An enzymatic method for the quantitative determination of 3-keto bile acids

1979 ◽  
Vol 99 (2) ◽  
pp. 408-414 ◽  
Author(s):  
M.S. Anwer ◽  
D. Hegner
1972 ◽  
Vol 39 (2) ◽  
pp. 524-531 ◽  
Author(s):  
Andrée M. Weber ◽  
Liette Chartrand ◽  
Gilles Doyon ◽  
Stuart Gordon ◽  
Claude C. Roy

1968 ◽  
Vol 22 ◽  
pp. 1729-1744 ◽  
Author(s):  
Peter Eneroth ◽  
Kjell Hellström ◽  
Jan Sjövall ◽  
N. A. Zaidi

Author(s):  
Per Tobiasson ◽  
Magnus Källberg

An enzymatic method (Sterognost-3α® Flu, Nyegaard & Co, Oslo, Norway) for the determination of total serum bile acids has been evaluated, and reference values for fasting and postprandial total serum bile acids have been established. Precision was rather low in the lower normal range but satisfactory in the upper reference range and for elevated values. The upper reference value for fasting total serum bile acids was 5·0 μmol/l. Maximal postprandial elevations occurred at varying intervals after a test meal and the upper postprandial reference value was 12 μmol/l. Certain serum samples were also analysed by radioimmunological methods for conjugates of cholic and chenodeoxycholic acids. The values obtained with the enzymatic method correlated satisfactorily with those of the radioimmunoassay methods for most of the serum samples analysed. However, a number of samples with slightly elevated bile acid concentrations, as measured by the comparison radioimmunoassay methods, were found to have normal total serum bile acids when measured by the enzymatic method.


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