A Herd Test for Non-Pregnancy Using Plasma Progesterone Levels in the Selection of Swamp Buffalo for Oestrus Synchronization

1981 ◽  
Vol 137 (2) ◽  
pp. 173-175 ◽  
Author(s):  
M. Kamonpatana ◽  
P. Chantaraprateep ◽  
C. Ngramsuriyaroj
Keyword(s):  
Plasma Progesterone ◽  
Swamp Buffalo ◽  
Selection Of

266 BIRTH OF CANINE OFFSPRING FOLLOWING INSEMINATION OF A BITCH WITH FLOW-SORTED SPERMATOZOA

2008 ◽  
Vol 20 (1) ◽  
pp. 213 ◽  
Author(s):  
M. A. Meyers ◽  
G. Burns ◽  
D. Arn ◽  
J. L. Schenk
Keyword(s):  
X Chromosome ◽  
Dna Content ◽  
Egg Yolk ◽  
Labrador Retriever ◽  
Plasma Progesterone ◽  
Sheath Fluid ◽  
Natural Birth ◽  
Fort Collins ◽  
Respective Number ◽  
Selection Of

Different traits and temperaments that are sex-related often underlie the selection of guide dogs used for auditory and/or visually impaired humans. The objective of this project was to select X-chromosome-bearing canine sperm by means of flow cytometry/cell sorting based on DNA content (X chromosome- bearing canine sperm contain 3.7% more DNA than Y-chromosome-bearing sperm) and use the sorted sperm for artificial insemination (AI) to produce live offspring. This technology has proven reliable for sex selecting offspring using AI in a number of species and is of great commercial value to food animal livestock production. To test the efficacy of sorted canine sperm, a 2-year-old nulliparous Labrador retriever bitch in observed heat was monitored for plasma progesterone levels for a baseline rise to 2.8 ng mL–1, indicative of the LH surge. Progesterone levels more than doubled (5.8 ng mL–1) 2 days later, suggesting ovulation. One ejaculate was collected from a proven Labrador retriever stud on each of Days 2, 5, and 6 following the estimated LH peak. Sperm (200 million) were diluted in TRIS buffer without egg yolk (pH 7.4) and stained with 97.4 µm Hoechst 33342 in 1-mL aliquots for 45 min in a 34.5�C water bath and then diluted to 100 million sperm mL–1 with 4% egg yolk TRIS (pH 6.2) containing 0.002% food coloring dye (FD&C #40). Sperm were sex-sorted using an SX MoFlo™ sperm sorter (Dako, Fort Collins, CO, USA), operated at 40 psi and using a TRIS-based medium (pH = 6.8, 370 mOsm kg–1 water) as sheath fluid: 10 000–20 000 events s–1 and sort rates of 800–2000 sperm s–1. Sorted sperm were concentrated by centrifugation for 20 min at 850g and, after the supernatant was removed, were extended with 20% egg yolk TRIS extender (pH 6.8) to a total volume of 2 mL. Transcervical insemination of fresh, sorted sperm occurred within 9 h of the semen collection. An additional 1 mL of 20% egg yolk TRIS extender was used to flush the insemination catheter. The respective number of X-chromosome-bearing sperm inseminated per day was 46.2, 20.3, and 18.2 million, totaling 84.7 million sperm, and the weighted purity mean across the 3 sort days was 82%. Pregnancy was ascertained by ultrasound 30 days post-AI. Five puppies (3 female and 2 male) were delivered via natural birth 62 days after first AI. Only 60% of the offspring were of the predicted sex due to an 18% probability of chance, given the weighted mean purity of the sorted sperm and for a litter of 5 puppies. This demonstrates that fresh canine sperm can be separated by sex on the basis of DNA content, and transcervical insemination of low numbers of non-frozen sorted canine sperm can be used to produce live offspring. This work was funded by XY, Inc.

Plasma progesterone profiles in Beagle bitches with and without the whelping experience

2010 ◽  
Vol 58 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Makoto Seki ◽  
Norio Watanabe ◽  
Kenyo Ishii ◽  
Yoh-ichi Kinoshita ◽  
Takehiro Aihara ◽  
...  
Keyword(s):  
Plasma Progesterone ◽  
The Mean ◽  
Initial Elevation ◽  
Selection Of

The aim of this study was to investigate differences between the progesterone profiles of Beagle bitches with (multiparous) and without (nulliparous) the whelping experience and to examine whether the selection of bitches by progesterone analyses before the programmed mating improved the whelping rates. In the first experiment, the progesterone profiles of nulliparous and multiparous bitches were evaluated from Days 1 to 13 (onset of prooestrus = Day 0). The mean duration of the elevation in progesterone levels (> 2 ng/mL) after the onset of prooestrus tended to be approximately 1 day shorter in nulliparous bitches (7.7 days) than in multiparous bitches (8.5 days). In the second experiment, progesterone analyses in the bitches were carried out once on Days 4, 5 or 6. Bitches with progesterone levels of > 10 ng/mL were excluded from mating because it was unclear when the progesterone levels reached > 10 ng/mL considering the optimal date for mating. No significant differences were observed in the percentages of bitches excluded from mating and in the whelping rates of the mated bitches between the groups, irrespective of the day of progesterone analysis and the type of bitch. In conclusion, the initial elevation of progesterone levels was of shorter duration in nulliparous bitches. The selection of bitches by the measurement of progesterone levels once before mating was not effective for the programmed mating.

