Oestrogen receptor α and β in female rat pituitary cells: An immunochemical study

2008 ◽  
Vol 155 (3) ◽  
pp. 857-868 ◽  
Author(s):  
Miriam González ◽  
Ricardo Reyes ◽  
Carmen Damas ◽  
Rafael Alonso ◽  
Aixa R. Bello
Keyword(s):  
Oestrogen Receptor ◽  
Pituitary Cells ◽  
Female Rat ◽  
Immunochemical Study ◽  
Rat Pituitary ◽  
Oestrogen Receptor Α ◽  
Rat Pituitary Cells

scholarly journals Effects of activin on hormone secretion by single female rat pituitary cells: analysis by cell immunoblot assay

1999 ◽  
Vol 161 (3) ◽  
pp. 375-382 ◽  
Author(s):  
S Miyamoto ◽  
M Irahara ◽  
K Ushigoe ◽  
A Kuwahara ◽  
H Sugino ◽  
...  
Keyword(s):  
Hormone Secretion ◽  
Activin A ◽  
Female Rats ◽  
Pituitary Cells ◽  
Female Rat ◽  
Single Female ◽  
Immunoblot Assay ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Lh Secretion

We investigated the effect of activin A on secretion of LH, FSH, and prolactin (PRL) by female cultured rat pituitary cells at the single-cell level by means of the cell immunoblot assay. Anterior pituitary cells from 8-week-old female rats were preincubated with or without activin A for 24 h, after which they were monodispersed and immediately used for cell immunoblot assay. The percentages of LH-, FSH- and PRL-immunoreactive cell blots detected were 5.5, 5.3 and 43.1%, respectively, of all pituitary cells applied to the transfer membrane. The percentage of LH-secreting cells and mean LH secretion per cell did not change after treatment with activin. In contrast, activin significantly increased the percentage of FSH-secreting cells and mean FSH secretion per cell to 136.0 and 114. 5% respectively. In addition, activin significantly decreased the percentage of PRL-secreting cells and mean PRL secretion per cell to 52.2 and 72.0% respectively. These results suggest that (1) activin A has effects on female rat pituitary cells that increase not only the amount of FSH secretion per cell but also the number of FSH-secreting cells, and (2) activin A decreases both the amount of PRL secretion per cell and the number of PRL-secreting cells.

Neuropeptide glutamic-isoleucine (NEI) specifically stimulates the secretory activity of gonadotrophs in primary cultures of female rat pituitary cells

Peptides ◽  
2009 ◽  
Vol 30 (11) ◽  
pp. 2081-2087 ◽  
Author(s):  
Ana Lucía De Paul ◽  
Andrés Maximiliano Attademo ◽  
Ruben Walter Carón ◽  
Marta Soaje ◽  
Alicia Inés Torres ◽  
...  
Keyword(s):  
Primary Cultures ◽  
Secretory Activity ◽  
Pituitary Cells ◽  
Female Rat ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

SPONTANEOUS RELEASE OF IMMUNOREACTIVE ACTH FROM ISOLATED RAT PITUITARY CELLS

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S162
Author(s):  
H. L. Fehm ◽  
K. H. Voigt ◽  
R. Lang ◽  
M. Schleyer ◽  
E. F. Pfeiffer
Keyword(s):  
Pituitary Cells ◽  
Spontaneous Release ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Isolated Rat

Modulation of prolactin secretion by contraceptive progestins in cultured rat pituitary cells in vitro

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S188-S189
Author(s):  
L. KIESEL ◽  
T. RABE ◽  
D. SCHOLZ ◽  
V. KIRSCHNER ◽  
B. RUNNEBAUM
Keyword(s):  
Prolactin Secretion ◽  
Pituitary Cells ◽  
Rat Pituitary ◽  
Rat Pituitary Cells
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