Restriction endonuclease accessibility of the developmentally regulated goat gamma-, beta C-, and beta A-globin genes in chromatin. Differences in 5' regions which show unusually high sequence homology.

Traumatic injury, including bone fracture, is, to date, one of the leading causes of koala mortality in the South East Queensland region of Australia. Further, the specialist diet of koalas, which is restricted to certain Eucalyptus spp., may impact their normal bone physiology. Considering the dramatic koala population decline and high incidence of trauma, a greater understanding of koala bone physiology may support conservation. We retrieved from GenBank the protein sequences of parathyroid hormone (PTH), osteocalcin (OCN), and tissue-nonspecific alkaline phosphatase (TNALP) in human, dog, cattle, horse, koala, and gray short-tailed opossum. After homology was determined, plasma samples from 13 koalas were analyzed with human PTH, OCN, and bone-specific ALP (BALP) assay kits. Although koala PTH exhibited relatively low sequence homology with placental mammals, high sequence homology between humans and koalas was observed for both OCN and TNALP, and successful cross-reactivity was achieved using human enzyme immunoassay kits for detection of OCN and BALP biomarkers in koala plasma. However, we identified no correlation between OCN and BALP concentrations of healthy and trauma-affected koalas ( p = 0.66 and p = 0.79, respectively). Further analysis of OCN and BALP in healthy and diseased koalas will allow a better understanding of bone physiology in this unique marsupial.

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Biological Activities and Structural Properties of the Atypical Bacteriocins Mesenterocin 52B and Leucocin B-TA33a

Applied and Environmental Microbiology ◽

10.1128/aem.67.4.1418-1422.2001 ◽

2001 ◽

Vol 67 (4) ◽

pp. 1418-1422 ◽

Cited By ~ 13

Author(s):

C. Corbier ◽

F. Krier ◽

G. Mulliert ◽

B. Vitoux ◽

A.-M. Revol-Junelles

Keyword(s):

Circular Dichroism ◽

Structural Properties ◽

Sequence Homology ◽

Synthetic Peptides ◽

Biological Activities ◽

Modes Of Action ◽

Amphiphilic Helix ◽

High Sequence Homology ◽

Circular Dichroïsm

ABSTRACT The antibacterial spectra and modes of action of synthetic peptides corresponding to mesenterocin 52B and leucocin B-TA33a greatly differ despite their high sequence homology. Circular dichroism experiments establish the capacity of each of these two peptides to partly fold into an amphiphilic helix that might be crucial for their adsorption at lipophilic-hydrophilic interfaces.

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High sequence homology of the PrP gene in mule deer and Rocky Mountain elk

The Lancet ◽

10.1016/s0140-6736(05)62387-2 ◽

1997 ◽

Vol 350 (9072) ◽

pp. 219-220 ◽

Cited By ~ 12

Author(s):

Larisa Cervenáková ◽

Robert Rohwer ◽

Elizabeth S Williams ◽

Paul Brown ◽

D Carleton Gajdusek

Keyword(s):

Sequence Homology ◽

Mule Deer ◽

Rocky Mountain ◽

High Sequence Homology ◽

Rocky Mountain Elk ◽

Prp Gene

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The rare alpha-thalassemia-1 of blacks is a zeta alpha-thalassemia-1 associated with deletion of all alpha- and zeta-globin genes

Blood ◽

10.1182/blood.v63.5.1253.bloodjournal6351253 ◽

1984 ◽

Vol 63 (5) ◽

pp. 1253-1257

Author(s):

AE Felice ◽

MP Cleek ◽

K McKie ◽

V McKie ◽

TH Huisman

Keyword(s):

Restriction Endonuclease ◽

Globin Gene ◽

Hydrops Fetalis ◽

Biochemical Data ◽

Globin Genes ◽

Gene Complex ◽

Alpha Thalassemia ◽

Zeta Globin Gene ◽

Endonuclease Mapping ◽

Hbh Disease

Restriction endonuclease mapping with alpha and zeta-globin gene probes showed differences between the alpha-thalassemia-1 (alpha-thal-1) condition in two patients with HbH disease. One patient had the rare black type of alpha-thal-1 together with alpha-thal-2 and HbS heterozygosities. The second patient was a Laotian child with HbE, Hb Constant Spring (alpha-thal-2), and alpha-thal-1 heterozygosities. The diagnoses were based on clinical, hematologic, and biochemical data. Whereas DNA fragments hybridizing to a zeta-probe were obtained from the Laotian type of alpha-thal-1, neither alpha nor zeta-gene fragments could be identified deriving from the black type of alpha-thal-1. Therefore, the black type of alpha-thal-1 is associated with a deletion of the entire zeta 2-psi zeta-psi alpha-alpha 2-alpha 1 gene complex and can be considered a zeta alpha-thal-1. It is likely that homozygosity for such a condition will lead to embryonic wastage, explaining the absence of hydrops fetalis in blacks.

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