Purification and biochemical characterization of xanthopterin from patients with chronic renal failure. II. Biochemical elucidation and structural analysis

This paper presents a study of the families of children on peritoneal dialysis, emphasizing the identification of social supports and networks to strengthen interventions aimed at health promotion. Our discussion is located in the context of inequalities between developed and developing countries. For this qualitative study, a content analysis was conducted in order to elicit themes from the raw data related to the lived experience of four families that have a child with chronic renal failure. The data were collected mainly by in-depth interviews and the construction of genograms and ecomaps. The identification and characterization of the families' social supports and networks allowed nurses and families to strengthen their coping mechanisms. Because families are dealing with severe economic problems, they need better supportive programs to guide their offspring to their full potential.

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Functional characterization of cytokine autoantibodies in chronic renal failure patients

Kidney International ◽

10.1038/ki.1994.193 ◽

1994 ◽

Vol 45 (5) ◽

pp. 1484-1488 ◽

Cited By ~ 11

Author(s):

Gere Sunder-Plassmann ◽

Stelio Kapiotis ◽

Christoph Gasche ◽

Ursula Klaar ◽

Brigitte Mai

Keyword(s):

Renal Failure ◽

Chronic Renal Failure ◽

Functional Characterization

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Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism

10.1101/2020.05.24.113720 ◽

2020 ◽

Cited By ~ 1

Author(s):

Robert J. Nichols ◽

Benjamin LaFrance ◽

Naiya R. Phillips ◽

Luke M. Oltrogge ◽

Luis E. Valentin-Alvarado ◽

...

Keyword(s):

Structural Analysis ◽

Biochemical Characterization ◽

Sulfur Metabolism ◽

Physiological Role ◽

Enzymatic Characterization ◽

Cysteine Desulfurase ◽

Future Studies ◽

Pcc 7942

AbstractProkaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of encapsulins, bioinformatic evidence suggests that a great diversity of encapsulin nanocompartments remains unexplored. Here, we describe a novel encapsulin in the freshwater cyanobacterium Synechococcus elongatus PCC 7942. This nanocompartment is upregulated upon sulfate starvation and encapsulates a cysteine desulfurase enzyme via an N-terminal targeting sequence. Using cryoelectron microscopy, we have determined the structure of the nanocompartment complex to 2.2 Å resolution. Lastly, biochemical characterization of the complex demonstrated that the activity of the cysteine desulfurase is enhanced upon encapsulation. Taken together, our discovery, structural analysis, and enzymatic characterization of this prokaryotic nanocompartment provide a foundation for future studies seeking to understand the physiological role of this encapsulin in various bacteria.

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