Removal of Excess Alkali from Sodium Naphthenate Solution by Electrodialysis Using Bilayer Membranes for Subsequent Conversion to Naphthenic Acids

The processing of solutions containing sodium salts of naphthenic acids (sodium naphthenate) is in high demand due to the high value of the latter. Such solutions usually include an excessive amount of alkali and a pH of around 13. Bipolar electrodialysis can convert sodium naphthenates into naphthenic acids; however, until pH 6.5, the naphthenic acids are not released from the solution. The primary process leading to a decrease in pH is the removal of excess alkali that implies that some part of electricity is wasted. In this work, we propose a technique for the surface modification of anion-exchange membranes with sulfonated polyetheretherketone, with the formation of bilayer membranes that are resistant to poisoning by the naphthenate anions. We investigated the electrochemical properties of the obtained membranes and their efficiency in a laboratory electrodialyzer. Modified membranes have better electrical conductivity, a high current efficiency for hydroxyl ions, and a low tendency to poisoning than the commercial membrane MA-41. We propose that the primary current carrier is the hydroxyl ion in both electromembrane systems with the MA-41 and MA-41M membranes. At the same time, for the modified MA-41M membrane, the concentration of hydroxyl ions in the anion-exchanger phase is higher than in the MA-41 membrane, which leads to almost five-fold higher values of the specific permeability coefficient. The MA-41M membranes are resistant to poisoning by naphthenic acids anions during at least six cycles of processing of the sodium naphthenate solution.

Mathematical Modeling of the Effect of Pulsed Electric Field on the Specific Permselectivity of Ion-Exchange Membranes

The application of pulsed electric field (PEF) in electrodialysis has been proven to be efficient for a number of effects: increasing mass transfer rate, mitigation of scaling and fouling, reducing water splitting. Recently, the improvement of the membrane permselectivity for specific counterions was discovered experimentally by the group of Laurent Bazinet (N. Lemay et al. J. Memb. Sci. 604, 117878 (2020)). To better understanding the effect of PEF in electrodialysis, simulations were performed using a non-stationary mathematical model based on the Nernst–Planck and Poisson equations. For the first time, it was not only the condition used when the current density is specified but also the condition when the voltage is set. A membrane and two adjacent diffusion layers are considered. It is shown that when applying the regime used by Lemay et al. (the same current density in conventional continuous current (CC) mode and during the pulses in PEF mode), there is a significant gain in specific permselectivity. It is explained by a reduction in the membrane concentration polarization in PEF mode. In the CC mode of electrodialysis, increasing current density leads to a loss in specific permselectivity: concentration profiles in the diffusion layers and membrane are formed in such a way that ion diffusion reduces the migration flux of the preferentially transferred ion and increases that of the poorly transferred ion. In PEF mode, the concentration profiles are partially restored during the pauses when the current is zero. However, if a different condition is used than the condition applied by Lemay et al., that is, when the same average current density is applied in both the PEF and CC modes, there is no gain in specific permeability. It is shown that within the framework of the applied mathematical model, the specific selectivity depends only on the average current density and does not depend on the mode of its application (CC or PEF mode).

A novel cell permeability assay for macromolecules

Background Many cell permeabilisation methods to mediate internalisation of various molecules to mammalian or bacterial cells have been developed. However, no size-specific permeability assay suitable for both cell types exists. Results We report the use of intrinsically biotinylated cell components as the target for reporter molecules for assessing permeabilisation. Due to its well-described biotin binding activity, we developed an assay using Streptavidin (SAv) as a molecular weight marker for assessing eukaryotic and prokaryotic cell internalisation, using flow cytometry as a readout. This concept was tested here as part of the development of host DNA depletion strategies for microbiome analysis of formalin-fixed (FF) samples. Host depletion (HD) strategies require differential cell permeabilisation, where mammalian cells but not bacterial cells are permeabilised, and are subsequently treated with a nuclease. Here, the internalisation of a SAv-conjugate was used as a reference for nucleases of similar dimensions. With this assay, it was possible to demonstrate that formalin fixation does not generate pores which allow the introduction of 60 KDa molecules in mammalian or bacterial membranes/envelopes. Among surfactants tested, Saponin derived from Quillaja bark showed the best selectivity for mammalian cell permeabilisation, which, when coupled with Benzonase nuclease, provided the best results for host DNA depletion, representing a new HD strategy for formalin fixed samples. Conclusion The assay presented provides researchers with a sensitive and accessible tool for discerning membrane/cell envelop permeability for different size macromolecules.

