Increasing population growth, improve living standards and industrial development are factors which increase water consumption and waste production. Therefore, the use of treated wastewater in agriculture to overcome the problems of water scarcity, particularly in areas with water shortages, is important. In this study in addition to assessing the performance analysis of Genaveh hospital wastewater treatment plant, The quality of the output of effluent of wastewater treatment plant for use in agriculture based on Ayers water quality guidelines were assessed and its potential adverse effects on soil and plants were evaluated. In this study within six months physical and chemical parameters such as pH, EC, calcium, magnesium, sodium, chloride, nitrate, bicarbonate, TDS and heavy metals (iron, manganese, nickel, cadmium, cobalt, lead and zinc) in the output effluent were determined according to standard methods. According to the obtained results, the use of hospital effluent Genaveh in agriculture in term of concentration of sodium in terms of SAR, bicarbonate, chloride, Adsorbed sodium absorption ratio encountered with low to moderate limitation of electrical conductivity. Severe limitations in terms of nitrate nitrogen and heavy metals concentrations in the range are determined. In general results of this study indicate that to reuse the Genaveh hospital effluent in agriculture continuous monitoring of the output of effluent of wastewater treatment plant to meet environmental standards is essential.