OESTRUS CONTROL AND EARLY PREGNANCY DIAGNOSIS IN THE SWAMP BUFFALO: COMPARISON OF ENZYME IMMUNOASSAY AND RADIOIMMUNOASSAY FOR PLASMA PROGESTERONE

1981 ◽  
pp. 141-147
Author(s):  
Maneewan Kamonpatana ◽  
Dick F.M. van de Wiel ◽  
Wim Koops ◽  
Dumrong Leenanuruksa ◽  
Chunpen Ngramsuriyaroj ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Early Pregnancy ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis ◽  
Swamp Buffalo

scholarly journals Selection of cow buffalo and its effect on reproductive characteristic of swamp buffalo

2020 ◽  
Vol 465 ◽  
pp. 012043
Author(s):  
La Ode Nafiu ◽  
Takdir Saili ◽  
Ali Bain ◽  
Firman Nasiu ◽  
Musram Abadi ◽  
...  
Keyword(s):  
Reproductive Characteristic ◽  
Swamp Buffalo ◽  
Selection Of

Oestrus control and early pregnancy diagnosis in the swamp buffalo: Comparison of enzymeimmunoassay and radioummunoassay for plasma progesterone

1979 ◽  
Vol 11 (5) ◽  
pp. 399-409 ◽  
Author(s):  
Maneewan Kamonpatana ◽  
Dick F.M. van de Wiel ◽  
Wim Koops ◽  
Dumrong Leenanuruksa ◽  
Chunpen Ngramsuriyaroj ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Plasma Progesterone ◽  
Pregnancy Diagnosis ◽  
Swamp Buffalo

Cognitive gadgets and cognitive priors

2019 ◽  
Vol 42 ◽  
Author(s):  
Gian Domenico Iannetti ◽  
Giorgio Vallortigara
Keyword(s):  
Cognitive Neuroscience ◽  
Selection Of

Abstract Some of the foundations of Heyes’ radical reasoning seem to be based on a fractional selection of available evidence. Using an ethological perspective, we argue against Heyes’ rapid dismissal of innate cognitive instincts. Heyes’ use of fMRI studies of literacy to claim that culture assembles pieces of mental technology seems an example of incorrect reverse inferences and overlap theories pervasive in cognitive neuroscience.

Note on Selection of Coordinate Systems for Geodynamics

1975 ◽  
Vol 26 ◽  
pp. 395-407
Author(s):  
S. Henriksen
Keyword(s):  
Sea Level ◽  
The Other ◽  
Coordinate Systems ◽  
Mean Sea Level ◽  
The Earth ◽  
The One ◽  
Static Systems ◽  
Selection Of

The first question to be answered, in seeking coordinate systems for geodynamics, is: what is geodynamics? The answer is, of course, that geodynamics is that part of geophysics which is concerned with movements of the Earth, as opposed to geostatics which is the physics of the stationary Earth. But as far as we know, there is no stationary Earth – epur sic monere. So geodynamics is actually coextensive with geophysics, and coordinate systems suitable for the one should be suitable for the other. At the present time, there are not many coordinate systems, if any, that can be identified with a static Earth. Certainly the only coordinate of aeronomic (atmospheric) interest is the height, and this is usually either as geodynamic height or as pressure. In oceanology, the most important coordinate is depth, and this, like heights in the atmosphere, is expressed as metric depth from mean sea level, as geodynamic depth, or as pressure. Only for the earth do we find “static” systems in use, ana even here there is real question as to whether the systems are dynamic or static. So it would seem that our answer to the question, of what kind, of coordinate systems are we seeking, must be that we are looking for the same systems as are used in geophysics, and these systems are dynamic in nature already – that is, their definition involvestime.

Measurements of the Cape Astrometric Survey of the Southern Hemisphere

1978 ◽  
Vol 48 ◽  
pp. 515-521
Author(s):  
W. Nicholson
Keyword(s):  
Southern Hemisphere ◽  
Automatic Measuring ◽  
Measuring Machine ◽  
Final Selection ◽  
Selection Of

SummaryA routine has been developed for the processing of the 5820 plates of the survey. The plates are measured on the automatic measuring machine, GALAXY, and the measures are subsequently processed by computer, to edit and then refer them to the SAO catalogue. A start has been made on measuring the plates, but the final selection of stars to be made is still a matter for discussion.

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