scholarly journals A New Method to Extract Nuclei from Paraffin-Embedded Tissue to Study Lymphomas Using Interphase Fluorescence in Situ Hybridization

2002 ◽  
Vol 160 (6) ◽  
pp. 1967-1972 ◽  
Author(s):  
Sarah F. Paternoster ◽  
Stephanie R. Brockman ◽  
Rebecca F. McClure ◽  
Ellen D. Remstein ◽  
Paul J. Kurtin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
New Method

Fluorescence in situ hybridization: a new method for determining primary sex ratio in ants

2003 ◽  
Vol 12 (6) ◽  
pp. 1637-1648 ◽  
Author(s):  
L. De Menten ◽  
H. Niculita ◽  
M. Gilbert ◽  
D. Delneste ◽  
S. Aron
Keyword(s):  
In Situ Hybridization ◽  
Sex Ratio ◽  
Fluorescence In Situ Hybridization ◽  
New Method ◽  
Primary Sex Ratio

scholarly journals Increased Efficiency of Fluorescence In Situ Hybridization (FISH) Using the Microwave

2005 ◽  
Vol 53 (10) ◽  
pp. 1301-1303 ◽  
Author(s):  
Anja Weise ◽  
Thomas Liehr ◽  
Uwe Claussen ◽  
Karl-Jürgen Halbhuber
Keyword(s):  
In Situ Hybridization ◽  
Microwave Irradiation ◽  
Fluorescence In Situ Hybridization ◽  
Dna Hybridization ◽  
New Method ◽  
Weak Signals ◽  
Microwave Pulses ◽  
Normal Fish

A new method is described for performing fluorescence in situ hybridization (FISH). FISH signals are enhanced by microwave pulses applied during the DNA-DNA hybridization process. It is the first description of FISH with a single/low-copy probe done more efficiently by application of microwave; the latter leads to quick results or enhancement of weak signals. Microwave FISH has been compared systematically with normal FISH, and we could demonstrate the efficiency of microwave irradiation especially in the first 100 min of hybridization.

890: Multiple Fluorescence in Situ Hybridization (FISH) Probes Increase Sensitivity for Detection of Bladder Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 287-288 ◽  
Author(s):  
Juliann M. Dziubinski ◽  
Michael F. Sarosdy ◽  
Paul R. Kahn ◽  
Mark D. Ziffer ◽  
William R. Love ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization

588: Detection of Chromosome 8 Alterations in Paired Needle Biopsy and Prostatectomy Specimens Using Fluorescence In-Situ Hybridization

2004 ◽  
Vol 171 (4S) ◽  
pp. 156-156
Author(s):  
Chandler D. Dora ◽  
Yasushi Kondo ◽  
Fusheng X. Lan ◽  
Jeffrey M. Slezak ◽  
Erik J. Bergstralh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Needle Biopsy ◽  
Chromosome 8

A PILOT STUDY OF HER-2/NEU GENE AMPLIFICATION ASSESSED BY FLUORESCENCE IN SITU HYBRIDIZATION (FISH) IN GASTRIC CANCER

2014 ◽  
pp. 15-20
Author(s):  
Van Huy Tran ◽  
Thi Minh Thi Ha ◽  
Trung Nghia Van ◽  
Viet Nhan Nguyen ◽  
Phan Tuong Quynh Le ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cancer Patients ◽  
Gene Amplification ◽  
Cancer Tissue ◽  
Tissue Samples ◽  
Her 2 ◽  
Gastric Cancer Patients

Background: HER-2/neu is a predictive biomarker for treatment of gastric cancer using trastuzumab in combination with chemotherapy. This study aimed to evaluate the status of HER-2/neu gene amplification using fluorescence in situ hybridization (FISH) in gastric cancer. Patients and methods: thirty six gastric cancer patients were assessed HER-2/neu gene amplification by FISH using PathVysionTM HER-2 DNA Probe kit (including HER-2/neu probe and CEP-17 probe) with biopsy and surgical specimens. Results: The HER-2/neu gene amplification was observed in three cases (8.3%), the HER-2/neu gene amplification rate in Lauren’s intestinal-type and diffuse-type were 11.8% and 5.2%, respectively. Conclusion: We applied successfully FISH technique with gastric cancer tissue samples. This technique could be performed as routine test in gastric cancer in order to select patients that benefit from trastuzumab in combination with chemotherapy.

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