Specific defects in double-stranded DNA unwinding and homologous pairing of a mutant RecA protein

FEBS Letters ◽  
2000 ◽  
Vol 477 (1-2) ◽  
pp. 129-134 ◽  
Author(s):  
Hitoshi Kurumizaka ◽  
Hideki Aihara ◽  
Shukuko Ikawa ◽  
Takehiko Shibata
Keyword(s):  
Reca Protein ◽  
Dna Unwinding ◽  
Homologous Pairing ◽  
Double Stranded Dna

An interaction between a specified surface of the C-terminal domain of RecA protein and double-stranded DNA for homologous pairing

1997 ◽  
Vol 274 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Hideki Aihara ◽  
Yutaka Ito ◽  
Hitoshi Kurumizaka ◽  
Tohru Terada ◽  
Shigeyuki Yokoyama ◽  
...  
Keyword(s):  
Reca Protein ◽  
Homologous Pairing ◽  
Terminal Domain ◽  
Double Stranded Dna

scholarly journals RecA Protein Promoted Homologous Pairing in Vitro

1989 ◽  
Vol 264 (29) ◽  
pp. 17395-17400 ◽  
Author(s):  
J Ramdas ◽  
E Mythili ◽  
K Muniyappa
Keyword(s):  
Reca Protein ◽  
Homologous Pairing

scholarly journals The search for DNA homology does not limit stable homologous pairing promoted by RecA protein

1995 ◽  
Vol 5 (10) ◽  
pp. 1149-1158 ◽  
Author(s):  
Janet E. Yancey-Wrona ◽  
R.Daniel Camerini-Otero
Keyword(s):  
Reca Protein ◽  
Dna Homology ◽  
Homologous Pairing

scholarly journals Mechanisms of fast and stringent search in homologous pairing of double-stranded DNA

2017 ◽  
Vol 13 (3) ◽  
pp. e1005421 ◽  
Author(s):  
Amir Bitran ◽  
Wei-Yin Chiang ◽  
Erel Levine ◽  
Mara Prentiss
Keyword(s):  
Homologous Pairing ◽  
Double Stranded Dna

scholarly journals Effects of recJ, recQ, and recFOR Mutations on Recombination in Nuclease-Deficient recB recD Double Mutants of Escherichia coli

2005 ◽  
Vol 187 (4) ◽  
pp. 1350-1356 ◽  
Author(s):  
Ivana Ivančić-Baće ◽  
Erika Salaj-Šmic ◽  
Krunoslav Brčić-Kostić
Keyword(s):  
Escherichia Coli ◽  
Double Mutant ◽  
Reca Protein ◽  
Recq Helicase ◽  
Single Stranded Dna ◽  
Double Stranded Dna ◽  
Recf Pathway ◽  
Recombination Pathway ◽  
Recbcd Enzyme ◽  
Dna Ends

ABSTRACT The two main recombination pathways in Escherichia coli (RecBCD and RecF) have different recombination machineries that act independently in the initiation of recombination. Three essential enzymatic activities are required for early recombinational processing of double-stranded DNA ends and breaks: a helicase, a 5′→3′ exonuclease, and loading of RecA protein onto single-stranded DNA tails. The RecBCD enzyme performs all of these activities, whereas the recombination machinery of the RecF pathway consists of RecQ (helicase), RecJ (5′→3′ exonuclease), and RecFOR (RecA-single-stranded DNA filament formation). The recombination pathway operating in recB (nuclease-deficient) mutants is a hybrid because it includes elements of both the RecBCD and RecF recombination machineries. In this study, genetic analysis of recombination in a recB (nuclease-deficient) recD double mutant was performed. We show that conjugational recombination and DNA repair after UV and gamma irradiation in this mutant are highly dependent on recJ, partially dependent on recFOR, and independent of recQ. These results suggest that the recombination pathway operating in a nuclease-deficient recB recD double mutant is also a hybrid. We propose that the helicase and RecA loading activities belong to the RecBCD recombination machinery, while the RecJ-mediated 5′→3′ exonuclease is an element of the RecF recombination machinery.

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