scholarly journals Patch electrode glass composition affects ion channel currents

1988 ◽  
Vol 53 (2) ◽  
pp. 287-292 ◽  
Author(s):  
R.E. Furman ◽  
J.C. Tanaka
Keyword(s):  
Ion Channel ◽  
Glass Composition ◽  
Channel Currents ◽  
Electrode Glass

scholarly journals Inhibition of Acid Sensing Ion Channel Currents by Lidocaine in Cultured Mouse Cortical Neurons

2011 ◽  
Vol 112 (4) ◽  
pp. 977-981 ◽  
Author(s):  
Jun Lin ◽  
Xiangping Chu ◽  
Samaneh Maysami ◽  
Minghua Li ◽  
Hongfang Si ◽  
...  
Keyword(s):  
Ion Channel ◽  
Cortical Neurons ◽  
Channel Currents

scholarly journals Development of an automated system to measure ion channel currents using a surface-modified gold probe

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Minako Hirano ◽  
Masahisa Tomita ◽  
Chikako Takahashi ◽  
Nobuyuki Kawashima ◽  
Toru Ide
Keyword(s):  
Ion Channels ◽  
Ion Channel ◽  
Lipid Bilayer ◽  
Measurement System ◽  
Automated System ◽  
Channel Activity ◽  
Channel Current ◽  
Gold Probe ◽  
Measurement Efficiency ◽  
Channel Currents

AbstractArtificial lipid bilayer single-channel recording technique has been employed to determine the biophysical and pharmacological properties of various ion channels. However, its measurement efficiency is very low, as it requires two time-consuming processes: preparation of lipid bilayer membranes and incorporation of ion channels into the membranes. In order to address these problems, we previously developed a technique based on hydrophilically modified gold probes on which are immobilized ion channels that can be promptly incorporated into the bilayer membrane at the same time as the membrane is formed on the probes’ hydrophilic area. Here, we improved further this technique by optimizing the gold probe and developed an automated channel current measurement system. We found that use of probes with rounded tips enhanced the efficiency of channel current measurements, and introducing a hydrophobic area on the probe surface, beside the hydrophilic one, further increased measurement efficiency by boosting membrane stability. Moreover, we developed an automated measurement system using the optimized probes; it enabled us to automatically measure channel currents and analyze the effects of a blocker on channel activity. Our study will contribute to the development of high-throughput devices to identify drug candidates affecting ion channel activity.

scholarly journals Modulation of Acid-sensing Ion Channel Currents, Acid-induced Increase of Intracellular Ca2+, and Acidosis-mediated Neuronal Injury by Intracellular pH

2006 ◽  
Vol 281 (39) ◽  
pp. 29369-29378 ◽  
Author(s):  
Wei-Zhen Wang ◽  
Xiang-Ping Chu ◽  
Ming-Hua Li ◽  
Joshua Seeds ◽  
Roger P. Simon ◽  
...  
Keyword(s):  
Ion Channel ◽  
Intracellular Ph ◽  
Neuronal Injury ◽  
Channel Currents

Cation and anion unitary ion channel currents in cultured bovine microglia

1995 ◽  
Vol 693 (1-2) ◽  
pp. 8-20 ◽  
Author(s):  
James G. McLarnon ◽  
Dale Sawyer ◽  
Seung U. Kim
Keyword(s):  
Ion Channel ◽  
Channel Currents

Ion-channel currents of smooth muscle cells isolated from the prostate of guinea-pig

2003 ◽  
Vol 92 (9) ◽  
pp. 1022-1030 ◽  
Author(s):  
S.-J. Oh ◽  
K.M. Kim ◽  
Y.-S. Chung ◽  
E.-K. Hong ◽  
S.Y. Shin ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Ion Channel ◽  
Muscle Cells ◽  
Channel Currents

Effects of estradiol on cardiac ion channel currents

2006 ◽  
Vol 532 (1-2) ◽  
pp. 44-49 ◽  
Author(s):  
Clemens Möller ◽  
Rainer Netzer
Keyword(s):  
Ion Channel ◽  
Channel Currents
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