A novel cell permeability assay for macromolecules

Background: Many cell permeabilisation methods to mediate internalisation of various molecules to mammalian or bacterial cells have been developed. However, no size-specific permeability assay suitable for both cell types exists. Results: We report the use of intrinsically biotinylated cell components as the target for reporter molecules for assessing permeabilisation. Due to its well-described biotin binding activity, we developed an assay using Streptavidin (SAv) as a molecular weight marker for assessing eukaryotic and prokaryotic cell internalisation, using flow cytometry as a readout. This concept was tested here as part of the development of host DNA depletion strategies for microbiome analysis of formalin-fixed (FF) samples. Host depletion (HD) strategies require differential cell permeabilisation, where mammalian cells but not bacterial cells are permeabilised, and are subsequently treated with a nuclease. Here, the internalisation of a SAv-conjugate was used as a reference for nucleases of similar dimensions. With this assay, it was possible to demonstrate that formalin fixation does not generate pores which allow the introduction of 60 KDa molecules in mammalian or bacterial membranes/envelopes. Among surfactants tested, Saponin derived from Quillaja bark showed the best selectivity for mammalian cell permeabilisation, which, when coupled with Benzonase nuclease, provided the best results for host DNA depletion, representing a new HD strategy for formalin fixed samples.Conclusion: The assay presented provides researchers with a sensitive and accessible tool for discerning membrane/cell envelop permeability for different size macromolecules.

A novel cell permeability assay for macromolecules

Background Many cell permeabilisation methods to mediate internalisation of various molecules to mammalian or bacterial cells have been developed. However, no size-specific permeability assay suitable for all cells exists. Results We report for the first time, use of intrinsically biotinylated cell components as the target for reporter molecules for assessing permeabilisation. Due to its well-described biotin binding activity, we developed an assay using Streptavidin (SAv) as a molecular weight marker for assessing eukaryotic and prokaryotic cell internalisation, using flow cytometry as a readout. This concept was tested here in the development of host DNA depletion strategies for microbiome analysis of formalin-fixed samples. This strategy requires differential cell permeabilisation, where mammalian cells but not bacterial cells are permeabilised, and are subsequently treated with a nuclease. Here, the internalisation of a SAv-conjugate was used as a reference for nucleases of similar dimensions. With this assay, it was possible to demonstrate that FF does not generate pores which allow the introduction of 60 KDa molecules in both mammalian and bacterial membranes/envelopes. Among surfactants tested, Saponin showed the best selectivity for mammalian cell permeabilisation, which, when coupled with Benzonase nuclease, provided the best results for host DNA depletion, representing a new host depletion strategy for formalin fixed samples. Conclusion The assay presented provides researchers with a sensitive and accessible tool for discerning membrane/cell envelop permeability for different size macromolecules.

Experimental Study on Seepage Characteristics of Jurassic Weakly Cemented Sandstone under Water-Rock Interaction

As unconventional water-bearing rocks, the hydraulic conductivity of weakly cemented sandstones can increase by several orders of magnitude during drainages or pumping tests, posing great challenges to water prevention and control of coal mines in northwestern China. In this study, seepage experiments on Jurassic weakly cemented sandstones were performed and the hydraulic conductivity during the seepage process was analyzed. Combined with laboratory test and theoretical analysis methods, the relation between the permeability and micropore structures was studied, and the permeability evolution mechanism of the weakly cemented sandstones was eventually clarified. According to the experimental results, the seepage process can be divided into the saturated seepage stage, the stable seepage stage, and the seepage mutation stage. The hydraulic conductivity increases as the porosity and the mercury extrusion rate increase, but there is no obvious correlation between them that can be identified. In contrast, there is a linear positive correlation between the hydraulic conductivity and the average pore-throat radius. The variation trend of the pore-throat ratio can be used as the main reference indicator for judging whether the seepage mutation occurs in weakly cemented sandstone. Based on the correlation analysis of micropore structures and the hydraulic conductivity, a seepage model of straight capillary was constructed and the theoretical permeability equations of stable seepage stage and seepage mutation stage were proposed. It is concluded that the specific permeability of weakly cemented sandstones is directly proportional to porosity and the square of the average pore-throat radius. A theoretical equation to calculate the specific permeability during the latter two stages was also presented in this paper. Theoretical calculation results are roughly consistent with actual values obtained in the experiments.

