Effects of human oviductal cell coculture on various functional parameters of human spermatozoa

1999 ◽  
Vol 71 (2) ◽  
pp. 232-239 ◽  
Author(s):  
Yuan-qing Yao ◽  
Pak-chung Ho ◽  
William Shu-biu Yeung
Keyword(s):  
Human Spermatozoa ◽  
Functional Parameters ◽  
Oviductal Cell

Effects of long-term in vitro incubation of human spermatozoa: functional parameters and catalase effect

Andrologia ◽  
2001 ◽  
Vol 33 (2) ◽  
pp. 79-86 ◽  
Author(s):  
J. C. Calamera ◽  
P. J. Fernandez ◽  
M. G. Buffone ◽  
A. A. Acosta ◽  
G. F. Doncel
Keyword(s):  
Human Spermatozoa ◽  
Functional Parameters ◽  
In Vitro Incubation

scholarly journals Quantification of CatSper1 expression in human spermatozoa and relation to functional parameters

2015 ◽  
Vol 30 (7) ◽  
pp. 1532-1544 ◽  
Author(s):  
L. Tamburrino ◽  
S. Marchiani ◽  
E. Vicini ◽  
B. Muciaccia ◽  
M. Cambi ◽  
...  
Keyword(s):  
Human Spermatozoa ◽  
Functional Parameters

The Fine Structure of Glycocalyx in Human Spermatozoa. A High Resolution Cytochemical Study

1973 ◽  
Vol 31 ◽  
pp. 640-641
Author(s):  
P. Hernández-Jáuregui ◽  
A. Sosa ◽  
A. González Angulo
Keyword(s):  
Negative Charge ◽  
Iron Hydroxide ◽  
Human Spermatozoa ◽  
Surface Coat ◽  
Cell Surfaces ◽  
Colloidal Iron ◽  
Cytochemical Study ◽  
Specific Permeability ◽  
Extracellular Glycoprotein ◽  
Mammalian Spermatozoa

Glycocalyx is the name given by Bennett to the extracellular glycoprotein coat present in some cell surfaces. It appears to play an important role in cell properties such as antigenicity, cell adhesivity, specific permeability, and ATP ase activity. In the sperm this coat can be directly related to such important phenomena as capacitation and fertilization. The presence of glycocalyx in invertebrate spermatozoa has already been demonstrated. Recently Yanagimachi et al. has determined the negative charges on sperm surfaces of mammalian spermatozoa including man, using colloidal iron hydroxide. No mention was made however of the outer surface coat as composed of substances other than those confering a negative charge. The purpose of this work was therefore to determine the presence of a glycocalyx in human spermatozoa using alcian blue and lanthanum staining.

A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

1975 ◽  
Vol 33 ◽  
pp. 594-595
Author(s):  
A. Sosa ◽  
L. Calzada
Keyword(s):  
High Resolution ◽  
Atpase Activity ◽  
Nuclear Membrane ◽  
Human Sperm ◽  
Sperm Nucleus ◽  
Human Spermatozoa ◽  
Cytochemical Study ◽  
Membrane Bound ◽  
Sperm Nuclei ◽  
Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

Membrane fluidity and lipid content of human spermatozoa selected by swim-up method

2001 ◽  
Vol 24 (6) ◽  
pp. 327-334 ◽  
Author(s):  
A. Force ◽  
G. Grizard ◽  
M. N. Giraud ◽  
C. Motta ◽  
B. Sion ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Lipid Content ◽  
Human Spermatozoa

Response to DDAVP in children with von Willebrand disease type 2

2009 ◽  
Vol 29 (02) ◽  
pp. 143-148 ◽  
Author(s):  
U. Budde ◽  
K. Beutel ◽  
W.-A. Hassenpflug ◽  
H. Hauch ◽  
T. Obser ◽  
...  
Keyword(s):  
Disease Type ◽  
Von Willebrand Disease ◽  
Molecular Defect ◽  
Variable Response ◽  
Functional Parameters ◽  
Functional Defect ◽  
Biologic Response ◽  
High Level ◽  
Von Willebrand

SummaryWe have prospectively evaluated the biologic response to desmopressin (DDAVP) in 28 children with type 2 von Willebrand disease (VWD) in correlation with the phenotype and the molecular defect of VWF. The diagnosis of VWD type 2 was mainly based on VWF functional parameters and/or an aberrant VWF multimer pattern. Seventeen different mutations were identified (6 of them novel). No response with respect to the functional parameters VWF:RCo and/or VWF:CB was seen in patients with severe abnormality of the VWF multimer pattern. One patient with VWD type 2A phenotype IIC Miami did not respond with respect to VWF:CB, but showed a good response of VWF:Ag and FVIII:C as expected. Interestingly he showed a persistently high level of VWF:Ag and FVIII:C up to 4 hours after DDAVP infusion. Patients with minor alterations of multimer structure and particular mutations responded well to DDAVP, whereas patients with normal multimer structure but a defect in platelet dependent functional parameters did not respond with VWF:RCo. Conclusion: Children with VWD type 2 show a variable response to desmopressin depending on the mutation that correlates with the functional defect and the presence or absence as well as the half-life of large VWF multimers. Our data emphasize the usefulness of DDAVP testing even in patients with VWD type 2, possibly with the exception of VWD type 2B.

IMPORTANT FUNCTIONAL PARAMETERS OF SPERMATOZOA: A REVIEW

2018 ◽  
Vol 7 (4) ◽  
pp. 98-111
Author(s):  
KUMAR AMIT ◽  
GANGULY SUBHA ◽  
PAGRUT NILESHKUMAR
Keyword(s):  
Functional Parameters
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