Abstract
Human embryogenesis is not well understood. Knowledge detailing human pre-gastrulation embryonic development including spatial self-organization and cell type ontogeny remains limited by available two-dimensional technological platforms. Here, we present a three-dimensional (3D) blastocyst-culture system, which enables human blastocyst development through primitive streak anlage (PSA). By the 3D-platform combined with immunofluorescence imaging and single-cell RNA-Seq, we reveal a developmental landscape of human pre-gastrulation embryos. Our protocol allows recording and analysis of embryo developmental landmarks and mechanisms from human blastocysts to pre-gastrulation stage (day 14 post- fertilization).
Effect of Growth Supplementation on Blastocyst Development in Sequential Human Blastocyst Culture Medium