The main immunogenic region of the nicotinic acetylcholine receptor: Interaction of monoclonal antibodies with synthetic peptides

1989 ◽  
Vol 165 (2) ◽  
pp. 865-871 ◽  
Author(s):  
Manoj K. Das ◽  
Jon Lindstrom
Keyword(s):  
Monoclonal Antibodies ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Synthetic Peptides ◽  
Receptor Interaction ◽  
Nicotinic Acetylcholine ◽  
Immunogenic Region

scholarly journals Characterization of α-conotoxin interactions with the nicotinic acetylcholine receptor and monoclonal antibodies

1997 ◽  
Vol 328 (1) ◽  
pp. 245-250 ◽  
Author(s):  
D. James ASHCOM ◽  
G. Bradley STILES
Keyword(s):  
Monoclonal Antibodies ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Dissociation Constants ◽  
Biologically Active ◽  
Binding Assays ◽  
Nicotinic Acetylcholine ◽  
Disulphide Bonds ◽  
Conus Species

The venoms of predatory marine cone snails, Conus species, contain numerous peptides and proteins with remarkably diverse pharmacological properties. One group of peptides are the α-conotoxins, which consist of 13-19 amino acids constrained by two disulphide bonds. A biologically active fluorescein derivative of Conus geographus α-conotoxin GI (FGI) was used in novel solution-phase-binding assays with purified Torpedo californica nicotinic acetylcholine receptor (nAchR) and monoclonal antibodies developed against the toxin. The binding of FGI to nAchR or antibody had apparent dissociation constants of 10-100 nM. Structure-function studies with α-conotoxin GI analogues composed of a single disulphide loop revealed that different conformational restraints are necessary for effective toxin interactions with nAchR or antibodies.

scholarly journals Peptide Inhibitors of the α-Cobratoxin–Nicotinic Acetylcholine Receptor Interaction

2020 ◽  
Vol 63 (22) ◽  
pp. 13709-13718
Author(s):  
Timothy Lynagh ◽  
Stephan Kiontke ◽  
Maria Meyhoff-Madsen ◽  
Bengt H. Gless ◽  
Jónas Johannesen ◽  
...  
Keyword(s):  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Peptide Inhibitors ◽  
Receptor Interaction ◽  
Nicotinic Acetylcholine

Interaction of nicotinic acetylcholine receptor with two monoclonal antibodies recognizing different epitopes

Biochemistry ◽  
1989 ◽  
Vol 28 (10) ◽  
pp. 4222-4229 ◽  
Author(s):  
Miguel A. Chinchetru ◽  
Javier Marquez ◽  
Jose C. Garcia-Borron ◽  
David P. Richman ◽  
Marino Martinez-Carrion
Keyword(s):  
Monoclonal Antibodies ◽  
Acetylcholine Receptor ◽  
Nicotinic Acetylcholine Receptor ◽  
Nicotinic Acetylcholine
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