scholarly journals Measurement of intracellular sodium concentration and sodium transport in Escherichia coli by 23Na nuclear magnetic resonance.

1986 ◽  
Vol 261 (7) ◽  
pp. 3288-3294 ◽  
Author(s):  
A M Castle ◽  
R M Macnab ◽  
R G Shulman
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Sodium Transport ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Intracellular Sodium Concentration ◽  
Nuclear Magnetic

Nuclear magnetic resonance studies of intracellular ions in perfused frog heart

1987 ◽  
Vol 252 (6) ◽  
pp. H1138-H1146
Author(s):  
D. Burstein ◽  
E. T. Fossel
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Sodium Concentration ◽  
Shift Reagent ◽  
Intracellular Sodium ◽  
Frog Heart ◽  
Intracellular Potassium ◽  
Calcium Buffer ◽  
Nmr Methods ◽  
Nuclear Magnetic

Intracellular sodium, potassium, and lithium were observed in a perfused frog heart by nuclear magnetic resonance (NMR) spectroscopy. A perfusate buffer containing the shift reagent, dysprosium tripolyphosphate, was used in combination with mathematical filtering or presaturation of the extracellular resonance to separate the intra- and extracellular sodium NMR signals. Addition of 10 microM ouabain to the perfusate, perfusion with a zero potassium, low-calcium buffer, and replacement of 66% of the perfusate sodium with lithium resulted in the following percent changes in the intracellular sodium levels (mean +/- SD): ouabain + 460 +/- 60% (n = 6), zero potassium + 300 +/- 30% (n = 3), and lithium - 51 +/- 6% (n = 3). An increase of 45% in the intracellular sodium was observed when changing the pacing rate from 0 to 60 beats/min (with proportional changes for intermediate pacing rates). The ratio of intracellular potassium to sodium concentration was determined to be 2.3 by NMR, indicating that a substantial amount of the intracellular potassium is undetectable with these NMR methods. In addition, intracellular lithium was observed during perfusion with a lithium-containing perfusate.

Proton correlation nuclear magnetic resonance study of anaerobic metabolism of Escherichia coli

Biochemistry ◽  
1978 ◽  
Vol 17 (22) ◽  
pp. 4742-4745 ◽  
Author(s):  
T. Ogino ◽  
Y. Arata ◽  
S. Fujiwara ◽  
H. Shoun ◽  
T. Beppu
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Anaerobic Metabolism ◽  
Nuclear Magnetic Resonance Study ◽  
Magnetic Resonance Study ◽  
Nuclear Magnetic

Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia Coli

2011 ◽  
Vol 39 (8) ◽  
pp. 1186-1194 ◽  
Author(s):  
Yang-Fang YE ◽  
Li-Min ZHANG ◽  
Yan-Peng AN ◽  
Fu-Hua HAO ◽  
Hui-Ru TANG
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nuclear Magnetic
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