Technology of Magnetron Deposition of Nanosized High-Barrier Relative Oxygen Aluminium Oxide Coating on Roll Pet Film

The paper introduces the development of the technology of reactive magnetron deposition of a nanosized aluminum oxide layer on a roll PET film, which provides a low specific permeability relative to oxygen. We describe a large-size magnetron deposition facility and its optimal operating modes, as well as the sequence of operations performed. Within the research, we found that various parameters of magnetron deposition of aluminum oxide affect the characteristics of the nanosized layer. The thicknesses of oxide layers were measured in the range of 20--80 nm, the roughness of their surfaces and their structures and reliefs were estimated. Furthermore, we established the experimental dependence of the specific permeability relative to the oxygen of the oxide layer on the PET substrate on its thickness in the range of 20--80 nm. The study gives its qualitative explanation and describes the specific energy consumption

Establishment of an in vitro placental barrier model cultured under physiologically relevant oxygen levels

The human placental barrier facilitates many key functions during pregnancy, most notably the exchange of all substances between the mother and fetus. However, preclinical models of the placental barrier often lacked the multiple cell layers, syncytialization of the trophoblast cells and the low oxygen levels that are present within the body. Therefore, we aimed to design and develop an in vitro model of the placental barrier that would reinstate these factors and enable improved investigations of barrier function. BeWo placental trophoblastic cells and human umbilical vein endothelial cells were co-cultured on contralateral sides of an extracellular matrix-coated transwell insert to establish a multilayered barrier. Epidermal growth factor and forskolin led to significantly increased multi-nucleation of the BeWo cell layer and increased biochemical markers of syncytial fusion, for example syncytin-1 and hCGβ. Our in vitro placental barrier possessed size-specific permeability, with 4000-Da molecules experiencing greater transport and a lower apparent permeability coefficient than 70 000-Da molecules. We further demonstrated that the BeWo layer had greater resistance to smaller molecules compared to the endothelial layer. Chronic, physiologically low oxygen exposure (3–8%) increased the expression of hypoxia-inducible factor 1α and syncytin-1, further increased multi-nucleation of the BeWo cell layer and decreased barrier permeability only against smaller molecules (457 Da/4000 Da). In conclusion, we built a novel in vitro co-culture model of the placental barrier that possessed size-specific permeability and could function under physiologically low oxygen levels. Importantly, this will enable future researchers to better study the maternal–fetal transport of nutrients and drugs during pregnancy.

Albumin-Enriched Fibrin Hydrogel Embedded in Active Ferromagnetic Networks Improves Osteoblast Differentiation and Vascular Self-Organisation

Porous coatings on prosthetic implants encourage implant fixation. Enhanced fixation may be achieved using a magneto-active porous coating that can deform elastically in vivo on the application of an external magnetic field, straining in-growing bone. Such a coating, made of 444 ferritic stainless steel fibres, was previously characterised in terms of its mechanical and cellular responses. In this work, co-cultures of human osteoblasts and endothelial cells were seeded into a novel fibrin-based hydrogel embedded in a 444 ferritic stainless steel fibre network. Albumin was successfully incorporated into fibrin hydrogels improving the specific permeability and the diffusion of fluorescently tagged dextrans without affecting their Young’s modulus. The beneficial effect of albumin was demonstrated by the upregulation of osteogenic and angiogenic gene expression. Furthermore, mineralisation, extracellular matrix production, and formation of vessel-like structures were enhanced in albumin-enriched fibrin hydrogels compared to fibrin hydrogels. Collectively, the results indicate that the albumin-enriched fibrin hydrogel is a promising bio-matrix for bone tissue engineering and orthopaedic applications.

Albumin-Enriched Fibrin Hydrogel Embedded in Active Ferromagnetic Networks Improves Osteoblast Differentiation and Vascular Self-Organisation

Porous coatings on prosthetic implants encourage implant fixation. Enhanced fixation may be achieved using a magneto-active porous coating that can deform elastically in vivo on application of an external magnetic field, straining in-growing bone. Such coating, made of 444 ferritic stainless steel fibres, was previously characterised in terms of its mechanical and cellular responses. In this work, co-cultures of human osteoblasts and endothelial cells were seeded into a novel fibrin-based hydrogel embedded in a 444 ferritic stainless steel fibre network. Albumin was successfully incorporated into fibrin hydrogels improving the specific permeability and the diffusion of fluorescently-tagged dextrans without affecting their Young’s modulus. The beneficial effect of albumin was demonstrated by upregulation of osteogenic and angiogenic gene expression. Furthermore, mineralisation, extracellular matrix production and formation of vessel-like structures were enhanced in albumin-enriched fibrin hydrogels compared to fibrin hydrogels. Collectively, the results indicate that the albumin-enriched fibrin hydrogel is a promising bio-matrix for bone tissue engineering and orthopaedic applications.