSSV IE gene promoters and will facilitate the in-depth investigation of viral gene transcriptional regulation.

Sign in / Sign up

Export Citation Format

Share Document